Generation of an alpaca‐derived nanobody recognizing γ‐H2AX
Post‐translational modifications are difficult to visualize in living cells and are conveniently analyzed using antibodies. Single‐chain antibody fragments derived from alpacas and called nanobodies can be expressed and bind to the target antigenic sites in living cells. As a proof of concept, we ge...
Main Authors: | Malini Rajan, Oliver Mortusewicz, Ulrich Rothbauer, Florian D. Hastert, Katrin Schmidthals, Alexander Rapp, Heinrich Leonhardt, M. Cristina Cardoso |
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Format: | Article |
Language: | English |
Published: |
Wiley
2015-01-01
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Series: | FEBS Open Bio |
Subjects: | |
Online Access: | https://doi.org/10.1016/j.fob.2015.09.005 |
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