Synthesis and Characterization of Diosgenin Encapsulated Poly-ε-Caprolactone-Pluronic Nanoparticles and Its Effect on Brain Cancer Cells
Diosgenin encapsulated PCL-Pluronic nanoparticles (PCL-F68-D-NPs) were developed using the nanoprecipitation method to improve performance in brain cancer (glioblastoma) therapy. The nanoparticles were characterized by dynamic light scattering (DLS)/Zeta potential, Fourier-transform infrared (FTIR)...
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MDPI AG
2021-04-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/13/8/1322 |
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| author | Bijuli Rabha Kaushik Kumar Bharadwaj Debabrat Baishya Tanmay Sarkar Hisham Atan Edinur Siddhartha Pati |
| author_facet | Bijuli Rabha Kaushik Kumar Bharadwaj Debabrat Baishya Tanmay Sarkar Hisham Atan Edinur Siddhartha Pati |
| author_sort | Bijuli Rabha |
| collection | DOAJ |
| description | Diosgenin encapsulated PCL-Pluronic nanoparticles (PCL-F68-D-NPs) were developed using the nanoprecipitation method to improve performance in brain cancer (glioblastoma) therapy. The nanoparticles were characterized by dynamic light scattering (DLS)/Zeta potential, Fourier-transform infrared (FTIR) spectra, X-ray diffraction (XRD), Field Emission Scanning Electron Microscopy (FESEM), and Transmission electron microscopy (TEM). The encapsulation efficiency, loading efficiency, and yield were calculated. The in vitro release rate was determined, and the kinetic model of diosgenin release was plotted and ascertained. The cytotoxicity was checked by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide)assay against U87-MG cells (glioblastoma cell lines). The obtained nanoparticles demonstrated good size distribution, stability, morphology, chemical, and mechanical properties. The nanoparticles also possessed high encapsulation efficiency, loading efficiency, and yield. The release rate of Diosgenin was shown in a sustained manner. The in vitro cytotoxicity of PCL-F68-D-NPs showed higher toxicity against U87-MG cells than free Diosgenin. |
| first_indexed | 2024-03-10T12:13:45Z |
| format | Article |
| id | doaj.art-ba2256d0ae7f40d4bf80b19f1ba8d2bc |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-10T12:13:45Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-ba2256d0ae7f40d4bf80b19f1ba8d2bc2023-11-21T16:03:21ZengMDPI AGPolymers2073-43602021-04-01138132210.3390/polym13081322Synthesis and Characterization of Diosgenin Encapsulated Poly-ε-Caprolactone-Pluronic Nanoparticles and Its Effect on Brain Cancer CellsBijuli Rabha0Kaushik Kumar Bharadwaj1Debabrat Baishya2Tanmay Sarkar3Hisham Atan Edinur4Siddhartha Pati5Department of Bioengineering and Technology, Gauhati University, Guwahati, Assam 781014, IndiaDepartment of Bioengineering and Technology, Gauhati University, Guwahati, Assam 781014, IndiaDepartment of Bioengineering and Technology, Gauhati University, Guwahati, Assam 781014, IndiaMalda Polytechnic, West Bengal State Council of Technical Education, Govt. of West Bengal, Malda, West Bengal 732102, IndiaHealth Campus, School of Health Sciences, Universiti Sains Malaysia, Kubang Kerian, Kelantan 16150, MalaysiaCentre of Excellence, Khallikote University, Berhampur, Ganjam, Odisha 761008, IndiaDiosgenin encapsulated PCL-Pluronic nanoparticles (PCL-F68-D-NPs) were developed using the nanoprecipitation method to improve performance in brain cancer (glioblastoma) therapy. The nanoparticles were characterized by dynamic light scattering (DLS)/Zeta potential, Fourier-transform infrared (FTIR) spectra, X-ray diffraction (XRD), Field Emission Scanning Electron Microscopy (FESEM), and Transmission electron microscopy (TEM). The encapsulation efficiency, loading efficiency, and yield were calculated. The in vitro release rate was determined, and the kinetic model of diosgenin release was plotted and ascertained. The cytotoxicity was checked by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide)assay against U87-MG cells (glioblastoma cell lines). The obtained nanoparticles demonstrated good size distribution, stability, morphology, chemical, and mechanical properties. The nanoparticles also possessed high encapsulation efficiency, loading efficiency, and yield. The release rate of Diosgenin was shown in a sustained manner. The in vitro cytotoxicity of PCL-F68-D-NPs showed higher toxicity against U87-MG cells than free Diosgenin.https://www.mdpi.com/2073-4360/13/8/1322Diosgeninnanoparticlesglioblastoma cell linesencapsulation |
| spellingShingle | Bijuli Rabha Kaushik Kumar Bharadwaj Debabrat Baishya Tanmay Sarkar Hisham Atan Edinur Siddhartha Pati Synthesis and Characterization of Diosgenin Encapsulated Poly-ε-Caprolactone-Pluronic Nanoparticles and Its Effect on Brain Cancer Cells Polymers Diosgenin nanoparticles glioblastoma cell lines encapsulation |
| title | Synthesis and Characterization of Diosgenin Encapsulated Poly-ε-Caprolactone-Pluronic Nanoparticles and Its Effect on Brain Cancer Cells |
| title_full | Synthesis and Characterization of Diosgenin Encapsulated Poly-ε-Caprolactone-Pluronic Nanoparticles and Its Effect on Brain Cancer Cells |
| title_fullStr | Synthesis and Characterization of Diosgenin Encapsulated Poly-ε-Caprolactone-Pluronic Nanoparticles and Its Effect on Brain Cancer Cells |
| title_full_unstemmed | Synthesis and Characterization of Diosgenin Encapsulated Poly-ε-Caprolactone-Pluronic Nanoparticles and Its Effect on Brain Cancer Cells |
| title_short | Synthesis and Characterization of Diosgenin Encapsulated Poly-ε-Caprolactone-Pluronic Nanoparticles and Its Effect on Brain Cancer Cells |
| title_sort | synthesis and characterization of diosgenin encapsulated poly ε caprolactone pluronic nanoparticles and its effect on brain cancer cells |
| topic | Diosgenin nanoparticles glioblastoma cell lines encapsulation |
| url | https://www.mdpi.com/2073-4360/13/8/1322 |
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