Reliable multiplex sequencing with rare index mis-assignment on DNB-based NGS platform

Reliable multiplex sequencing with rare index mis-assignment on DNB-based NGS platform

Abstract Background Massively-parallel-sequencing, coupled with sample multiplexing, has made genetic tests broadly affordable. However, intractable index mis-assignments (commonly exceeds 1%) were repeatedly reported on some widely used sequencing platforms. Results Here, we investigated this quali...

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Bibliographic Details
Main Authors: Qiaoling Li, Xia Zhao, Wenwei Zhang, Lin Wang, Jingjing Wang, Dongyang Xu, Zhiying Mei, Qiang Liu, Shiyi Du, Zhanqing Li, Xinming Liang, Xiaman Wang, Hanmin Wei, Pengjuan Liu, Jing Zou, Hanjie Shen, Ao Chen, Snezana Drmanac, Jia Sophie Liu, Li Li, Hui Jiang, Yongwei Zhang, Jian Wang, Huanming Yang, Xun Xu, Radoje Drmanac, Yuan Jiang
Format: Article
Language:English
Published: BMC 2019-03-01
Series:BMC Genomics
Subjects:
NGS
Multiplex sequencing
Rare index mis-assignment
DNA nanoball technology
Online Access:http://link.springer.com/article/10.1186/s12864-019-5569-5

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