Effect of lipocalin-2 on angiogenesis of murine retinal vascular endothelial cells
AIM: To explore the role of lipocalin-2(LCN-2)in retinal angiogenesis <i>in vitro</i> by observing its effects on proliferation, migration and capillary-like tube formation of murine retinal vascular endothelial cells(RVECs). <p>METHODS: Well cultured RVECs were divided to differen...
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| Format: | Article |
| Language: | English |
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Press of International Journal of Ophthalmology (IJO PRESS)
2018-09-01
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| Series: | Guoji Yanke Zazhi |
| Subjects: | |
| Online Access: | http://ies.ijo.cn/cn_publish/2018/9/201809005.pdf |
| _version_ | 1818846249620602880 |
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| author | Guo-Min Yao Rong Li Jin Tian |
| author_facet | Guo-Min Yao Rong Li Jin Tian |
| author_sort | Guo-Min Yao |
| collection | DOAJ |
| description | AIM: To explore the role of lipocalin-2(LCN-2)in retinal angiogenesis <i>in vitro</i> by observing its effects on proliferation, migration and capillary-like tube formation of murine retinal vascular endothelial cells(RVECs). <p>METHODS: Well cultured RVECs were divided to different groups which were treated with 0, 5, 10 μmol/L LCN-2 for 48h, respectively. Cell proliferation, migration and tube formation were detected by using the EDU assay, transwell assay and matrigel assay, respectively. <p>RESULTS: The cell proliferation rate was promoted in both low and high dose of LCN-2 groups compared to the control cells(<i>P</i><0.05). The number of migrated cells in both LCN-2 groups was significantly larger than that of the control group(<i>P</i><0.05). The number of capillary-like tube structures of both LCN-2 groups was significantly larger than that of the control cells(<i>P</i><0.05). In addition, cell proliferation, migration and tube formation were all increased with the increase of LCN-2 concentration. <p>CONCLUSION: LCN-2 could obviously promote the angiogenesis capacity of RVECs, suggesting that LCN-2 is an important pro-angiogenic factor in retinal angiogenesis. |
| first_indexed | 2024-12-19T05:42:33Z |
| format | Article |
| id | doaj.art-ba259ccca5774995bcc8e9216517aaa9 |
| institution | Directory Open Access Journal |
| issn | 1672-5123 1672-5123 |
| language | English |
| last_indexed | 2024-12-19T05:42:33Z |
| publishDate | 2018-09-01 |
| publisher | Press of International Journal of Ophthalmology (IJO PRESS) |
| record_format | Article |
| series | Guoji Yanke Zazhi |
| spelling | doaj.art-ba259ccca5774995bcc8e9216517aaa92022-12-21T20:33:58ZengPress of International Journal of Ophthalmology (IJO PRESS)Guoji Yanke Zazhi1672-51231672-51232018-09-011891578158210.3980/j.issn.1672-5123.2018.9.05Effect of lipocalin-2 on angiogenesis of murine retinal vascular endothelial cellsGuo-Min Yao0Rong Li1Jin Tian2Department of Ophthalmology, The First Affiliated Hospital of Xi'an Medical University, Xi'an 710077, Shaanxi Province, ChinaDepartment of Ophthalmology, The First Affiliated Hospital of Xi'an Medical University, Xi'an 710077, Shaanxi Province, ChinaDepartment of Ophthalmology, Weinan Central Hospital, Weinan 714000, Shaanxi Province, ChinaAIM: To explore the role of lipocalin-2(LCN-2)in retinal angiogenesis <i>in vitro</i> by observing its effects on proliferation, migration and capillary-like tube formation of murine retinal vascular endothelial cells(RVECs). <p>METHODS: Well cultured RVECs were divided to different groups which were treated with 0, 5, 10 μmol/L LCN-2 for 48h, respectively. Cell proliferation, migration and tube formation were detected by using the EDU assay, transwell assay and matrigel assay, respectively. <p>RESULTS: The cell proliferation rate was promoted in both low and high dose of LCN-2 groups compared to the control cells(<i>P</i><0.05). The number of migrated cells in both LCN-2 groups was significantly larger than that of the control group(<i>P</i><0.05). The number of capillary-like tube structures of both LCN-2 groups was significantly larger than that of the control cells(<i>P</i><0.05). In addition, cell proliferation, migration and tube formation were all increased with the increase of LCN-2 concentration. <p>CONCLUSION: LCN-2 could obviously promote the angiogenesis capacity of RVECs, suggesting that LCN-2 is an important pro-angiogenic factor in retinal angiogenesis.http://ies.ijo.cn/cn_publish/2018/9/201809005.pdflipocalin-2angiogenesisretinal endothelial cellretinal endothelial cell |
| spellingShingle | Guo-Min Yao Rong Li Jin Tian Effect of lipocalin-2 on angiogenesis of murine retinal vascular endothelial cells Guoji Yanke Zazhi lipocalin-2 angiogenesis retinal endothelial cell retinal endothelial cell |
| title | Effect of lipocalin-2 on angiogenesis of murine retinal vascular endothelial cells |
| title_full | Effect of lipocalin-2 on angiogenesis of murine retinal vascular endothelial cells |
| title_fullStr | Effect of lipocalin-2 on angiogenesis of murine retinal vascular endothelial cells |
| title_full_unstemmed | Effect of lipocalin-2 on angiogenesis of murine retinal vascular endothelial cells |
| title_short | Effect of lipocalin-2 on angiogenesis of murine retinal vascular endothelial cells |
| title_sort | effect of lipocalin 2 on angiogenesis of murine retinal vascular endothelial cells |
| topic | lipocalin-2 angiogenesis retinal endothelial cell retinal endothelial cell |
| url | http://ies.ijo.cn/cn_publish/2018/9/201809005.pdf |
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