Advanced Biomimetic Nanostructured Microelectrode Arrays for Enhanced Extracellular Recordings of Enteric Neurons
Abstract Microelectrode surfaces covered with nanostructures derived from components of extracellular matrix, such as collagen fibers, have shown immense beneficial effects in promoting neuronal growth and cellular signaling. Synthetic nanostructures mimicking the features of biological nanostructur...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley-VCH
2023-06-01
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| Series: | Advanced Materials Interfaces |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/admi.202300023 |
| _version_ | 1797736708801822720 |
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| author | Bharat Nowduri Steven Schulte Negin Adavoudi Jolfaei Dominique Decker Holger Rabe Karl‐Herbert Schäfer Monika Saumer |
| author_facet | Bharat Nowduri Steven Schulte Negin Adavoudi Jolfaei Dominique Decker Holger Rabe Karl‐Herbert Schäfer Monika Saumer |
| author_sort | Bharat Nowduri |
| collection | DOAJ |
| description | Abstract Microelectrode surfaces covered with nanostructures derived from components of extracellular matrix, such as collagen fibers, have shown immense beneficial effects in promoting neuronal growth and cellular signaling. Synthetic nanostructures mimicking the features of biological nanostructures with durable conductive materials could promote the cell adhesion on microelectrode surfaces by providing topographical cues and simultaneously improve the charge transfer properties by reducing its global impedance. Therefore, an advanced nanostructuring method mimicking the structural and organizational features of natural collagen fibers onto metallic microelectrode surfaces has been presented here, which is adapted from previous technological achievements of the group and is based on nanoimprint lithography and gold electroplating. Surface characterization methods reveal an increase in surface area between 20% and 68% on the microelectrodes fabricated with two different nanostructure heights. Impedance spectroscopy measurements reveal reduction in impedance magnitude (at 1 kHz) between 22% and 41%, depending upon the nanostructure height and density on the microelectrode, which should subsequently modulate its charge transfer properties for biosensing application. Cell adhesion analysis performed with seal impedance measurements reveals a tighter coupling of enteric neurons on the nanostructured microelectrodes. Finally, extracellular recordings from enteric neurons exhibit a significant improvement in spike detection properties of the nanostructured microelectrodes. |
| first_indexed | 2024-03-12T13:17:48Z |
| format | Article |
| id | doaj.art-ba26519bbf904ffea336e5b61809aca2 |
| institution | Directory Open Access Journal |
| issn | 2196-7350 |
| language | English |
| last_indexed | 2024-03-12T13:17:48Z |
| publishDate | 2023-06-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | Advanced Materials Interfaces |
| spelling | doaj.art-ba26519bbf904ffea336e5b61809aca22023-08-26T12:45:57ZengWiley-VCHAdvanced Materials Interfaces2196-73502023-06-011016n/an/a10.1002/admi.202300023Advanced Biomimetic Nanostructured Microelectrode Arrays for Enhanced Extracellular Recordings of Enteric NeuronsBharat Nowduri0Steven Schulte1Negin Adavoudi Jolfaei2Dominique Decker3Holger Rabe4Karl‐Herbert Schäfer5Monika Saumer6Department of Microsystems Technology University of Applied Sciences Kaiserslautern Amerikastraße 1 66482 Zweibrücken GermanyDepartment of Microsystems Technology University of Applied Sciences Kaiserslautern Amerikastraße 1 66482 Zweibrücken GermanyDivision of Neurobiology and Zoology Technical University of Kaiserslautern Erwin Schrödinger‐Straße 13 D 67663 Kaiserslautern GermanyDepartment of Microsystems Technology University of Applied Sciences Kaiserslautern Amerikastraße 1 66482 Zweibrücken GermanyDepartment of Microsystems Technology University of Applied Sciences Kaiserslautern Amerikastraße 1 66482 Zweibrücken GermanyDepartment of Microsystems Technology University of Applied Sciences Kaiserslautern Amerikastraße 1 66482 Zweibrücken GermanyDepartment of Microsystems Technology University of Applied Sciences Kaiserslautern Amerikastraße 1 66482 Zweibrücken GermanyAbstract Microelectrode surfaces covered with nanostructures derived from components of extracellular matrix, such as collagen fibers, have shown immense beneficial effects in promoting neuronal growth and cellular signaling. Synthetic nanostructures mimicking the features of biological nanostructures with durable conductive materials could promote the cell adhesion on microelectrode surfaces by providing topographical cues and simultaneously improve the charge transfer properties by reducing its global impedance. Therefore, an advanced nanostructuring method mimicking the structural and organizational features of natural collagen fibers onto metallic microelectrode surfaces has been presented here, which is adapted from previous technological achievements of the group and is based on nanoimprint lithography and gold electroplating. Surface characterization methods reveal an increase in surface area between 20% and 68% on the microelectrodes fabricated with two different nanostructure heights. Impedance spectroscopy measurements reveal reduction in impedance magnitude (at 1 kHz) between 22% and 41%, depending upon the nanostructure height and density on the microelectrode, which should subsequently modulate its charge transfer properties for biosensing application. Cell adhesion analysis performed with seal impedance measurements reveals a tighter coupling of enteric neurons on the nanostructured microelectrodes. Finally, extracellular recordings from enteric neurons exhibit a significant improvement in spike detection properties of the nanostructured microelectrodes.https://doi.org/10.1002/admi.202300023biomimeticscell adhesionextracellular signalingimpedance spectroscopymicroelectrode arrays |
| spellingShingle | Bharat Nowduri Steven Schulte Negin Adavoudi Jolfaei Dominique Decker Holger Rabe Karl‐Herbert Schäfer Monika Saumer Advanced Biomimetic Nanostructured Microelectrode Arrays for Enhanced Extracellular Recordings of Enteric Neurons Advanced Materials Interfaces biomimetics cell adhesion extracellular signaling impedance spectroscopy microelectrode arrays |
| title | Advanced Biomimetic Nanostructured Microelectrode Arrays for Enhanced Extracellular Recordings of Enteric Neurons |
| title_full | Advanced Biomimetic Nanostructured Microelectrode Arrays for Enhanced Extracellular Recordings of Enteric Neurons |
| title_fullStr | Advanced Biomimetic Nanostructured Microelectrode Arrays for Enhanced Extracellular Recordings of Enteric Neurons |
| title_full_unstemmed | Advanced Biomimetic Nanostructured Microelectrode Arrays for Enhanced Extracellular Recordings of Enteric Neurons |
| title_short | Advanced Biomimetic Nanostructured Microelectrode Arrays for Enhanced Extracellular Recordings of Enteric Neurons |
| title_sort | advanced biomimetic nanostructured microelectrode arrays for enhanced extracellular recordings of enteric neurons |
| topic | biomimetics cell adhesion extracellular signaling impedance spectroscopy microelectrode arrays |
| url | https://doi.org/10.1002/admi.202300023 |
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