In Vitro Assessment of Anticoccidials: Methods and Molecules
Avian coccidiosis is a disease causing considerable economic losses in the poultry industry. It is caused by <i>Eimeria</i> spp., protozoan parasites characterized by an exogenous–endogenous lifecycle. In vitro research on these pathogens is very complicated and lacks standardization. Th...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-06-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/11/7/1962 |
| _version_ | 1797528221298720768 |
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| author | Martina Felici Benedetta Tugnoli Andrea Piva Ester Grilli |
| author_facet | Martina Felici Benedetta Tugnoli Andrea Piva Ester Grilli |
| author_sort | Martina Felici |
| collection | DOAJ |
| description | Avian coccidiosis is a disease causing considerable economic losses in the poultry industry. It is caused by <i>Eimeria</i> spp., protozoan parasites characterized by an exogenous–endogenous lifecycle. In vitro research on these pathogens is very complicated and lacks standardization. This review provides a description of the main in vitro protocols so far assessed focusing on the exogenous phase, with oocyst viability and sporulation assays, and on the endogenous phase, with invasion and developmental assays in cell cultures and in ovo. An overview of these in vitro applications to screen both old and new remedies and to understand the relative mode of action is also discussed. |
| first_indexed | 2024-03-10T09:55:06Z |
| format | Article |
| id | doaj.art-ba26df13302e4704a47580c04407eb09 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T09:55:06Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-ba26df13302e4704a47580c04407eb092023-11-22T02:24:55ZengMDPI AGAnimals2076-26152021-06-01117196210.3390/ani11071962In Vitro Assessment of Anticoccidials: Methods and MoleculesMartina Felici0Benedetta Tugnoli1Andrea Piva2Ester Grilli3Dipartimento di Scienze Mediche Veterinarie (DIMEVET), Università di Bologna, Via Tolara di Sopra 50, 40064 Ozzano dell’Emilia, ItalyVetagro S.p.A., Via Porro 2, 42124 Reggio Emilia, ItalyDipartimento di Scienze Mediche Veterinarie (DIMEVET), Università di Bologna, Via Tolara di Sopra 50, 40064 Ozzano dell’Emilia, ItalyDipartimento di Scienze Mediche Veterinarie (DIMEVET), Università di Bologna, Via Tolara di Sopra 50, 40064 Ozzano dell’Emilia, ItalyAvian coccidiosis is a disease causing considerable economic losses in the poultry industry. It is caused by <i>Eimeria</i> spp., protozoan parasites characterized by an exogenous–endogenous lifecycle. In vitro research on these pathogens is very complicated and lacks standardization. This review provides a description of the main in vitro protocols so far assessed focusing on the exogenous phase, with oocyst viability and sporulation assays, and on the endogenous phase, with invasion and developmental assays in cell cultures and in ovo. An overview of these in vitro applications to screen both old and new remedies and to understand the relative mode of action is also discussed.https://www.mdpi.com/2076-2615/11/7/1962<i>Eimeria</i>poultryin vitrocell culturebotanicalsessential oils |
| spellingShingle | Martina Felici Benedetta Tugnoli Andrea Piva Ester Grilli In Vitro Assessment of Anticoccidials: Methods and Molecules Animals <i>Eimeria</i> poultry in vitro cell culture botanicals essential oils |
| title | In Vitro Assessment of Anticoccidials: Methods and Molecules |
| title_full | In Vitro Assessment of Anticoccidials: Methods and Molecules |
| title_fullStr | In Vitro Assessment of Anticoccidials: Methods and Molecules |
| title_full_unstemmed | In Vitro Assessment of Anticoccidials: Methods and Molecules |
| title_short | In Vitro Assessment of Anticoccidials: Methods and Molecules |
| title_sort | in vitro assessment of anticoccidials methods and molecules |
| topic | <i>Eimeria</i> poultry in vitro cell culture botanicals essential oils |
| url | https://www.mdpi.com/2076-2615/11/7/1962 |
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