Photodynamic Properties of CdSe/CdS Quantum Dots in Intracellular Media

CdSe/CdS quantum dots (QDs) were seeded into Jurkat cells using polyethylene glycol (PEG-1500) at different treatment times. Fluorescence microscopy images show that some QDs stick to the surface of the cells, while others appeared to be inside the cells. As it is difficult to ascertain whether the...

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Main Authors: Thanh Binh Nguyen, Thi Bich Vu, Dinh Cong Nguyen, Thi Thao Do, Hong Minh Pham, Marilou Cadatal-Raduban
Format: Article
Language:English
Published: MDPI AG 2020-06-01
Series:Applied Sciences
Subjects:
Online Access:https://www.mdpi.com/2076-3417/10/11/3988
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author Thanh Binh Nguyen
Thi Bich Vu
Dinh Cong Nguyen
Thi Thao Do
Hong Minh Pham
Marilou Cadatal-Raduban
author_facet Thanh Binh Nguyen
Thi Bich Vu
Dinh Cong Nguyen
Thi Thao Do
Hong Minh Pham
Marilou Cadatal-Raduban
author_sort Thanh Binh Nguyen
collection DOAJ
description CdSe/CdS quantum dots (QDs) were seeded into Jurkat cells using polyethylene glycol (PEG-1500) at different treatment times. Fluorescence microscopy images show that some QDs stick to the surface of the cells, while others appeared to be inside the cells. As it is difficult to ascertain whether the QDs are indeed inside the cells or just behind the cells, additional spectroscopic studies were performed. Photoluminescence spectra show that the fluorescence intensities of the CdSe/CdS QDs are different between samples at different treatment times. Interestingly, the fluorescence lifetimes are also different. This confirms the interaction between the CdSe/CdS QDs and the intracellular media and that the QDs were successfully seeded into the cells.
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spelling doaj.art-ba29336729984dc5a35ae94716c80e7f2023-11-20T03:15:14ZengMDPI AGApplied Sciences2076-34172020-06-011011398810.3390/app10113988Photodynamic Properties of CdSe/CdS Quantum Dots in Intracellular MediaThanh Binh Nguyen0Thi Bich Vu1Dinh Cong Nguyen2Thi Thao Do3Hong Minh Pham4Marilou Cadatal-Raduban5Institute of Physics, Vietnam Academy of Science and Technology, Hanoi 100000, VietnamInstitute of Physics, Vietnam Academy of Science and Technology, Hanoi 100000, VietnamInstitute of Physics, Vietnam Academy of Science and Technology, Hanoi 100000, VietnamInstitute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi 100000, VietnamInstitute of Physics, Vietnam Academy of Science and Technology, Hanoi 100000, VietnamSchool of Natural and Computational Sciences (SNCS), Massey University, Albany, Auckland 0632, New ZealandCdSe/CdS quantum dots (QDs) were seeded into Jurkat cells using polyethylene glycol (PEG-1500) at different treatment times. Fluorescence microscopy images show that some QDs stick to the surface of the cells, while others appeared to be inside the cells. As it is difficult to ascertain whether the QDs are indeed inside the cells or just behind the cells, additional spectroscopic studies were performed. Photoluminescence spectra show that the fluorescence intensities of the CdSe/CdS QDs are different between samples at different treatment times. Interestingly, the fluorescence lifetimes are also different. This confirms the interaction between the CdSe/CdS QDs and the intracellular media and that the QDs were successfully seeded into the cells.https://www.mdpi.com/2076-3417/10/11/3988photodynamic propertiestime-resolved fluorescencefluorescence resonance energy transfer
spellingShingle Thanh Binh Nguyen
Thi Bich Vu
Dinh Cong Nguyen
Thi Thao Do
Hong Minh Pham
Marilou Cadatal-Raduban
Photodynamic Properties of CdSe/CdS Quantum Dots in Intracellular Media
Applied Sciences
photodynamic properties
time-resolved fluorescence
fluorescence resonance energy transfer
title Photodynamic Properties of CdSe/CdS Quantum Dots in Intracellular Media
title_full Photodynamic Properties of CdSe/CdS Quantum Dots in Intracellular Media
title_fullStr Photodynamic Properties of CdSe/CdS Quantum Dots in Intracellular Media
title_full_unstemmed Photodynamic Properties of CdSe/CdS Quantum Dots in Intracellular Media
title_short Photodynamic Properties of CdSe/CdS Quantum Dots in Intracellular Media
title_sort photodynamic properties of cdse cds quantum dots in intracellular media
topic photodynamic properties
time-resolved fluorescence
fluorescence resonance energy transfer
url https://www.mdpi.com/2076-3417/10/11/3988
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