Biomimetic hydrogel blanket for conserving and recovering intrinsic cell properties
Abstract Background Cells in the human body experience different growth environments and conditions, such as compressive pressure and oxygen concentrations, depending on the type and location of the tissue. Thus, a culture device that emulates the environment inside the body is required to study cel...
| Main Authors: | , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
American Association for the Advancement of Science (AAAS)
2022-12-01
|
| Series: | Biomaterials Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40824-022-00327-w |
| _version_ | 1797285753672171520 |
|---|---|
| author | Seung-Hoon Um Youngmin Seo Hyunseon Seo Kyungwoo Lee Sun Hwa Park Jung Ho Jeon Jung Yeon Lim Myoung-Ryul Ok Yu-Chan Kim Hyunjung Kim Cheol-Hong Cheon Hyung-Seop Han James R. Edwards Sung Won Kim Hojeong Jeon |
| author_facet | Seung-Hoon Um Youngmin Seo Hyunseon Seo Kyungwoo Lee Sun Hwa Park Jung Ho Jeon Jung Yeon Lim Myoung-Ryul Ok Yu-Chan Kim Hyunjung Kim Cheol-Hong Cheon Hyung-Seop Han James R. Edwards Sung Won Kim Hojeong Jeon |
| author_sort | Seung-Hoon Um |
| collection | DOAJ |
| description | Abstract Background Cells in the human body experience different growth environments and conditions, such as compressive pressure and oxygen concentrations, depending on the type and location of the tissue. Thus, a culture device that emulates the environment inside the body is required to study cells outside the body. Methods A blanket-type cell culture device (Direct Contact Pressing: DCP) was fabricated with an alginate-based hydrogel. Changes in cell morphology due to DCP pressure were observed using a phase contrast microscope. The changes in the oxygen permeability and pressure according to the hydrogel concentration of DCP were analyzed. To compare the effects of DCP with normal or artificial hypoxic cultures, cells were divided based on the culture technique: normal culture, DCP culture device, and artificial hypoxic environment. Changes in phenotype, genes, and glycosaminoglycan amounts according to each environment were evaluated. Based on this, the mechanism of each culture environment on the intrinsic properties of conserving chondrocytes was suggested. Results Chondrocytes live under pressure from the surrounding collagen tissue and experience a hypoxic environment because collagen inhibits oxygen permeability. By culturing the chondrocytes in a DCP environment, the capability of DCP to produce a low-oxygen and physical pressure environment was verified. When human primary chondrocytes, which require pressure and a low-oxygen environment during culture to maintain their innate properties, were cultured using the hydrogel blanket, the original shapes and properties of the chondrocytes were maintained. The intrinsic properties could be recovered even in aged cells that had lost their original cell properties. Conclusions A DCP culture method using a biomimetic hydrogel blanket provides cells with an adjustable physical pressure and a low-oxygen environment. Through this technique, we could maintain the original cellular phenotypes and intrinsic properties of human primary chondrocytes. The results of this study can be applied to other cells that require special pressure and oxygen concentration control to maintain their intrinsic properties. Additionally, this technique has the potential to be applied to the re-differentiation of cells that have lost their original properties. |
| first_indexed | 2024-03-07T18:07:49Z |
| format | Article |
| id | doaj.art-ba2a0d28bdda4f6fa7e2de9860a1517c |
| institution | Directory Open Access Journal |
| issn | 2055-7124 |
| language | English |
| last_indexed | 2024-03-07T18:07:49Z |
| publishDate | 2022-12-01 |
| publisher | American Association for the Advancement of Science (AAAS) |
| record_format | Article |
| series | Biomaterials Research |
| spelling | doaj.art-ba2a0d28bdda4f6fa7e2de9860a1517c2024-03-02T08:35:36ZengAmerican Association for the Advancement of Science (AAAS)Biomaterials Research2055-71242022-12-0126111510.1186/s40824-022-00327-wBiomimetic hydrogel blanket for conserving and recovering intrinsic cell propertiesSeung-Hoon Um0Youngmin Seo1Hyunseon Seo2Kyungwoo Lee3Sun Hwa Park4Jung Ho Jeon5Jung Yeon Lim6Myoung-Ryul Ok7Yu-Chan Kim8Hyunjung Kim9Cheol-Hong Cheon10Hyung-Seop Han11James R. Edwards12Sung Won Kim13Hojeong Jeon14Biomaterials Research Center, Biomedical Research Division, Korea Institute of Science and Technology (KIST)Biomaterials Research Center, Biomedical Research Division, Korea Institute of Science and Technology (KIST)Biomaterials Research Center, Biomedical Research Division, Korea Institute of Science and Technology (KIST)Biomaterials Research Center, Biomedical Research Division, Korea Institute of Science and Technology (KIST)Laboratory for Biomaterials and Bioengineering, Department of Min-Met-Materials Engineering, Research Center of CHU de Quebec, Division of Regenerative Medicine, Canada Research Chair I in Biomaterials and Bioengineering for the Innovation in Surgery, Laval UniversityDepartment of Otolaryngology-Head and Neck Surgery, College of Medicine, The Catholic University of KoreaDepartment of Otolaryngology-Head and Neck Surgery, College of Medicine, The Catholic University of KoreaBiomaterials Research Center, Biomedical Research Division, Korea Institute of Science and Technology (KIST)Biomaterials Research Center, Biomedical Research Division, Korea Institute of Science and Technology (KIST)Division of Nursing, Research Institute of Nursing Science, Hallym UniversityDepartment of Chemistry, Korea UniversityBiomaterials Research Center, Biomedical Research Division, Korea Institute of Science and Technology (KIST)Nuffield Department of Orthopaedics, Rheumatology and Musculoskeletal Sciences (NDORMS), Botnar Research Centre, University of OxfordDepartment of Otolaryngology-Head and Neck Surgery, College of Medicine, The Catholic University of KoreaBiomaterials Research Center, Biomedical Research Division, Korea Institute of Science and Technology (KIST)Abstract Background Cells in the human body experience different growth environments and conditions, such as compressive pressure and oxygen concentrations, depending on the type and location of the tissue. Thus, a culture device that emulates the environment inside the body is required to study cells outside the body. Methods A blanket-type cell culture device (Direct Contact Pressing: DCP) was fabricated with an alginate-based hydrogel. Changes in cell morphology due to DCP pressure were observed using a phase contrast microscope. The changes in the oxygen permeability and pressure according to the hydrogel concentration of DCP were analyzed. To compare the effects of DCP with normal or artificial hypoxic cultures, cells were divided based on the culture technique: normal culture, DCP culture device, and artificial hypoxic environment. Changes in phenotype, genes, and glycosaminoglycan amounts according to each environment were evaluated. Based on this, the mechanism of each culture environment on the intrinsic properties of conserving chondrocytes was suggested. Results Chondrocytes live under pressure from the surrounding collagen tissue and experience a hypoxic environment because collagen inhibits oxygen permeability. By culturing the chondrocytes in a DCP environment, the capability of DCP to produce a low-oxygen and physical pressure environment was verified. When human primary chondrocytes, which require pressure and a low-oxygen environment during culture to maintain their innate properties, were cultured using the hydrogel blanket, the original shapes and properties of the chondrocytes were maintained. The intrinsic properties could be recovered even in aged cells that had lost their original cell properties. Conclusions A DCP culture method using a biomimetic hydrogel blanket provides cells with an adjustable physical pressure and a low-oxygen environment. Through this technique, we could maintain the original cellular phenotypes and intrinsic properties of human primary chondrocytes. The results of this study can be applied to other cells that require special pressure and oxygen concentration control to maintain their intrinsic properties. Additionally, this technique has the potential to be applied to the re-differentiation of cells that have lost their original properties.https://doi.org/10.1186/s40824-022-00327-wBiomimeticHydrogel blanketCell culture systemPhysical stimuliConserving and recovering cell properties |
| spellingShingle | Seung-Hoon Um Youngmin Seo Hyunseon Seo Kyungwoo Lee Sun Hwa Park Jung Ho Jeon Jung Yeon Lim Myoung-Ryul Ok Yu-Chan Kim Hyunjung Kim Cheol-Hong Cheon Hyung-Seop Han James R. Edwards Sung Won Kim Hojeong Jeon Biomimetic hydrogel blanket for conserving and recovering intrinsic cell properties Biomaterials Research Biomimetic Hydrogel blanket Cell culture system Physical stimuli Conserving and recovering cell properties |
| title | Biomimetic hydrogel blanket for conserving and recovering intrinsic cell properties |
| title_full | Biomimetic hydrogel blanket for conserving and recovering intrinsic cell properties |
| title_fullStr | Biomimetic hydrogel blanket for conserving and recovering intrinsic cell properties |
| title_full_unstemmed | Biomimetic hydrogel blanket for conserving and recovering intrinsic cell properties |
| title_short | Biomimetic hydrogel blanket for conserving and recovering intrinsic cell properties |
| title_sort | biomimetic hydrogel blanket for conserving and recovering intrinsic cell properties |
| topic | Biomimetic Hydrogel blanket Cell culture system Physical stimuli Conserving and recovering cell properties |
| url | https://doi.org/10.1186/s40824-022-00327-w |
| work_keys_str_mv | AT seunghoonum biomimetichydrogelblanketforconservingandrecoveringintrinsiccellproperties AT youngminseo biomimetichydrogelblanketforconservingandrecoveringintrinsiccellproperties AT hyunseonseo biomimetichydrogelblanketforconservingandrecoveringintrinsiccellproperties AT kyungwoolee biomimetichydrogelblanketforconservingandrecoveringintrinsiccellproperties AT sunhwapark biomimetichydrogelblanketforconservingandrecoveringintrinsiccellproperties AT junghojeon biomimetichydrogelblanketforconservingandrecoveringintrinsiccellproperties AT jungyeonlim biomimetichydrogelblanketforconservingandrecoveringintrinsiccellproperties AT myoungryulok biomimetichydrogelblanketforconservingandrecoveringintrinsiccellproperties AT yuchankim biomimetichydrogelblanketforconservingandrecoveringintrinsiccellproperties AT hyunjungkim biomimetichydrogelblanketforconservingandrecoveringintrinsiccellproperties AT cheolhongcheon biomimetichydrogelblanketforconservingandrecoveringintrinsiccellproperties AT hyungseophan biomimetichydrogelblanketforconservingandrecoveringintrinsiccellproperties AT jamesredwards biomimetichydrogelblanketforconservingandrecoveringintrinsiccellproperties AT sungwonkim biomimetichydrogelblanketforconservingandrecoveringintrinsiccellproperties AT hojeongjeon biomimetichydrogelblanketforconservingandrecoveringintrinsiccellproperties |