E7 Peptide Enables BMSC Adhesion and Promotes Chondrogenic Differentiation of BMSCs Via the LncRNA H19/miR675 Axis
Therapeutic strategies based on utilizing endogenous BMSCs have been developed for the regeneration of bone, cartilage, and ligaments. We previously found that E7 peptide (EPLQLKM) could enhance BMSC homing in bio-scaffolds and, therefore, promote cartilage regeneration. However, the profile and mec...
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MDPI AG
2023-06-01
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| Series: | Bioengineering |
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| Online Access: | https://www.mdpi.com/2306-5354/10/7/781 |
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| author | Weili Shi Jiangyi Wu Yanbin Pi Xingran Yan Xiaoqing Hu Jin Cheng Huilei Yu Zhenxing Shao |
| author_facet | Weili Shi Jiangyi Wu Yanbin Pi Xingran Yan Xiaoqing Hu Jin Cheng Huilei Yu Zhenxing Shao |
| author_sort | Weili Shi |
| collection | DOAJ |
| description | Therapeutic strategies based on utilizing endogenous BMSCs have been developed for the regeneration of bone, cartilage, and ligaments. We previously found that E7 peptide (EPLQLKM) could enhance BMSC homing in bio-scaffolds and, therefore, promote cartilage regeneration. However, the profile and mechanisms of E7 peptide in cartilage regeneration remain elusive. In this study, we examined the effect of E7 peptide on the BMSC phenotype, including adhesion, viability and chondrogenic differentiation, and its underlying mechanism. The konjac glucomannan microsphere (KGM), a carrier material that is free of BMSC adhesion ability, was used as the solid base of E7 peptide to better explore the independent role of E7 peptide in BMSC behavior. The results showed that E7 peptide could support BMSC adhesion and viability in a comparable manner to RGD and promote superior chondrogenic differentiation to RGD. We examined differentially expressed genes of BMSCs induced by E7 compared to RGD. Subsequently, a real-time PCR validated the significantly upregulated expression of lncRNA H19, and the knockdown of lncRNA H19 or miR675, a downstream functional unit of H19, could significantly obscure the chondrogenic differentiation induced by E7. In conclusion, this study confirmed the independent role of E7 in the adhesion and viability of BMSCs and revealed the pro-chondrogenic effect of E7 on BMSCs via the H19/miR675 axis. These results could help establish new therapeutic strategies based on employing endogenous BMSCs for cartilage tissue regeneration. |
| first_indexed | 2024-03-11T01:17:57Z |
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| language | English |
| last_indexed | 2024-03-11T01:17:57Z |
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| spelling | doaj.art-ba2a4b6a37674351a261a0014b71b0852023-11-18T18:21:16ZengMDPI AGBioengineering2306-53542023-06-0110778110.3390/bioengineering10070781E7 Peptide Enables BMSC Adhesion and Promotes Chondrogenic Differentiation of BMSCs Via the LncRNA H19/miR675 AxisWeili Shi0Jiangyi Wu1Yanbin Pi2Xingran Yan3Xiaoqing Hu4Jin Cheng5Huilei Yu6Zhenxing Shao7Peking University Third Hospital, Beijing Key Laboratory of Sports Injuries, Department of Sports Medicine, Institute of Sports Medicine of Peking University, Beijing 100191, ChinaPlastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100144, ChinaPeking University Third Hospital, Beijing Key Laboratory of Sports Injuries, Department of Sports Medicine, Institute of Sports Medicine of Peking University, Beijing 100191, ChinaState Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, ChinaPeking University Third Hospital, Beijing Key Laboratory of Sports Injuries, Department of Sports Medicine, Institute of Sports Medicine of Peking University, Beijing 100191, ChinaPeking University Third Hospital, Beijing Key Laboratory of Sports Injuries, Department of Sports Medicine, Institute of Sports Medicine of Peking University, Beijing 100191, ChinaPeking University Third Hospital, Beijing Key Laboratory of Sports Injuries, Department of Sports Medicine, Institute of Sports Medicine of Peking University, Beijing 100191, ChinaPeking University Third Hospital, Beijing Key Laboratory of Sports Injuries, Department of Sports Medicine, Institute of Sports Medicine of Peking University, Beijing 100191, ChinaTherapeutic strategies based on utilizing endogenous BMSCs have been developed for the regeneration of bone, cartilage, and ligaments. We previously found that E7 peptide (EPLQLKM) could enhance BMSC homing in bio-scaffolds and, therefore, promote cartilage regeneration. However, the profile and mechanisms of E7 peptide in cartilage regeneration remain elusive. In this study, we examined the effect of E7 peptide on the BMSC phenotype, including adhesion, viability and chondrogenic differentiation, and its underlying mechanism. The konjac glucomannan microsphere (KGM), a carrier material that is free of BMSC adhesion ability, was used as the solid base of E7 peptide to better explore the independent role of E7 peptide in BMSC behavior. The results showed that E7 peptide could support BMSC adhesion and viability in a comparable manner to RGD and promote superior chondrogenic differentiation to RGD. We examined differentially expressed genes of BMSCs induced by E7 compared to RGD. Subsequently, a real-time PCR validated the significantly upregulated expression of lncRNA H19, and the knockdown of lncRNA H19 or miR675, a downstream functional unit of H19, could significantly obscure the chondrogenic differentiation induced by E7. In conclusion, this study confirmed the independent role of E7 in the adhesion and viability of BMSCs and revealed the pro-chondrogenic effect of E7 on BMSCs via the H19/miR675 axis. These results could help establish new therapeutic strategies based on employing endogenous BMSCs for cartilage tissue regeneration.https://www.mdpi.com/2306-5354/10/7/781mesenchymal stem cellsE7 peptidechondrogenic differentiationH19/miR-675Konjac Glucomannan microsphere |
| spellingShingle | Weili Shi Jiangyi Wu Yanbin Pi Xingran Yan Xiaoqing Hu Jin Cheng Huilei Yu Zhenxing Shao E7 Peptide Enables BMSC Adhesion and Promotes Chondrogenic Differentiation of BMSCs Via the LncRNA H19/miR675 Axis Bioengineering mesenchymal stem cells E7 peptide chondrogenic differentiation H19/miR-675 Konjac Glucomannan microsphere |
| title | E7 Peptide Enables BMSC Adhesion and Promotes Chondrogenic Differentiation of BMSCs Via the LncRNA H19/miR675 Axis |
| title_full | E7 Peptide Enables BMSC Adhesion and Promotes Chondrogenic Differentiation of BMSCs Via the LncRNA H19/miR675 Axis |
| title_fullStr | E7 Peptide Enables BMSC Adhesion and Promotes Chondrogenic Differentiation of BMSCs Via the LncRNA H19/miR675 Axis |
| title_full_unstemmed | E7 Peptide Enables BMSC Adhesion and Promotes Chondrogenic Differentiation of BMSCs Via the LncRNA H19/miR675 Axis |
| title_short | E7 Peptide Enables BMSC Adhesion and Promotes Chondrogenic Differentiation of BMSCs Via the LncRNA H19/miR675 Axis |
| title_sort | e7 peptide enables bmsc adhesion and promotes chondrogenic differentiation of bmscs via the lncrna h19 mir675 axis |
| topic | mesenchymal stem cells E7 peptide chondrogenic differentiation H19/miR-675 Konjac Glucomannan microsphere |
| url | https://www.mdpi.com/2306-5354/10/7/781 |
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