CRISPR/Cas tools for enhancing the biopreservation ability of lactic acid bacteria in aquatic products
Lactic acid bacteria (LAB) plays a crucial role in aquatic products biopreservation as it can inhibit many bacteria, in particular the specific spoilage organisms (SSOs) of aquatic products, by competing for nutrients or producing one or more metabolites which have antimicrobial activity, such as ba...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-12-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2022.1114588/full |
| _version_ | 1828088564569604096 |
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| author | Huina Dong Huiying Wang Shaoping Fu Dawei Zhang Dawei Zhang |
| author_facet | Huina Dong Huiying Wang Shaoping Fu Dawei Zhang Dawei Zhang |
| author_sort | Huina Dong |
| collection | DOAJ |
| description | Lactic acid bacteria (LAB) plays a crucial role in aquatic products biopreservation as it can inhibit many bacteria, in particular the specific spoilage organisms (SSOs) of aquatic products, by competing for nutrients or producing one or more metabolites which have antimicrobial activity, such as bacteriocins. Lactobacillus spp. and Lactococcus spp. are the most commonly used Lactic acid bacterias in aquatic products preservation. The improvement of gene editing tools is particularly important for developing new lactic acid bacteria strains with superior properties for aquatic products biopreservation. This review summarized the research progress of the most widely used CRISPR/Cas-based genome editing tools in Lactobacillus spp. and Lactococcus spp. The genome editing tools based on homologous recombination and base editor were described. Then, the research status of CRISPRi in transcriptional regulation was reviewed briefly. This review may provide a reference for the application of CRISPR/Cas-based genome editing tools to other lactic acid bacteria species. |
| first_indexed | 2024-04-11T05:27:28Z |
| format | Article |
| id | doaj.art-ba2b5c7c5c6b42879cb874fa65216e68 |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-04-11T05:27:28Z |
| publishDate | 2022-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-ba2b5c7c5c6b42879cb874fa65216e682022-12-23T05:53:56ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852022-12-011010.3389/fbioe.2022.11145881114588CRISPR/Cas tools for enhancing the biopreservation ability of lactic acid bacteria in aquatic productsHuina Dong0Huiying Wang1Shaoping Fu2Dawei Zhang3Dawei Zhang4Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, ChinaTianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, ChinaTianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, ChinaTianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaLactic acid bacteria (LAB) plays a crucial role in aquatic products biopreservation as it can inhibit many bacteria, in particular the specific spoilage organisms (SSOs) of aquatic products, by competing for nutrients or producing one or more metabolites which have antimicrobial activity, such as bacteriocins. Lactobacillus spp. and Lactococcus spp. are the most commonly used Lactic acid bacterias in aquatic products preservation. The improvement of gene editing tools is particularly important for developing new lactic acid bacteria strains with superior properties for aquatic products biopreservation. This review summarized the research progress of the most widely used CRISPR/Cas-based genome editing tools in Lactobacillus spp. and Lactococcus spp. The genome editing tools based on homologous recombination and base editor were described. Then, the research status of CRISPRi in transcriptional regulation was reviewed briefly. This review may provide a reference for the application of CRISPR/Cas-based genome editing tools to other lactic acid bacteria species.https://www.frontiersin.org/articles/10.3389/fbioe.2022.1114588/fulllactic acid bacteriaaquatic products biopreservationCRISPR/Cas systemgenome editingtranscriptional regulation |
| spellingShingle | Huina Dong Huiying Wang Shaoping Fu Dawei Zhang Dawei Zhang CRISPR/Cas tools for enhancing the biopreservation ability of lactic acid bacteria in aquatic products Frontiers in Bioengineering and Biotechnology lactic acid bacteria aquatic products biopreservation CRISPR/Cas system genome editing transcriptional regulation |
| title | CRISPR/Cas tools for enhancing the biopreservation ability of lactic acid bacteria in aquatic products |
| title_full | CRISPR/Cas tools for enhancing the biopreservation ability of lactic acid bacteria in aquatic products |
| title_fullStr | CRISPR/Cas tools for enhancing the biopreservation ability of lactic acid bacteria in aquatic products |
| title_full_unstemmed | CRISPR/Cas tools for enhancing the biopreservation ability of lactic acid bacteria in aquatic products |
| title_short | CRISPR/Cas tools for enhancing the biopreservation ability of lactic acid bacteria in aquatic products |
| title_sort | crispr cas tools for enhancing the biopreservation ability of lactic acid bacteria in aquatic products |
| topic | lactic acid bacteria aquatic products biopreservation CRISPR/Cas system genome editing transcriptional regulation |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2022.1114588/full |
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