Cytotoxic Effects of Eupatorium cannabinum on MCF-7 Human Breast Cancer Cell Line
Background and objectives: Cancer is known to be the second cause of death around the world. The most prevalent cancer among women is breast cancer. Use of plant-derived products in cancer treatment may reduce adverse side effects. Extensive research around the world has led to the discovery of herb...
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Iranian Society of Pharmacognosy
2021-04-01
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| Series: | Research Journal of Pharmacognosy |
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| Online Access: | http://www.rjpharmacognosy.ir/article_128149_1d865e1ff2300a9cef3e2abd1e4bd873.pdf |
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| author | Morteza Abuali Mohammad Reza Shams Ardekani* Hassan Rezadoost Mahdi Vazirian Majid Balaei-Kahnamoei Maryam Hamzeloo-Moghadam |
| author_facet | Morteza Abuali Mohammad Reza Shams Ardekani* Hassan Rezadoost Mahdi Vazirian Majid Balaei-Kahnamoei Maryam Hamzeloo-Moghadam |
| author_sort | Morteza Abuali |
| collection | DOAJ |
| description | Background and objectives: Cancer is known to be the second cause of death around the world. The most prevalent cancer among women is breast cancer. Use of plant-derived products in cancer treatment may reduce adverse side effects. Extensive research around the world has led to the discovery of herbal compounds that can be used to treat some types of cancer. According to previous studies, the methanol extract of Eupatorium cannabinum has shown cytotoxic effects in some cancer cell lines. In the present study, bioassay guided fractionation and isolation was conducted on E. cannabinum to evaluate the cytotoxic activityin MCF-7 breast cancer cell line. Methods: The Extraction from theaerial parts was performed by maceration method. Isolation and purification of the extracts were performed by column chromatography. The cytotoxic activity of different extracts of E. cannabinum was evaluated against MCF-7 cell line by MTT assay and a compound was isolated according to bioassay guided fractionation. The cytotoxic activity and apoptotic property of the isolated compound was determined. Results: The chloroform extract was the most active one with IC50 of 21.39±3.24 μg/mL followed by the n-hexane and methanol extracts with IC50values of 60.23±2.16 μg/mL and 81.74±3.41 μg/mL, respectively. IC50 of subfractions (1-6) from the chloroform extract were 60.83±2.56 μg/mL, 58.93±2.73 μg/mL, 37.5±3.65 μg/mL, 7.86±1.34 μg/mL, 10.61±2.34 μg/mL and 13.77±4.17 μg/mL, respectively. Eucannabinolide, a sesquiterpene lactone, was isolated from the chloroform extract according to bioassay guided fractionation. Its IC50 was found to be 13±2.45 μg/mL. Eucannabinolide induced 46.91% apoptosis at concentration of 13 μg/mL in MCF-7 cell line in Annexin V/PI assay. Conclusion: Eucannabinolideis a promising candidate for further breast cancer drug discovery studies. |
| first_indexed | 2024-12-24T01:33:20Z |
| format | Article |
| id | doaj.art-ba2d03f66ef843d7833ff47b94afdc7f |
| institution | Directory Open Access Journal |
| issn | 2345-4458 2345-5977 |
| language | English |
| last_indexed | 2024-12-24T01:33:20Z |
| publishDate | 2021-04-01 |
| publisher | Iranian Society of Pharmacognosy |
| record_format | Article |
| series | Research Journal of Pharmacognosy |
| spelling | doaj.art-ba2d03f66ef843d7833ff47b94afdc7f2022-12-21T17:22:16ZengIranian Society of PharmacognosyResearch Journal of Pharmacognosy2345-44582345-59772021-04-0182697510.22127/rjp.2021.263706.1654128149Cytotoxic Effects of Eupatorium cannabinum on MCF-7 Human Breast Cancer Cell LineMorteza Abuali0Mohammad Reza Shams Ardekani*1Hassan Rezadoost2Mahdi Vazirian3Majid Balaei-Kahnamoei4Maryam Hamzeloo-Moghadam5Department of Pharmacognosy, Faculty of Pharmacy, Tehran, University of Medical Sciences, Tehran, Iran.Department of Pharmacognosy, Faculty of Pharmacy, Tehran, University of Medical Sciences, Tehran, Iran.Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran, Iran.Department of Pharmacognosy, Faculty of Pharmacy, Tehran, University of Medical Sciences, Tehran, Iran.Department of Pharmacognosy, Faculty of Pharmacy, Tehran, University of Medical Sciences, Tehran, Iran.Traditional Medicine and Materia Medica Research Center and Department of Traditional Pharmacy, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.Background and objectives: Cancer is known to be the second cause of death around the world. The most prevalent cancer among women is breast cancer. Use of plant-derived products in cancer treatment may reduce adverse side effects. Extensive research around the world has led to the discovery of herbal compounds that can be used to treat some types of cancer. According to previous studies, the methanol extract of Eupatorium cannabinum has shown cytotoxic effects in some cancer cell lines. In the present study, bioassay guided fractionation and isolation was conducted on E. cannabinum to evaluate the cytotoxic activityin MCF-7 breast cancer cell line. Methods: The Extraction from theaerial parts was performed by maceration method. Isolation and purification of the extracts were performed by column chromatography. The cytotoxic activity of different extracts of E. cannabinum was evaluated against MCF-7 cell line by MTT assay and a compound was isolated according to bioassay guided fractionation. The cytotoxic activity and apoptotic property of the isolated compound was determined. Results: The chloroform extract was the most active one with IC50 of 21.39±3.24 μg/mL followed by the n-hexane and methanol extracts with IC50values of 60.23±2.16 μg/mL and 81.74±3.41 μg/mL, respectively. IC50 of subfractions (1-6) from the chloroform extract were 60.83±2.56 μg/mL, 58.93±2.73 μg/mL, 37.5±3.65 μg/mL, 7.86±1.34 μg/mL, 10.61±2.34 μg/mL and 13.77±4.17 μg/mL, respectively. Eucannabinolide, a sesquiterpene lactone, was isolated from the chloroform extract according to bioassay guided fractionation. Its IC50 was found to be 13±2.45 μg/mL. Eucannabinolide induced 46.91% apoptosis at concentration of 13 μg/mL in MCF-7 cell line in Annexin V/PI assay. Conclusion: Eucannabinolideis a promising candidate for further breast cancer drug discovery studies.http://www.rjpharmacognosy.ir/article_128149_1d865e1ff2300a9cef3e2abd1e4bd873.pdfapoptosiscytotoxicityeupatorium cannabinumeucannabinolidemcf-7 |
| spellingShingle | Morteza Abuali Mohammad Reza Shams Ardekani* Hassan Rezadoost Mahdi Vazirian Majid Balaei-Kahnamoei Maryam Hamzeloo-Moghadam Cytotoxic Effects of Eupatorium cannabinum on MCF-7 Human Breast Cancer Cell Line Research Journal of Pharmacognosy apoptosis cytotoxicity eupatorium cannabinum eucannabinolide mcf-7 |
| title | Cytotoxic Effects of Eupatorium cannabinum on MCF-7 Human Breast Cancer Cell Line |
| title_full | Cytotoxic Effects of Eupatorium cannabinum on MCF-7 Human Breast Cancer Cell Line |
| title_fullStr | Cytotoxic Effects of Eupatorium cannabinum on MCF-7 Human Breast Cancer Cell Line |
| title_full_unstemmed | Cytotoxic Effects of Eupatorium cannabinum on MCF-7 Human Breast Cancer Cell Line |
| title_short | Cytotoxic Effects of Eupatorium cannabinum on MCF-7 Human Breast Cancer Cell Line |
| title_sort | cytotoxic effects of eupatorium cannabinum on mcf 7 human breast cancer cell line |
| topic | apoptosis cytotoxicity eupatorium cannabinum eucannabinolide mcf-7 |
| url | http://www.rjpharmacognosy.ir/article_128149_1d865e1ff2300a9cef3e2abd1e4bd873.pdf |
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