Determination of 3-nitropropionic acid in plasma and urine by ion chromatography-triple quadrupole mass spectrometry
Objective A simple and sensitive method for the determination of 3-nitropropionic acid (3-NPA) in plasma and urine by ion chromatography-triple quadrupole mass spectrometry (IC-MS/MS) was established. Methods Plasma and urine samples were extracted with aqueous solution containing 3% perchloric acid...
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| Format: | Article |
| Language: | zho |
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The Editorial Office of Chinese Journal of Food Hygiene
2020-03-01
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| Series: | Zhongguo shipin weisheng zazhi |
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| Online Access: | http://www.zgspws.com/zgspwszzen/article/abstract/20200208 |
| _version_ | 1797769054742642688 |
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| author | Xiaoyi ZHANG Xiuyao ZHANG Xinxin CAI Ruifen LI |
| author_facet | Xiaoyi ZHANG Xiuyao ZHANG Xinxin CAI Ruifen LI |
| author_sort | Xiaoyi ZHANG |
| collection | DOAJ |
| description | Objective A simple and sensitive method for the determination of 3-nitropropionic acid (3-NPA) in plasma and urine by ion chromatography-triple quadrupole mass spectrometry (IC-MS/MS) was established. Methods Plasma and urine samples were extracted with aqueous solution containing 3% perchloric acid, and then the extract was centrifuged to remove the protein and lipids. The target analyte in the extract was cleaned-up by solid supported liquid/liquid extraction under pH=1-2, and methyl tert-butyl ether (MTBE) was used as eluent. After the MTBE was removed by nitrogen, the 3-NPA in the residues was dissolved into water. The separation of 3-NPA was carried out on a Dionex Ionpac AS19 analytic column (250 mm×2 mm, 7.5 μm) with gradient elution using KOH solution electrolytically generated from on-line eluent generation cartridge. Before the eluent flow entered the ion source of mass spectrometer, the potassium ion in the eluent was removed by an in-line suppressor. The 3-NPA was detected by negative electrospray ionization triple quadrupole mass spectrometry in the multiple reaction monitoring (MRM) mode. Results The result showed that the correlation coefficients of linear calibration cuve of 3-NPA were above 0.999 at the corresponding concentration ranges of 0.1-250 μg/L. The average recoveries were 92.0%-114% for 3-NPA in plasma and urine with relative standard deviations of 2.0%-12% (n=6). The limits of detection of 3-NPA (S/N=3) were 0.1 μg/L. Conclusion The method is simple, sensitive and accurate, and can be used for the determination of 3-NPA in plasma and urine. |
| first_indexed | 2024-03-12T21:03:18Z |
| format | Article |
| id | doaj.art-ba2d5e3f7c8e4328be0eb9829aa1756a |
| institution | Directory Open Access Journal |
| issn | 1004-8456 |
| language | zho |
| last_indexed | 2024-03-12T21:03:18Z |
| publishDate | 2020-03-01 |
| publisher | The Editorial Office of Chinese Journal of Food Hygiene |
| record_format | Article |
| series | Zhongguo shipin weisheng zazhi |
| spelling | doaj.art-ba2d5e3f7c8e4328be0eb9829aa1756a2023-07-31T02:31:26ZzhoThe Editorial Office of Chinese Journal of Food HygieneZhongguo shipin weisheng zazhi1004-84562020-03-01320214915410.13590/j.cjfh.2020.02.0081004-8456(2020)02-0149-06Determination of 3-nitropropionic acid in plasma and urine by ion chromatography-triple quadrupole mass spectrometryXiaoyi ZHANGXiuyao ZHANGXinxin CAIRuifen LIObjective A simple and sensitive method for the determination of 3-nitropropionic acid (3-NPA) in plasma and urine by ion chromatography-triple quadrupole mass spectrometry (IC-MS/MS) was established. Methods Plasma and urine samples were extracted with aqueous solution containing 3% perchloric acid, and then the extract was centrifuged to remove the protein and lipids. The target analyte in the extract was cleaned-up by solid supported liquid/liquid extraction under pH=1-2, and methyl tert-butyl ether (MTBE) was used as eluent. After the MTBE was removed by nitrogen, the 3-NPA in the residues was dissolved into water. The separation of 3-NPA was carried out on a Dionex Ionpac AS19 analytic column (250 mm×2 mm, 7.5 μm) with gradient elution using KOH solution electrolytically generated from on-line eluent generation cartridge. Before the eluent flow entered the ion source of mass spectrometer, the potassium ion in the eluent was removed by an in-line suppressor. The 3-NPA was detected by negative electrospray ionization triple quadrupole mass spectrometry in the multiple reaction monitoring (MRM) mode. Results The result showed that the correlation coefficients of linear calibration cuve of 3-NPA were above 0.999 at the corresponding concentration ranges of 0.1-250 μg/L. The average recoveries were 92.0%-114% for 3-NPA in plasma and urine with relative standard deviations of 2.0%-12% (n=6). The limits of detection of 3-NPA (S/N=3) were 0.1 μg/L. Conclusion The method is simple, sensitive and accurate, and can be used for the determination of 3-NPA in plasma and urine.http://www.zgspws.com/zgspwszzen/article/abstract/20200208ion chromatography-triple quadrupole mass spectrometrybiological samplesplasmaurine3-nitropropionic acid |
| spellingShingle | Xiaoyi ZHANG Xiuyao ZHANG Xinxin CAI Ruifen LI Determination of 3-nitropropionic acid in plasma and urine by ion chromatography-triple quadrupole mass spectrometry Zhongguo shipin weisheng zazhi ion chromatography-triple quadrupole mass spectrometry biological samples plasma urine 3-nitropropionic acid |
| title | Determination of 3-nitropropionic acid in plasma and urine by ion chromatography-triple quadrupole mass spectrometry |
| title_full | Determination of 3-nitropropionic acid in plasma and urine by ion chromatography-triple quadrupole mass spectrometry |
| title_fullStr | Determination of 3-nitropropionic acid in plasma and urine by ion chromatography-triple quadrupole mass spectrometry |
| title_full_unstemmed | Determination of 3-nitropropionic acid in plasma and urine by ion chromatography-triple quadrupole mass spectrometry |
| title_short | Determination of 3-nitropropionic acid in plasma and urine by ion chromatography-triple quadrupole mass spectrometry |
| title_sort | determination of 3 nitropropionic acid in plasma and urine by ion chromatography triple quadrupole mass spectrometry |
| topic | ion chromatography-triple quadrupole mass spectrometry biological samples plasma urine 3-nitropropionic acid |
| url | http://www.zgspws.com/zgspwszzen/article/abstract/20200208 |
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