Lipidomics and RNA-Seq Study of Lipid Regulation in Aphis gossypii parasitized by Lysiphlebia japonica
Abstract The cotton–melon aphid, Aphis gossypii Glover, is a major insect pest worldwide. Lysiphlebia japonica (Ashmead) is an obligate parasitic wasp of A. gossypii, and has the ability to regulate lipid metabolism of the cotton-melon aphid. Lipids are known to play critical roles in energy homeost...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2017-05-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-017-01546-1 |
| _version_ | 1818427688571895808 |
|---|---|
| author | Gao XueKe Zhang Shuai Luo JunYu Lü LiMin Zhang LiJuan Cui JinJie |
| author_facet | Gao XueKe Zhang Shuai Luo JunYu Lü LiMin Zhang LiJuan Cui JinJie |
| author_sort | Gao XueKe |
| collection | DOAJ |
| description | Abstract The cotton–melon aphid, Aphis gossypii Glover, is a major insect pest worldwide. Lysiphlebia japonica (Ashmead) is an obligate parasitic wasp of A. gossypii, and has the ability to regulate lipid metabolism of the cotton-melon aphid. Lipids are known to play critical roles in energy homeostasis, membrane structure, and signaling. However, the parasitoid genes that regulate fat metabolism and lipid composition in aphids are not known. 34 glycerolipids and 248 glycerophospholipids were identified in this study. We have shown that a 3-day parasitism of aphids can induce significant changes in the content and acyl chain composition of triacylglycerols (TAGs) and subspecies composition of glycerophospholipids content and acyl chains. It also upregulate the expression of several genes involved in triacylglycerol synthesis and glycerophospholipid metabolism. Pathway analysis showed that a higher expression of genes involved in the tricarboxylic acid cycle and glycolysis pathways may contribute to TAGs synthesis in parasitized aphids. Interestingly, the higher expression of genes in the sphingomyelin pathway and reduced sphingomyelin content may be related to the reproductive ability of A. gossypii. We provide a comprehensive resource describing the molecular signature of parasitized A. gossypii particularly the changes associated with the lipid metabolism and discuss the biological and ecological significance of this change. |
| first_indexed | 2024-12-14T14:49:42Z |
| format | Article |
| id | doaj.art-ba2ea52e6a3740cd8f967cf557a4958b |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-14T14:49:42Z |
| publishDate | 2017-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-ba2ea52e6a3740cd8f967cf557a4958b2022-12-21T22:57:11ZengNature PortfolioScientific Reports2045-23222017-05-017111310.1038/s41598-017-01546-1Lipidomics and RNA-Seq Study of Lipid Regulation in Aphis gossypii parasitized by Lysiphlebia japonicaGao XueKe0Zhang Shuai1Luo JunYu2Lü LiMin3Zhang LiJuan4Cui JinJie5Institute of Cotton Research, Chinese Academy of Agricultural Sciences/State Key Laboratory of Cotton BiologyInstitute of Cotton Research, Chinese Academy of Agricultural Sciences/State Key Laboratory of Cotton BiologyInstitute of Cotton Research, Chinese Academy of Agricultural Sciences/State Key Laboratory of Cotton BiologyInstitute of Cotton Research, Chinese Academy of Agricultural Sciences/State Key Laboratory of Cotton BiologyInstitute of Cotton Research, Chinese Academy of Agricultural Sciences/State Key Laboratory of Cotton BiologyInstitute of Cotton Research, Chinese Academy of Agricultural Sciences/State Key Laboratory of Cotton BiologyAbstract The cotton–melon aphid, Aphis gossypii Glover, is a major insect pest worldwide. Lysiphlebia japonica (Ashmead) is an obligate parasitic wasp of A. gossypii, and has the ability to regulate lipid metabolism of the cotton-melon aphid. Lipids are known to play critical roles in energy homeostasis, membrane structure, and signaling. However, the parasitoid genes that regulate fat metabolism and lipid composition in aphids are not known. 34 glycerolipids and 248 glycerophospholipids were identified in this study. We have shown that a 3-day parasitism of aphids can induce significant changes in the content and acyl chain composition of triacylglycerols (TAGs) and subspecies composition of glycerophospholipids content and acyl chains. It also upregulate the expression of several genes involved in triacylglycerol synthesis and glycerophospholipid metabolism. Pathway analysis showed that a higher expression of genes involved in the tricarboxylic acid cycle and glycolysis pathways may contribute to TAGs synthesis in parasitized aphids. Interestingly, the higher expression of genes in the sphingomyelin pathway and reduced sphingomyelin content may be related to the reproductive ability of A. gossypii. We provide a comprehensive resource describing the molecular signature of parasitized A. gossypii particularly the changes associated with the lipid metabolism and discuss the biological and ecological significance of this change.https://doi.org/10.1038/s41598-017-01546-1 |
| spellingShingle | Gao XueKe Zhang Shuai Luo JunYu Lü LiMin Zhang LiJuan Cui JinJie Lipidomics and RNA-Seq Study of Lipid Regulation in Aphis gossypii parasitized by Lysiphlebia japonica Scientific Reports |
| title | Lipidomics and RNA-Seq Study of Lipid Regulation in Aphis gossypii parasitized by Lysiphlebia japonica |
| title_full | Lipidomics and RNA-Seq Study of Lipid Regulation in Aphis gossypii parasitized by Lysiphlebia japonica |
| title_fullStr | Lipidomics and RNA-Seq Study of Lipid Regulation in Aphis gossypii parasitized by Lysiphlebia japonica |
| title_full_unstemmed | Lipidomics and RNA-Seq Study of Lipid Regulation in Aphis gossypii parasitized by Lysiphlebia japonica |
| title_short | Lipidomics and RNA-Seq Study of Lipid Regulation in Aphis gossypii parasitized by Lysiphlebia japonica |
| title_sort | lipidomics and rna seq study of lipid regulation in aphis gossypii parasitized by lysiphlebia japonica |
| url | https://doi.org/10.1038/s41598-017-01546-1 |
| work_keys_str_mv | AT gaoxueke lipidomicsandrnaseqstudyoflipidregulationinaphisgossypiiparasitizedbylysiphlebiajaponica AT zhangshuai lipidomicsandrnaseqstudyoflipidregulationinaphisgossypiiparasitizedbylysiphlebiajaponica AT luojunyu lipidomicsandrnaseqstudyoflipidregulationinaphisgossypiiparasitizedbylysiphlebiajaponica AT lulimin lipidomicsandrnaseqstudyoflipidregulationinaphisgossypiiparasitizedbylysiphlebiajaponica AT zhanglijuan lipidomicsandrnaseqstudyoflipidregulationinaphisgossypiiparasitizedbylysiphlebiajaponica AT cuijinjie lipidomicsandrnaseqstudyoflipidregulationinaphisgossypiiparasitizedbylysiphlebiajaponica |