Preparation and process optimization of microbial organic copper as a feed additive
ABSTRACT As an essential trace element for animals, copper significantly contributes to the growth and health of animals. Compared to inorganic trace elements, organic trace elements are better supplements; notably, they are acquired through microbial transformation. Therefore, we screened for coppe...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Universidade Federal de Minas Gerais
2021-11-01
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| Series: | Arquivo Brasileiro de Medicina Veterinária e Zootecnia |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352021000601225&tlng=en |
| _version_ | 1830190806204416000 |
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| author | Y.R. Wei G.X. Wei Z.Y. Wang D.D. Xie X.Y. Fan Z.P. Jia J. Zhang X.G. Zhang |
| author_facet | Y.R. Wei G.X. Wei Z.Y. Wang D.D. Xie X.Y. Fan Z.P. Jia J. Zhang X.G. Zhang |
| author_sort | Y.R. Wei |
| collection | DOAJ |
| description | ABSTRACT As an essential trace element for animals, copper significantly contributes to the growth and health of animals. Compared to inorganic trace elements, organic trace elements are better supplements; notably, they are acquired through microbial transformation. Therefore, we screened for copper-enriched microorganisms from high copper content soil to obtain organic copper. Sodium diethyldithio carbamate trihydrate was applied as a chromogenic agent for determining micro amounts of intracellular copper through spectrophotometry. In total, 50 fungi were isolated after the successful application of the screening platform for copper-rich microbes. Following morphological and molecular biology analyses, the N-2 strain, identified as Aspergillus niger sp. demonstrated showed better copper enrichment potential than others. Notably, the strain tolerance to copper was nearly thrice that of Saccharomyces cerevisiae, up to 1600mg/L. The content of the organic bound copper was 22.84mg Cu/g dry cell. Using the Central Composite Design (CCD) response surface method, we optimized the fermentation condition (inoculation amount, 13%; temperature, 28(C; pH, 5.0). Compared to the original strain results under the single factor fermentation condition, we reported an increase by 24.18% under the optimized conditions. Collectively, these findings provide a reference for uncovering new and low-cost organic copper additives. |
| first_indexed | 2024-12-17T23:27:54Z |
| format | Article |
| id | doaj.art-ba2efafad2c54adf84623b347f030f00 |
| institution | Directory Open Access Journal |
| issn | 1678-4162 |
| language | English |
| last_indexed | 2024-12-17T23:27:54Z |
| publishDate | 2021-11-01 |
| publisher | Universidade Federal de Minas Gerais |
| record_format | Article |
| series | Arquivo Brasileiro de Medicina Veterinária e Zootecnia |
| spelling | doaj.art-ba2efafad2c54adf84623b347f030f002022-12-21T21:28:44ZengUniversidade Federal de Minas GeraisArquivo Brasileiro de Medicina Veterinária e Zootecnia1678-41622021-11-017351225123610.1590/1678-4162-12216Preparation and process optimization of microbial organic copper as a feed additiveY.R. WeiG.X. WeiZ.Y. WangD.D. XieX.Y. FanZ.P. JiaJ. ZhangX.G. Zhanghttps://orcid.org/0000-0002-7266-2471ABSTRACT As an essential trace element for animals, copper significantly contributes to the growth and health of animals. Compared to inorganic trace elements, organic trace elements are better supplements; notably, they are acquired through microbial transformation. Therefore, we screened for copper-enriched microorganisms from high copper content soil to obtain organic copper. Sodium diethyldithio carbamate trihydrate was applied as a chromogenic agent for determining micro amounts of intracellular copper through spectrophotometry. In total, 50 fungi were isolated after the successful application of the screening platform for copper-rich microbes. Following morphological and molecular biology analyses, the N-2 strain, identified as Aspergillus niger sp. demonstrated showed better copper enrichment potential than others. Notably, the strain tolerance to copper was nearly thrice that of Saccharomyces cerevisiae, up to 1600mg/L. The content of the organic bound copper was 22.84mg Cu/g dry cell. Using the Central Composite Design (CCD) response surface method, we optimized the fermentation condition (inoculation amount, 13%; temperature, 28(C; pH, 5.0). Compared to the original strain results under the single factor fermentation condition, we reported an increase by 24.18% under the optimized conditions. Collectively, these findings provide a reference for uncovering new and low-cost organic copper additives.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352021000601225&tlng=enorganic copperscreeningidentificationprocess optimization |
| spellingShingle | Y.R. Wei G.X. Wei Z.Y. Wang D.D. Xie X.Y. Fan Z.P. Jia J. Zhang X.G. Zhang Preparation and process optimization of microbial organic copper as a feed additive Arquivo Brasileiro de Medicina Veterinária e Zootecnia organic copper screening identification process optimization |
| title | Preparation and process optimization of microbial organic copper as a feed additive |
| title_full | Preparation and process optimization of microbial organic copper as a feed additive |
| title_fullStr | Preparation and process optimization of microbial organic copper as a feed additive |
| title_full_unstemmed | Preparation and process optimization of microbial organic copper as a feed additive |
| title_short | Preparation and process optimization of microbial organic copper as a feed additive |
| title_sort | preparation and process optimization of microbial organic copper as a feed additive |
| topic | organic copper screening identification process optimization |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352021000601225&tlng=en |
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