Molecular and cytogenetic analyses of cryptic species within the Synbranchus marmoratus Bloch, 1795 (Synbranchiformes: Synbranchidae) grouping: species delimitations, karyotypic evolution and intraspecific diversification
The fish species Synbranchus marmoratushas been reported to exist as a species complex due to high intraspecific karyotypic variability in spite of the difficulty or impossibility to distinguish them using morphological traits alone. The goal of this work was to use cytogenetic and molecular methods...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Ictiologia
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| Series: | Neotropical Ichthyology |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252014000400903&lng=en&tlng=en |
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| author | Ricardo Utsunomia José C. Pansonato-Alves Guilherme J. Costa-Silva Fernando F. Mendonça Priscilla C. Scacchetti Claudio Oliveira Fausto Foresti |
| author_facet | Ricardo Utsunomia José C. Pansonato-Alves Guilherme J. Costa-Silva Fernando F. Mendonça Priscilla C. Scacchetti Claudio Oliveira Fausto Foresti |
| author_sort | Ricardo Utsunomia |
| collection | DOAJ |
| description | The fish species Synbranchus marmoratushas been reported to exist as a species complex due to high intraspecific karyotypic variability in spite of the difficulty or impossibility to distinguish them using morphological traits alone. The goal of this work was to use cytogenetic and molecular methods to determine the species delimitations and understand the karyoevolution ofS. marmoratususing samples collected from distinct Brazilian localities. Among the analyzed specimens, a large degree of cytogenetic variation related to diploid numbers and karyotype structure was observed, with karyotypes showing 2n=42, 44 and 46 chromosomes. In addition, using sequences of three mitochondrial genes, the phylogenetic relationships between every sample with a known karyotype were determined, which revealed significant nucleotide divergence among the karyomorphs. Also, the analyses indicate that chromosomal rearrangements occurred independently within the distinct lineages of S. marmoratuscomplex, which resulted in the appearance of distinct karyotypic variants in a non-linear fashion related to diploid numbers and in the appearance of similar non-homologous chromosomes. Finally, the integration of both molecular cytogenetic and phylogenetic approaches allowed the determination of specific chromosomes possibly involved in rearrangements and a better understanding about the evolutionary processes involved in the differentiation ofSynbranchusgenus. |
| first_indexed | 2024-12-20T14:16:46Z |
| format | Article |
| id | doaj.art-ba308532662345d69624680783376a36 |
| institution | Directory Open Access Journal |
| issn | 1982-0224 |
| language | English |
| last_indexed | 2024-12-20T14:16:46Z |
| publisher | Sociedade Brasileira de Ictiologia |
| record_format | Article |
| series | Neotropical Ichthyology |
| spelling | doaj.art-ba308532662345d69624680783376a362022-12-21T19:38:01ZengSociedade Brasileira de IctiologiaNeotropical Ichthyology1982-022412490391110.1590/1982-0224-20140039S1679-62252014000400903Molecular and cytogenetic analyses of cryptic species within the Synbranchus marmoratus Bloch, 1795 (Synbranchiformes: Synbranchidae) grouping: species delimitations, karyotypic evolution and intraspecific diversificationRicardo UtsunomiaJosé C. Pansonato-AlvesGuilherme J. Costa-SilvaFernando F. MendonçaPriscilla C. ScacchettiClaudio OliveiraFausto ForestiThe fish species Synbranchus marmoratushas been reported to exist as a species complex due to high intraspecific karyotypic variability in spite of the difficulty or impossibility to distinguish them using morphological traits alone. The goal of this work was to use cytogenetic and molecular methods to determine the species delimitations and understand the karyoevolution ofS. marmoratususing samples collected from distinct Brazilian localities. Among the analyzed specimens, a large degree of cytogenetic variation related to diploid numbers and karyotype structure was observed, with karyotypes showing 2n=42, 44 and 46 chromosomes. In addition, using sequences of three mitochondrial genes, the phylogenetic relationships between every sample with a known karyotype were determined, which revealed significant nucleotide divergence among the karyomorphs. Also, the analyses indicate that chromosomal rearrangements occurred independently within the distinct lineages of S. marmoratuscomplex, which resulted in the appearance of distinct karyotypic variants in a non-linear fashion related to diploid numbers and in the appearance of similar non-homologous chromosomes. Finally, the integration of both molecular cytogenetic and phylogenetic approaches allowed the determination of specific chromosomes possibly involved in rearrangements and a better understanding about the evolutionary processes involved in the differentiation ofSynbranchusgenus.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252014000400903&lng=en&tlng=enChromosomal diversityFish cytogeneticskaryotype evolutionRepetitive DNASpecies complex |
| spellingShingle | Ricardo Utsunomia José C. Pansonato-Alves Guilherme J. Costa-Silva Fernando F. Mendonça Priscilla C. Scacchetti Claudio Oliveira Fausto Foresti Molecular and cytogenetic analyses of cryptic species within the Synbranchus marmoratus Bloch, 1795 (Synbranchiformes: Synbranchidae) grouping: species delimitations, karyotypic evolution and intraspecific diversification Neotropical Ichthyology Chromosomal diversity Fish cytogenetics karyotype evolution Repetitive DNA Species complex |
| title | Molecular and cytogenetic analyses of cryptic species within the Synbranchus marmoratus Bloch, 1795 (Synbranchiformes: Synbranchidae) grouping: species delimitations, karyotypic evolution and intraspecific diversification |
| title_full | Molecular and cytogenetic analyses of cryptic species within the Synbranchus marmoratus Bloch, 1795 (Synbranchiformes: Synbranchidae) grouping: species delimitations, karyotypic evolution and intraspecific diversification |
| title_fullStr | Molecular and cytogenetic analyses of cryptic species within the Synbranchus marmoratus Bloch, 1795 (Synbranchiformes: Synbranchidae) grouping: species delimitations, karyotypic evolution and intraspecific diversification |
| title_full_unstemmed | Molecular and cytogenetic analyses of cryptic species within the Synbranchus marmoratus Bloch, 1795 (Synbranchiformes: Synbranchidae) grouping: species delimitations, karyotypic evolution and intraspecific diversification |
| title_short | Molecular and cytogenetic analyses of cryptic species within the Synbranchus marmoratus Bloch, 1795 (Synbranchiformes: Synbranchidae) grouping: species delimitations, karyotypic evolution and intraspecific diversification |
| title_sort | molecular and cytogenetic analyses of cryptic species within the synbranchus marmoratus bloch 1795 synbranchiformes synbranchidae grouping species delimitations karyotypic evolution and intraspecific diversification |
| topic | Chromosomal diversity Fish cytogenetics karyotype evolution Repetitive DNA Species complex |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252014000400903&lng=en&tlng=en |
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