Plasmid Composition, Antimicrobial Resistance and Virulence Genes Profiles of Ciprofloxacin- and Third-Generation Cephalosporin-Resistant Foodborne <i>Salmonella enterica</i> Isolates from Russia
<i>Salmonella enterica</i> is an important foodborne pathogen worldwide. Ciprofloxacin and extended-spectrum cephalosporins are the common first-line antimicrobial drugs for the treatment of salmonellosis, antimicrobial resistance genes for which are mostly transferred via plasmids. The...
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MDPI AG
2023-01-01
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| author | Anna Egorova Andrey Shelenkov Konstantin Kuleshov Nina Kulikova Aleksey Chernyshkov Igor Manzeniuk Yulia Mikhaylova Vasiliy Akimkin |
| author_facet | Anna Egorova Andrey Shelenkov Konstantin Kuleshov Nina Kulikova Aleksey Chernyshkov Igor Manzeniuk Yulia Mikhaylova Vasiliy Akimkin |
| author_sort | Anna Egorova |
| collection | DOAJ |
| description | <i>Salmonella enterica</i> is an important foodborne pathogen worldwide. Ciprofloxacin and extended-spectrum cephalosporins are the common first-line antimicrobial drugs for the treatment of salmonellosis, antimicrobial resistance genes for which are mostly transferred via plasmids. The goal of this work was to perform genomic analysis of plasmids from foodborne <i>S. enterica</i> isolates obtained in Russia based on whole-genome sequencing. In the current study, 11 multidrug-resistant samples isolated in 2021 from 8 regions of Russia were selected based on their resistance to ciprofloxacin and third-generation cephalosporins (CIP-3rd). Whole-genome short-read sequencing (WGS) was performed for all isolates; the samples belonged to five different sequence types (ST32, ST469, ST11, ST142, and ST548) which had different profiles of antimicrobial resistance (AMR) and virulence genes. We have performed additional long-read sequencing of four representative <i>S. enterica</i> isolates, which showed that they carried pESI-like megaplasmids of 202–280 kb length harboring extended-spectrum β-lactamase genes, fluoroquinolone, tetracycline, and aminoglycosides resistance genes, as well as several virulence determinants. We believe that the WGS data obtained will greatly facilitate further studies of foodborne <i>S. enterica</i> isolates epidemiology in terms of their self-transmissible plasmid composition that mediated antimicrobial resistance and virulence determinants conferring selective advantages of this important bacterial pathogen. |
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| spelling | doaj.art-ba37e5e4eaeb4d8fb95ce4b29a0d6c202023-11-16T22:14:37ZengMDPI AGMicroorganisms2076-26072023-01-0111234710.3390/microorganisms11020347Plasmid Composition, Antimicrobial Resistance and Virulence Genes Profiles of Ciprofloxacin- and Third-Generation Cephalosporin-Resistant Foodborne <i>Salmonella enterica</i> Isolates from RussiaAnna Egorova0Andrey Shelenkov1Konstantin Kuleshov2Nina Kulikova3Aleksey Chernyshkov4Igor Manzeniuk5Yulia Mikhaylova6Vasiliy Akimkin7Central Research Institute of Epidemiology, Novogireevskaya Str., 3a, 111123 Moscow, RussiaCentral Research Institute of Epidemiology, Novogireevskaya Str., 3a, 111123 Moscow, RussiaCentral Research Institute of Epidemiology, Novogireevskaya Str., 3a, 111123 Moscow, RussiaCentral Research Institute of Epidemiology, Novogireevskaya Str., 3a, 111123 Moscow, RussiaCentral Research Institute of Epidemiology, Novogireevskaya Str., 3a, 111123 Moscow, RussiaCentral Research Institute of Epidemiology, Novogireevskaya Str., 3a, 111123 Moscow, RussiaCentral Research Institute of Epidemiology, Novogireevskaya Str., 3a, 111123 Moscow, RussiaCentral Research Institute of Epidemiology, Novogireevskaya Str., 3a, 111123 Moscow, Russia<i>Salmonella enterica</i> is an important foodborne pathogen worldwide. Ciprofloxacin and extended-spectrum cephalosporins are the common first-line antimicrobial drugs for the treatment of salmonellosis, antimicrobial resistance genes for which are mostly transferred via plasmids. The goal of this work was to perform genomic analysis of plasmids from foodborne <i>S. enterica</i> isolates obtained in Russia based on whole-genome sequencing. In the current study, 11 multidrug-resistant samples isolated in 2021 from 8 regions of Russia were selected based on their resistance to ciprofloxacin and third-generation cephalosporins (CIP-3rd). Whole-genome short-read sequencing (WGS) was performed for all isolates; the samples belonged to five different sequence types (ST32, ST469, ST11, ST142, and ST548) which had different profiles of antimicrobial resistance (AMR) and virulence genes. We have performed additional long-read sequencing of four representative <i>S. enterica</i> isolates, which showed that they carried pESI-like megaplasmids of 202–280 kb length harboring extended-spectrum β-lactamase genes, fluoroquinolone, tetracycline, and aminoglycosides resistance genes, as well as several virulence determinants. We believe that the WGS data obtained will greatly facilitate further studies of foodborne <i>S. enterica</i> isolates epidemiology in terms of their self-transmissible plasmid composition that mediated antimicrobial resistance and virulence determinants conferring selective advantages of this important bacterial pathogen.https://www.mdpi.com/2076-2607/11/2/347<i>Salmonella</i>antibiotic resistancewhole-genome sequencinggenomic epidemiologymobile elementscephalosporins resistance |
| spellingShingle | Anna Egorova Andrey Shelenkov Konstantin Kuleshov Nina Kulikova Aleksey Chernyshkov Igor Manzeniuk Yulia Mikhaylova Vasiliy Akimkin Plasmid Composition, Antimicrobial Resistance and Virulence Genes Profiles of Ciprofloxacin- and Third-Generation Cephalosporin-Resistant Foodborne <i>Salmonella enterica</i> Isolates from Russia Microorganisms <i>Salmonella</i> antibiotic resistance whole-genome sequencing genomic epidemiology mobile elements cephalosporins resistance |
| title | Plasmid Composition, Antimicrobial Resistance and Virulence Genes Profiles of Ciprofloxacin- and Third-Generation Cephalosporin-Resistant Foodborne <i>Salmonella enterica</i> Isolates from Russia |
| title_full | Plasmid Composition, Antimicrobial Resistance and Virulence Genes Profiles of Ciprofloxacin- and Third-Generation Cephalosporin-Resistant Foodborne <i>Salmonella enterica</i> Isolates from Russia |
| title_fullStr | Plasmid Composition, Antimicrobial Resistance and Virulence Genes Profiles of Ciprofloxacin- and Third-Generation Cephalosporin-Resistant Foodborne <i>Salmonella enterica</i> Isolates from Russia |
| title_full_unstemmed | Plasmid Composition, Antimicrobial Resistance and Virulence Genes Profiles of Ciprofloxacin- and Third-Generation Cephalosporin-Resistant Foodborne <i>Salmonella enterica</i> Isolates from Russia |
| title_short | Plasmid Composition, Antimicrobial Resistance and Virulence Genes Profiles of Ciprofloxacin- and Third-Generation Cephalosporin-Resistant Foodborne <i>Salmonella enterica</i> Isolates from Russia |
| title_sort | plasmid composition antimicrobial resistance and virulence genes profiles of ciprofloxacin and third generation cephalosporin resistant foodborne i salmonella enterica i isolates from russia |
| topic | <i>Salmonella</i> antibiotic resistance whole-genome sequencing genomic epidemiology mobile elements cephalosporins resistance |
| url | https://www.mdpi.com/2076-2607/11/2/347 |
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