Citrus huanglongbing: validation of Real-Time PCR (qPCR) for the detection of Candidatus Liberibacter asiaticus and Candidatus Liberibacter americanus in Colombia
Citrus huanglongbing (HLB) is the most destructive citrus disease. Two of the three known HLB-associated Candidatus Liberibacter species were recently found to be present in the Americas. In this study, eggs, nymphs and adults of Diaphorina citri Kuwayama (Hemiptera: Liviidae) and suspect citrus pla...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Centro Editorial of Facultad de Ciencias Agrarias, Universidad Nacional de Colombia
2014-12-01
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| Series: | Agronomía Colombiana |
| Subjects: | |
| Online Access: | http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-99652014000300010&lng=en&tlng=en |
| _version_ | 1818451531276484608 |
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| author | Jorge Evelio Ángel Erick Geovanni Hernández Néstor Andrés Herrera Linda Yhiset Gómez Ángela Patricia Castro Adriana Milena Sepúlveda Everth Emilio Ebratt |
| author_facet | Jorge Evelio Ángel Erick Geovanni Hernández Néstor Andrés Herrera Linda Yhiset Gómez Ángela Patricia Castro Adriana Milena Sepúlveda Everth Emilio Ebratt |
| author_sort | Jorge Evelio Ángel |
| collection | DOAJ |
| description | Citrus huanglongbing (HLB) is the most destructive citrus disease. Two of the three known HLB-associated Candidatus Liberibacter species were recently found to be present in the Americas. In this study, eggs, nymphs and adults of Diaphorina citri Kuwayama (Hemiptera: Liviidae) and suspect citrus plant materials were collected in 25 municipalities in the departments of Cundinamarca, Santander, Valle del Cauca, Meta and Quindio (Colombia). The detection sensitivity, specificity and assay performance of the 16S rDNA-based real-time PCR (qPCR) were validated for the field survey of the disease in Colombia. The validation confirmed the reliability and robustness of the real-time PCR method for the detection of HLB bacteria in host citrus plant tissues and the vector D. citri. The diagnosis was performed for Candidatus Liberibacter asiaticus (Ca. L. asiaticus) and for Candidatus Liberibacter americanus (Ca. L. americanus) on 168 citrus plant material samples and 239 insect samples. Neither Ca. L. asiaticus nor Ca. L. americanus were detected in the host plants or insects vector, confirming the absence of the disease in the citrus-producing areas of Colombia. |
| first_indexed | 2024-12-14T21:08:40Z |
| format | Article |
| id | doaj.art-ba38aeb1ad7a4e1ba1ac9be59c54ca26 |
| institution | Directory Open Access Journal |
| issn | 0120-9965 |
| language | English |
| last_indexed | 2024-12-14T21:08:40Z |
| publishDate | 2014-12-01 |
| publisher | Centro Editorial of Facultad de Ciencias Agrarias, Universidad Nacional de Colombia |
| record_format | Article |
| series | Agronomía Colombiana |
| spelling | doaj.art-ba38aeb1ad7a4e1ba1ac9be59c54ca262022-12-21T22:47:20ZengCentro Editorial of Facultad de Ciencias Agrarias, Universidad Nacional de ColombiaAgronomía Colombiana0120-99652014-12-0132337738910.15446/agron.colomb.v32n3.44069S0120-99652014000300010Citrus huanglongbing: validation of Real-Time PCR (qPCR) for the detection of Candidatus Liberibacter asiaticus and Candidatus Liberibacter americanus in ColombiaJorge Evelio Ángel0Erick Geovanni Hernández1Néstor Andrés Herrera2Linda Yhiset Gómez3Ángela Patricia Castro4Adriana Milena Sepúlveda5Everth Emilio Ebratt6Instituto Colombiano Agropecuario (ICA)Instituto Colombiano Agropecuario (ICA)Instituto Colombiano Agropecuario (ICA)Instituto Colombiano Agropecuario (ICA)Instituto Colombiano Agropecuario (ICA)Instituto Colombiano Agropecuario (ICA)Instituto Colombiano Agropecuario (ICA)Citrus huanglongbing (HLB) is the most destructive citrus disease. Two of the three known HLB-associated Candidatus Liberibacter species were recently found to be present in the Americas. In this study, eggs, nymphs and adults of Diaphorina citri Kuwayama (Hemiptera: Liviidae) and suspect citrus plant materials were collected in 25 municipalities in the departments of Cundinamarca, Santander, Valle del Cauca, Meta and Quindio (Colombia). The detection sensitivity, specificity and assay performance of the 16S rDNA-based real-time PCR (qPCR) were validated for the field survey of the disease in Colombia. The validation confirmed the reliability and robustness of the real-time PCR method for the detection of HLB bacteria in host citrus plant tissues and the vector D. citri. The diagnosis was performed for Candidatus Liberibacter asiaticus (Ca. L. asiaticus) and for Candidatus Liberibacter americanus (Ca. L. americanus) on 168 citrus plant material samples and 239 insect samples. Neither Ca. L. asiaticus nor Ca. L. americanus were detected in the host plants or insects vector, confirming the absence of the disease in the citrus-producing areas of Colombia.http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-99652014000300010&lng=en&tlng=enDiaphoroina citriHLBPsyllidaeqPCR16S rDNA |
| spellingShingle | Jorge Evelio Ángel Erick Geovanni Hernández Néstor Andrés Herrera Linda Yhiset Gómez Ángela Patricia Castro Adriana Milena Sepúlveda Everth Emilio Ebratt Citrus huanglongbing: validation of Real-Time PCR (qPCR) for the detection of Candidatus Liberibacter asiaticus and Candidatus Liberibacter americanus in Colombia Agronomía Colombiana Diaphoroina citri HLB Psyllidae qPCR 16S rDNA |
| title | Citrus huanglongbing: validation of Real-Time PCR (qPCR) for the detection of Candidatus Liberibacter asiaticus and Candidatus Liberibacter americanus in Colombia |
| title_full | Citrus huanglongbing: validation of Real-Time PCR (qPCR) for the detection of Candidatus Liberibacter asiaticus and Candidatus Liberibacter americanus in Colombia |
| title_fullStr | Citrus huanglongbing: validation of Real-Time PCR (qPCR) for the detection of Candidatus Liberibacter asiaticus and Candidatus Liberibacter americanus in Colombia |
| title_full_unstemmed | Citrus huanglongbing: validation of Real-Time PCR (qPCR) for the detection of Candidatus Liberibacter asiaticus and Candidatus Liberibacter americanus in Colombia |
| title_short | Citrus huanglongbing: validation of Real-Time PCR (qPCR) for the detection of Candidatus Liberibacter asiaticus and Candidatus Liberibacter americanus in Colombia |
| title_sort | citrus huanglongbing validation of real time pcr qpcr for the detection of candidatus liberibacter asiaticus and candidatus liberibacter americanus in colombia |
| topic | Diaphoroina citri HLB Psyllidae qPCR 16S rDNA |
| url | http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-99652014000300010&lng=en&tlng=en |
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