Embryo aggregation regulates in vitro stress conditions to promote developmental competence in pigs
Embryo aggregation is a useful method to produce blastocysts with high developmental competence to generate more offspring in various mammals, but the underlying mechanism(s) regarding the beneficial effects are largely unknown. In this study, we investigated the effects of embryo aggregation using...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
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PeerJ Inc.
2019-12-01
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| Online Access: | https://peerj.com/articles/8143.pdf |
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| author | Pil-Soo Jeong Seung-Bin Yoon Mun-Hyeong Lee Hee-Chang Son Hwal-Yong Lee Sanghoon Lee Bon-Sang Koo Kang-Jin Jeong Jong-Hee Lee Yeung Bae Jin Bong-Seok Song Ji-Su Kim Sun-Uk Kim Deog-Bon Koo Bo-Woong Sim |
| author_facet | Pil-Soo Jeong Seung-Bin Yoon Mun-Hyeong Lee Hee-Chang Son Hwal-Yong Lee Sanghoon Lee Bon-Sang Koo Kang-Jin Jeong Jong-Hee Lee Yeung Bae Jin Bong-Seok Song Ji-Su Kim Sun-Uk Kim Deog-Bon Koo Bo-Woong Sim |
| author_sort | Pil-Soo Jeong |
| collection | DOAJ |
| description | Embryo aggregation is a useful method to produce blastocysts with high developmental competence to generate more offspring in various mammals, but the underlying mechanism(s) regarding the beneficial effects are largely unknown. In this study, we investigated the effects of embryo aggregation using 4-cell stage embryos in in vitro developmental competence and the relationship of stress conditions in porcine early embryogenesis. We conducted aggregation using the well of the well system and confirmed that aggregation using two or three embryos was useful for obtaining blastocysts. Aggregated embryos significantly improved developmental competence, including blastocyst formation rate, blastomere number, ICM/TE ratio, and cellular survival rate, compared to non-aggregated embryos. Investigation into the relationship between embryo aggregation and stress conditions revealed that mitochondrial function increased, and oxidative and endoplasmic reticulum (ER)-stress decreased compared to 1X (non-aggregated embryos) blastocysts. In addition, 3X (three-embryo aggregated) blastocysts increased the expression of pluripotency, anti-apoptosis, and implantation related genes, and decreased expression of pro-apoptosis related genes. Therefore, these findings indicate that embryo aggregation regulates in vitro stress conditions to increase developmental competence and contributes to the in vitro production of high-quality embryos and the large-scale production of transgenic and chimeric pigs. |
| first_indexed | 2024-03-09T06:32:20Z |
| format | Article |
| id | doaj.art-ba399605cc2e4cd3aeb48fdb2d300b84 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:32:20Z |
| publishDate | 2019-12-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj.art-ba399605cc2e4cd3aeb48fdb2d300b842023-12-03T11:04:35ZengPeerJ Inc.PeerJ2167-83592019-12-017e814310.7717/peerj.8143Embryo aggregation regulates in vitro stress conditions to promote developmental competence in pigsPil-Soo Jeong0Seung-Bin Yoon1Mun-Hyeong Lee2Hee-Chang Son3Hwal-Yong Lee4Sanghoon Lee5Bon-Sang Koo6Kang-Jin Jeong7Jong-Hee Lee8Yeung Bae Jin9Bong-Seok Song10Ji-Su Kim11Sun-Uk Kim12Deog-Bon Koo13Bo-Woong Sim14Futuristic Animal Resource & Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongju, Republic of KoreaFuturistic Animal Resource & Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongju, Republic of KoreaFuturistic Animal Resource & Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongju, Republic of KoreaFuturistic Animal Resource & Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongju, Republic of KoreaNational Primate Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongju, Republic of KoreaFuturistic Animal Resource & Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongju, Republic of KoreaNational Primate Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongju, Republic of KoreaNational Primate Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongju, Republic of KoreaNational Primate Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongju, Republic of KoreaNational Primate Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongju, Republic of KoreaFuturistic Animal Resource & Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongju, Republic of KoreaFuturistic Animal Resource & Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongju, Republic of KoreaFuturistic Animal Resource & Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongju, Republic of KoreaDepartment of Biotechnology, College of Engineering, Daegu University, Gyeongsan, Republic of KoreaFuturistic Animal Resource & Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongju, Republic of KoreaEmbryo aggregation is a useful method to produce blastocysts with high developmental competence to generate more offspring in various mammals, but the underlying mechanism(s) regarding the beneficial effects are largely unknown. In this study, we investigated the effects of embryo aggregation using 4-cell stage embryos in in vitro developmental competence and the relationship of stress conditions in porcine early embryogenesis. We conducted aggregation using the well of the well system and confirmed that aggregation using two or three embryos was useful for obtaining blastocysts. Aggregated embryos significantly improved developmental competence, including blastocyst formation rate, blastomere number, ICM/TE ratio, and cellular survival rate, compared to non-aggregated embryos. Investigation into the relationship between embryo aggregation and stress conditions revealed that mitochondrial function increased, and oxidative and endoplasmic reticulum (ER)-stress decreased compared to 1X (non-aggregated embryos) blastocysts. In addition, 3X (three-embryo aggregated) blastocysts increased the expression of pluripotency, anti-apoptosis, and implantation related genes, and decreased expression of pro-apoptosis related genes. Therefore, these findings indicate that embryo aggregation regulates in vitro stress conditions to increase developmental competence and contributes to the in vitro production of high-quality embryos and the large-scale production of transgenic and chimeric pigs.https://peerj.com/articles/8143.pdfPig embryo aggregationBlastocyst qualityMitochondrial functionReactive oxygen speciesEndoplasmic reticulum stress |
| spellingShingle | Pil-Soo Jeong Seung-Bin Yoon Mun-Hyeong Lee Hee-Chang Son Hwal-Yong Lee Sanghoon Lee Bon-Sang Koo Kang-Jin Jeong Jong-Hee Lee Yeung Bae Jin Bong-Seok Song Ji-Su Kim Sun-Uk Kim Deog-Bon Koo Bo-Woong Sim Embryo aggregation regulates in vitro stress conditions to promote developmental competence in pigs PeerJ Pig embryo aggregation Blastocyst quality Mitochondrial function Reactive oxygen species Endoplasmic reticulum stress |
| title | Embryo aggregation regulates in vitro stress conditions to promote developmental competence in pigs |
| title_full | Embryo aggregation regulates in vitro stress conditions to promote developmental competence in pigs |
| title_fullStr | Embryo aggregation regulates in vitro stress conditions to promote developmental competence in pigs |
| title_full_unstemmed | Embryo aggregation regulates in vitro stress conditions to promote developmental competence in pigs |
| title_short | Embryo aggregation regulates in vitro stress conditions to promote developmental competence in pigs |
| title_sort | embryo aggregation regulates in vitro stress conditions to promote developmental competence in pigs |
| topic | Pig embryo aggregation Blastocyst quality Mitochondrial function Reactive oxygen species Endoplasmic reticulum stress |
| url | https://peerj.com/articles/8143.pdf |
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