Spread of Linezolid-Resistant <i>Enterococcus</i> spp. in Human Clinical Isolates in the Czech Republic
The aim of this study was to map and investigate linezolid resistance mechanisms in linezolid-resistant enterococci in the Czech Republic from 2009 to 2019. Altogether, 1442 isolates of <i>Enterococcus faecium</i> and <i>Enterococcus faecalis</i> were examined in the National...
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MDPI AG
2021-02-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/10/2/219 |
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| author | Lucia Mališová Vladislav Jakubů Katarína Pomorská Martin Musílek Helena Žemličková |
| author_facet | Lucia Mališová Vladislav Jakubů Katarína Pomorská Martin Musílek Helena Žemličková |
| author_sort | Lucia Mališová |
| collection | DOAJ |
| description | The aim of this study was to map and investigate linezolid resistance mechanisms in linezolid-resistant enterococci in the Czech Republic from 2009 to 2019. Altogether, 1442 isolates of <i>Enterococcus faecium</i> and <i>Enterococcus faecalis</i> were examined in the National Reference Laboratory for Antibiotics. Among them, 8% of isolates (<i>n</i> = 115) were resistant to linezolid (<i>E. faecium</i>/<i>n</i> = 106, <i>E. faecalis</i>/<i>n</i> = 9). Only three strains of <i>E. faecium</i> were resistant to tigecycline, 72.6% of isolates were resistant to vancomycin. One isolate of <i>E. faecium</i> harbored the <i>cfr</i> gene. The majority (87%, <i>n</i> = 11) of <i>E. faecium</i> strains were resistant to linezolid because of the mutation G2576T in the domain V of the 23S rRNA. This mutation was detected also in two strains of <i>E. faecalis</i>. The presence of the <i>optrA</i> gene was the dominant mechanism of linezolid resistance in <i>E. faecalis</i> isolates. None of enterococci contained <i>cfrB</i>, <i>poxtA</i> genes, or any amino acid mutation in genes encoding ribosomal proteins. No mechanism of resistance was identified in 4 out of 106 <i>E. faecium</i> linezolid resistant isolates in this study. Seventeen sequence types (STs) including four novel STs were identified in this work. Clonal complex CC17 was found in all <i>E. faecium</i> isolates. |
| first_indexed | 2024-03-09T00:38:47Z |
| format | Article |
| id | doaj.art-ba39a936302a44ea92f66c8e9fa09d13 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-09T00:38:47Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-ba39a936302a44ea92f66c8e9fa09d132023-12-11T17:59:08ZengMDPI AGAntibiotics2079-63822021-02-0110221910.3390/antibiotics10020219Spread of Linezolid-Resistant <i>Enterococcus</i> spp. in Human Clinical Isolates in the Czech RepublicLucia Mališová0Vladislav Jakubů1Katarína Pomorská2Martin Musílek3Helena Žemličková4National Reference Laboratory for Antibiotics, Centre for Epidemiology and Microbiology, National Institute of Public Health, 10000 Prague, Czech RepublicNational Reference Laboratory for Antibiotics, Centre for Epidemiology and Microbiology, National Institute of Public Health, 10000 Prague, Czech RepublicNational Reference Laboratory for Antibiotics, Centre for Epidemiology and Microbiology, National Institute of Public Health, 10000 Prague, Czech RepublicNational Reference Laboratory for Meningococcal Infections, Centre for Epidemiology and Microbiology, National Institute of Public Health, 10000 Prague, Czech RepublicNational Reference Laboratory for Antibiotics, Centre for Epidemiology and Microbiology, National Institute of Public Health, 10000 Prague, Czech RepublicThe aim of this study was to map and investigate linezolid resistance mechanisms in linezolid-resistant enterococci in the Czech Republic from 2009 to 2019. Altogether, 1442 isolates of <i>Enterococcus faecium</i> and <i>Enterococcus faecalis</i> were examined in the National Reference Laboratory for Antibiotics. Among them, 8% of isolates (<i>n</i> = 115) were resistant to linezolid (<i>E. faecium</i>/<i>n</i> = 106, <i>E. faecalis</i>/<i>n</i> = 9). Only three strains of <i>E. faecium</i> were resistant to tigecycline, 72.6% of isolates were resistant to vancomycin. One isolate of <i>E. faecium</i> harbored the <i>cfr</i> gene. The majority (87%, <i>n</i> = 11) of <i>E. faecium</i> strains were resistant to linezolid because of the mutation G2576T in the domain V of the 23S rRNA. This mutation was detected also in two strains of <i>E. faecalis</i>. The presence of the <i>optrA</i> gene was the dominant mechanism of linezolid resistance in <i>E. faecalis</i> isolates. None of enterococci contained <i>cfrB</i>, <i>poxtA</i> genes, or any amino acid mutation in genes encoding ribosomal proteins. No mechanism of resistance was identified in 4 out of 106 <i>E. faecium</i> linezolid resistant isolates in this study. Seventeen sequence types (STs) including four novel STs were identified in this work. Clonal complex CC17 was found in all <i>E. faecium</i> isolates.https://www.mdpi.com/2079-6382/10/2/219<i>Enterococcus faecium</i><i>Enterococcus faecalis</i>linezolid resistance23S rRNAoptrA |
| spellingShingle | Lucia Mališová Vladislav Jakubů Katarína Pomorská Martin Musílek Helena Žemličková Spread of Linezolid-Resistant <i>Enterococcus</i> spp. in Human Clinical Isolates in the Czech Republic Antibiotics <i>Enterococcus faecium</i> <i>Enterococcus faecalis</i> linezolid resistance 23S rRNA optrA |
| title | Spread of Linezolid-Resistant <i>Enterococcus</i> spp. in Human Clinical Isolates in the Czech Republic |
| title_full | Spread of Linezolid-Resistant <i>Enterococcus</i> spp. in Human Clinical Isolates in the Czech Republic |
| title_fullStr | Spread of Linezolid-Resistant <i>Enterococcus</i> spp. in Human Clinical Isolates in the Czech Republic |
| title_full_unstemmed | Spread of Linezolid-Resistant <i>Enterococcus</i> spp. in Human Clinical Isolates in the Czech Republic |
| title_short | Spread of Linezolid-Resistant <i>Enterococcus</i> spp. in Human Clinical Isolates in the Czech Republic |
| title_sort | spread of linezolid resistant i enterococcus i spp in human clinical isolates in the czech republic |
| topic | <i>Enterococcus faecium</i> <i>Enterococcus faecalis</i> linezolid resistance 23S rRNA optrA |
| url | https://www.mdpi.com/2079-6382/10/2/219 |
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