Synergistic Effects of Some Methoxyflavones Extracted from Rhizome of <i>Kaempferia parviflora</i> Combined with Gentamicin against Carbapenem-Resistant Strains of <i>Klebsiella pneumoniae</i>, <i>Pseudomonas aeruginosa</i>, and <i>Acinetobacter baumannii</i>
The present study aimed to investigate the antibacterial activity of ethanolic <i>Kaempferia parviflora</i> extracts and the combined effects of the plant’s specific compounds with gentamicin against clinical strains of carbapenem-resistant <i>Klebsiella pneumoniae</i>, <i...
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MDPI AG
2022-11-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/11/22/3128 |
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| author | Siriwoot Sookkhee Choompone Sakonwasun Pitchaya Mungkornasawakul Phadungkiat Khamnoi Nitwara Wikan Wutigri Nimlamool |
| author_facet | Siriwoot Sookkhee Choompone Sakonwasun Pitchaya Mungkornasawakul Phadungkiat Khamnoi Nitwara Wikan Wutigri Nimlamool |
| author_sort | Siriwoot Sookkhee |
| collection | DOAJ |
| description | The present study aimed to investigate the antibacterial activity of ethanolic <i>Kaempferia parviflora</i> extracts and the combined effects of the plant’s specific compounds with gentamicin against clinical strains of carbapenem-resistant <i>Klebsiella pneumoniae</i>, <i>Pseudomonas aeruginosa</i>, and <i>Acinetobacter baumannii</i>. The minimal inhibitory concentrations (MIC) of gentamicin and <i>Kaempferia parviflora</i> extracts against the tested bacterial strains were determined by using broth microdilution. The combined effects of <i>Kaempferia parviflora</i> extract and gentamicin were investigated by using a checkerboard assay and expressed as a fractional inhibitory concentration index (FICI). Crude ethanolic extract of <i>Kaempferia parviflora</i> showed the lowest median values of MIC towards the tested isolates (<i>n</i> = 10) of these tested bacteria at doses of 64 µg/mL, compared to those of other <i>Kaempferia extracts</i>. Among the isolated compounds, only three compounds, namely 3,5,7-trimethoxyflavone, 3,5,7,3′4′-pentamethoxyflavone, and 5,7,4′-trimethoxyflavone, were identified by NMR structural analysis. According to their FICIs, the synergistic effects of gentamicin combined with 3,5,7,3′4′-pentamethoxyflavone were approximately 90%, 90%, and 80% of tested carbapenem-resistant <i>Klebsiella pneumoniae</i> (CRKP), <i>Pseudomonas aeruginosa</i> (CRPA), and <i>Acinetobacter baumannii</i> (CRAB), respectively. The present study concluded that 3,5,7,3′4′-pentamethoxyflavone extracted from <i>Kaempferia parviflora</i> potentiated the antibacterial action of gentamicin to combat bacterial resistance against the tested bacteria. |
| first_indexed | 2024-03-09T18:04:16Z |
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| id | doaj.art-ba3baa74d698462ea0d7b61abf0d9ff5 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T18:04:16Z |
| publishDate | 2022-11-01 |
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| series | Plants |
| spelling | doaj.art-ba3baa74d698462ea0d7b61abf0d9ff52023-11-24T09:40:04ZengMDPI AGPlants2223-77472022-11-011122312810.3390/plants11223128Synergistic Effects of Some Methoxyflavones Extracted from Rhizome of <i>Kaempferia parviflora</i> Combined with Gentamicin against Carbapenem-Resistant Strains of <i>Klebsiella pneumoniae</i>, <i>Pseudomonas aeruginosa</i>, and <i>Acinetobacter baumannii</i>Siriwoot Sookkhee0Choompone Sakonwasun1Pitchaya Mungkornasawakul2Phadungkiat Khamnoi3Nitwara Wikan4Wutigri Nimlamool5Department of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, ThailandDiagnostic Laboratory Unit, Maharaj Nakorn Chiang Mai Hospital, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Pharmacology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Pharmacology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, ThailandThe present study aimed to investigate the antibacterial activity of ethanolic <i>Kaempferia parviflora</i> extracts and the combined effects of the plant’s specific compounds with gentamicin against clinical strains of carbapenem-resistant <i>Klebsiella pneumoniae</i>, <i>Pseudomonas aeruginosa</i>, and <i>Acinetobacter baumannii</i>. The minimal inhibitory concentrations (MIC) of gentamicin and <i>Kaempferia parviflora</i> extracts against the tested bacterial strains were determined by using broth microdilution. The combined effects of <i>Kaempferia parviflora</i> extract and gentamicin were investigated by using a checkerboard assay and expressed as a fractional inhibitory concentration index (FICI). Crude ethanolic extract of <i>Kaempferia parviflora</i> showed the lowest median values of MIC towards the tested isolates (<i>n</i> = 10) of these tested bacteria at doses of 64 µg/mL, compared to those of other <i>Kaempferia extracts</i>. Among the isolated compounds, only three compounds, namely 3,5,7-trimethoxyflavone, 3,5,7,3′4′-pentamethoxyflavone, and 5,7,4′-trimethoxyflavone, were identified by NMR structural analysis. According to their FICIs, the synergistic effects of gentamicin combined with 3,5,7,3′4′-pentamethoxyflavone were approximately 90%, 90%, and 80% of tested carbapenem-resistant <i>Klebsiella pneumoniae</i> (CRKP), <i>Pseudomonas aeruginosa</i> (CRPA), and <i>Acinetobacter baumannii</i> (CRAB), respectively. The present study concluded that 3,5,7,3′4′-pentamethoxyflavone extracted from <i>Kaempferia parviflora</i> potentiated the antibacterial action of gentamicin to combat bacterial resistance against the tested bacteria.https://www.mdpi.com/2223-7747/11/22/3128synergismantibacterial effectmethoxyflavones<i>Kaempferia parviflora</i>carbapenem resistance |
| spellingShingle | Siriwoot Sookkhee Choompone Sakonwasun Pitchaya Mungkornasawakul Phadungkiat Khamnoi Nitwara Wikan Wutigri Nimlamool Synergistic Effects of Some Methoxyflavones Extracted from Rhizome of <i>Kaempferia parviflora</i> Combined with Gentamicin against Carbapenem-Resistant Strains of <i>Klebsiella pneumoniae</i>, <i>Pseudomonas aeruginosa</i>, and <i>Acinetobacter baumannii</i> Plants synergism antibacterial effect methoxyflavones <i>Kaempferia parviflora</i> carbapenem resistance |
| title | Synergistic Effects of Some Methoxyflavones Extracted from Rhizome of <i>Kaempferia parviflora</i> Combined with Gentamicin against Carbapenem-Resistant Strains of <i>Klebsiella pneumoniae</i>, <i>Pseudomonas aeruginosa</i>, and <i>Acinetobacter baumannii</i> |
| title_full | Synergistic Effects of Some Methoxyflavones Extracted from Rhizome of <i>Kaempferia parviflora</i> Combined with Gentamicin against Carbapenem-Resistant Strains of <i>Klebsiella pneumoniae</i>, <i>Pseudomonas aeruginosa</i>, and <i>Acinetobacter baumannii</i> |
| title_fullStr | Synergistic Effects of Some Methoxyflavones Extracted from Rhizome of <i>Kaempferia parviflora</i> Combined with Gentamicin against Carbapenem-Resistant Strains of <i>Klebsiella pneumoniae</i>, <i>Pseudomonas aeruginosa</i>, and <i>Acinetobacter baumannii</i> |
| title_full_unstemmed | Synergistic Effects of Some Methoxyflavones Extracted from Rhizome of <i>Kaempferia parviflora</i> Combined with Gentamicin against Carbapenem-Resistant Strains of <i>Klebsiella pneumoniae</i>, <i>Pseudomonas aeruginosa</i>, and <i>Acinetobacter baumannii</i> |
| title_short | Synergistic Effects of Some Methoxyflavones Extracted from Rhizome of <i>Kaempferia parviflora</i> Combined with Gentamicin against Carbapenem-Resistant Strains of <i>Klebsiella pneumoniae</i>, <i>Pseudomonas aeruginosa</i>, and <i>Acinetobacter baumannii</i> |
| title_sort | synergistic effects of some methoxyflavones extracted from rhizome of i kaempferia parviflora i combined with gentamicin against carbapenem resistant strains of i klebsiella pneumoniae i i pseudomonas aeruginosa i and i acinetobacter baumannii i |
| topic | synergism antibacterial effect methoxyflavones <i>Kaempferia parviflora</i> carbapenem resistance |
| url | https://www.mdpi.com/2223-7747/11/22/3128 |
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