Blending DNA binding dyes to improve detection in real-time PCR

Blending DNA binding dyes to improve detection in real-time PCR

The success of real-time PCR (qPCR) analysis is partly limited by the presence of inhibitory compounds in the nucleic acid samples. For example, humic acid (HA) from soil and aqueous sediment interferes with amplification and also quenches the fluorescence of double-stranded (ds) DNA binding dyes, t...

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Bibliographic Details
Main Authors:	Linda Jansson, Marianne Koliana, Maja Sidstedt, Johannes Hedman
Format:	Article
Language:	English
Published:	Elsevier 2017-03-01
Series:	Biotechnology Reports
Subjects:	Fluorescence quenching Humic acid PCR inhibition qPCR Soil
Online Access:	http://www.sciencedirect.com/science/article/pii/S2215017X17300152

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