Simultaneous Determination of Original, Degraded Ginsenosides and Aglycones by Ultra High Performance Liquid Chromatography Coupled with Quadrupole Time-of-Flight Mass Spectrometry for Quantitative Evaluation of Du-Shen-Tang, the Decoction of Ginseng
In the present study, an ultra-high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-QTOF-MS) method for simultaneous determination of eleven original, fourteen degraded ginsenosides and five aglycones was developed and validated to quantitatively eva...
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| Format: | Article |
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MDPI AG
2014-04-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/19/4/4083 |
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| author | Shan-Shan Zhou Jin-Di Xu He Zhu Hong Shen Jun Xu Qian Mao Song-Lin Li Ru Yan |
| author_facet | Shan-Shan Zhou Jin-Di Xu He Zhu Hong Shen Jun Xu Qian Mao Song-Lin Li Ru Yan |
| author_sort | Shan-Shan Zhou |
| collection | DOAJ |
| description | In the present study, an ultra-high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-QTOF-MS) method for simultaneous determination of eleven original, fourteen degraded ginsenosides and five aglycones was developed and validated to quantitatively evaluate the transformation of ginsenosides during preparation of Du-Shen-Tang, the decoction of ginseng. Both positive and negative modes as well as the step wave ion transfer optics technology were used to increase the detection sensitivity of QTOF-MS. The extracting ion mode based on the quasi-molecular ions, molecular ions and fragment ions characteristic to each analyte was used to increase the selectivity for quantitative analysis. Under the optimized UHPLC and QTOF-MS conditions, the 30 analytes with different polarities were separated (except for Re and Rg1) within 26 min. The developed method was applied for the quantitative comparison of Du-Shen-Tang and its raw materials derived from Asian ginseng (ASG) and American ginseng (AMG), respectively. It was found that the contents of the original ginsenosides decreased from 26,053.09 to 19,393.29 μg/g or 45,027.72 to 41,865.39 μg/g, whereas the degraded ginsenosides and aglycones increased from 159.72 to 685.37 μg/g or 676.54 to 1,502.26 μg/g in Du-Shen-Tang samples of ASG or AMG when compared with their raw materials, indicating that decocting could dramatically increase the proportion of the less polar degraded ginsenosides in Du-Shen-Tang. Whether these changed proportions of different polar ginsenosides could affect the bioactivities of the decoctions and their raw materials derived from ASG and AMG deserves further investigation. |
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| spelling | doaj.art-ba3c1613f34e4beeba6d4da37ef3360f2022-12-22T02:56:29ZengMDPI AGMolecules1420-30492014-04-011944083410410.3390/molecules19044083molecules19044083Simultaneous Determination of Original, Degraded Ginsenosides and Aglycones by Ultra High Performance Liquid Chromatography Coupled with Quadrupole Time-of-Flight Mass Spectrometry for Quantitative Evaluation of Du-Shen-Tang, the Decoction of GinsengShan-Shan Zhou0Jin-Di Xu1He Zhu2Hong Shen3Jun Xu4Qian Mao5Song-Lin Li6Ru Yan7Department of Pharmaceutical Analysis and Metabolomics, Jiangsu Province Academy of Traditional Chinese Medicine and Jiangsu Branch of China Academy of Chinese Medical Sciences, Nanjing 210028, ChinaDepartment of Pharmaceutical Analysis and Metabolomics, Jiangsu Province Academy of Traditional Chinese Medicine and Jiangsu Branch of China Academy of Chinese Medical Sciences, Nanjing 210028, ChinaDepartment of Pharmaceutical Analysis and Metabolomics, Jiangsu Province Academy of Traditional Chinese Medicine and Jiangsu Branch of China Academy of Chinese Medical Sciences, Nanjing 210028, ChinaDepartment of Pharmaceutical Analysis and Metabolomics, Jiangsu Province Academy of Traditional Chinese Medicine and Jiangsu Branch of China Academy of Chinese Medical Sciences, Nanjing 210028, ChinaDepartment of Pharmaceutical Analysis and Metabolomics, Jiangsu Province Academy of Traditional Chinese Medicine and Jiangsu Branch of China Academy of Chinese Medical Sciences, Nanjing 210028, ChinaDepartment of Pharmaceutical Analysis and Metabolomics, Jiangsu Province Academy of Traditional Chinese Medicine and Jiangsu Branch of China Academy of Chinese Medical Sciences, Nanjing 210028, ChinaDepartment of Pharmaceutical Analysis and Metabolomics, Jiangsu Province Academy of Traditional Chinese Medicine and Jiangsu Branch of China Academy of Chinese Medical Sciences, Nanjing 210028, ChinaState Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, ChinaIn the present study, an ultra-high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-QTOF-MS) method for simultaneous determination of eleven original, fourteen degraded ginsenosides and five aglycones was developed and validated to quantitatively evaluate the transformation of ginsenosides during preparation of Du-Shen-Tang, the decoction of ginseng. Both positive and negative modes as well as the step wave ion transfer optics technology were used to increase the detection sensitivity of QTOF-MS. The extracting ion mode based on the quasi-molecular ions, molecular ions and fragment ions characteristic to each analyte was used to increase the selectivity for quantitative analysis. Under the optimized UHPLC and QTOF-MS conditions, the 30 analytes with different polarities were separated (except for Re and Rg1) within 26 min. The developed method was applied for the quantitative comparison of Du-Shen-Tang and its raw materials derived from Asian ginseng (ASG) and American ginseng (AMG), respectively. It was found that the contents of the original ginsenosides decreased from 26,053.09 to 19,393.29 μg/g or 45,027.72 to 41,865.39 μg/g, whereas the degraded ginsenosides and aglycones increased from 159.72 to 685.37 μg/g or 676.54 to 1,502.26 μg/g in Du-Shen-Tang samples of ASG or AMG when compared with their raw materials, indicating that decocting could dramatically increase the proportion of the less polar degraded ginsenosides in Du-Shen-Tang. Whether these changed proportions of different polar ginsenosides could affect the bioactivities of the decoctions and their raw materials derived from ASG and AMG deserves further investigation.http://www.mdpi.com/1420-3049/19/4/4083UHPLC-QTOF-MSginsengdegraded ginsenosidesquantificationDu-Shen-Tangdecoction |
| spellingShingle | Shan-Shan Zhou Jin-Di Xu He Zhu Hong Shen Jun Xu Qian Mao Song-Lin Li Ru Yan Simultaneous Determination of Original, Degraded Ginsenosides and Aglycones by Ultra High Performance Liquid Chromatography Coupled with Quadrupole Time-of-Flight Mass Spectrometry for Quantitative Evaluation of Du-Shen-Tang, the Decoction of Ginseng Molecules UHPLC-QTOF-MS ginseng degraded ginsenosides quantification Du-Shen-Tang decoction |
| title | Simultaneous Determination of Original, Degraded Ginsenosides and Aglycones by Ultra High Performance Liquid Chromatography Coupled with Quadrupole Time-of-Flight Mass Spectrometry for Quantitative Evaluation of Du-Shen-Tang, the Decoction of Ginseng |
| title_full | Simultaneous Determination of Original, Degraded Ginsenosides and Aglycones by Ultra High Performance Liquid Chromatography Coupled with Quadrupole Time-of-Flight Mass Spectrometry for Quantitative Evaluation of Du-Shen-Tang, the Decoction of Ginseng |
| title_fullStr | Simultaneous Determination of Original, Degraded Ginsenosides and Aglycones by Ultra High Performance Liquid Chromatography Coupled with Quadrupole Time-of-Flight Mass Spectrometry for Quantitative Evaluation of Du-Shen-Tang, the Decoction of Ginseng |
| title_full_unstemmed | Simultaneous Determination of Original, Degraded Ginsenosides and Aglycones by Ultra High Performance Liquid Chromatography Coupled with Quadrupole Time-of-Flight Mass Spectrometry for Quantitative Evaluation of Du-Shen-Tang, the Decoction of Ginseng |
| title_short | Simultaneous Determination of Original, Degraded Ginsenosides and Aglycones by Ultra High Performance Liquid Chromatography Coupled with Quadrupole Time-of-Flight Mass Spectrometry for Quantitative Evaluation of Du-Shen-Tang, the Decoction of Ginseng |
| title_sort | simultaneous determination of original degraded ginsenosides and aglycones by ultra high performance liquid chromatography coupled with quadrupole time of flight mass spectrometry for quantitative evaluation of du shen tang the decoction of ginseng |
| topic | UHPLC-QTOF-MS ginseng degraded ginsenosides quantification Du-Shen-Tang decoction |
| url | http://www.mdpi.com/1420-3049/19/4/4083 |
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