Validation of the Investigator 24plex QS Kit: a 6-dye multiplex PCR assay for forensic application in the Chinese Han population
The Investigator 24plex QS Kit (QIAGEN, Hilden, Germany) is a 6-dye fluorescent chemistry short tandem repeat (STR) polymerase chain reaction (PCR) amplification system that simultaneously amplifies 20 of the expanded Combined DNA Index System (CODIS) core STR loci, SE33, DYS391, and the standard se...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Oxford University Press
2019-12-01
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| Series: | Forensic Sciences Research |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/20961790.2019.1665160 |
| _version_ | 1797765290846584832 |
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| author | Ruiyang Tao Chong Chen Xiang Sheng Ruocheng Xia Xiaochun Zhang Jingyi Zhang Zihao Yang Suhua Zhang Chengtao Li |
| author_facet | Ruiyang Tao Chong Chen Xiang Sheng Ruocheng Xia Xiaochun Zhang Jingyi Zhang Zihao Yang Suhua Zhang Chengtao Li |
| author_sort | Ruiyang Tao |
| collection | DOAJ |
| description | The Investigator 24plex QS Kit (QIAGEN, Hilden, Germany) is a 6-dye fluorescent chemistry short tandem repeat (STR) polymerase chain reaction (PCR) amplification system that simultaneously amplifies 20 of the expanded Combined DNA Index System (CODIS) core STR loci, SE33, DYS391, and the standard sex-determining locus, amelogenin, as well as two special internal performance quality sensor controls (QS1 and QS2), which are included in the primer mix to check the PCR performance. This study was designed to be a pilot evaluation of this STR-PCR kit in a Chinese Han population regarding the PCR conditions, sensitivity, precision, accuracy, repeatability, reproducibility, and concordance; tolerance to PCR inhibitors; applicability to real “forensic-type” samples; species specificity; mixture, balance and stutter analyses, and utility in a population investigation. The exhaustive validation studies demonstrated that the Investigator 24plex QS system is accurate, sensitive and robust for STR genotyping. In addition, these genetic markers in the population data in our study indicated that they can also be useful for forensic identification and paternity testing in the Chinese Han population. |
| first_indexed | 2024-03-12T20:08:04Z |
| format | Article |
| id | doaj.art-ba4275fbabc04738826645177ad494b7 |
| institution | Directory Open Access Journal |
| issn | 2096-1790 2471-1411 |
| language | English |
| last_indexed | 2024-03-12T20:08:04Z |
| publishDate | 2019-12-01 |
| publisher | Oxford University Press |
| record_format | Article |
| series | Forensic Sciences Research |
| spelling | doaj.art-ba4275fbabc04738826645177ad494b72023-08-02T01:58:02ZengOxford University PressForensic Sciences Research2096-17902471-14112019-12-01001910.1080/20961790.2019.16651601665160Validation of the Investigator 24plex QS Kit: a 6-dye multiplex PCR assay for forensic application in the Chinese Han populationRuiyang Tao0Chong Chen1Xiang Sheng2Ruocheng Xia3Xiaochun Zhang4Jingyi Zhang5Zihao Yang6Suhua Zhang7Chengtao Li8Sichuan UniversityAcademy of Forensic Sciences, Ministry of JusticeQIAGENAcademy of Forensic Sciences, Ministry of JusticeAcademy of Forensic Sciences, Ministry of JusticeAcademy of Forensic Sciences, Ministry of JusticeAcademy of Forensic Sciences, Ministry of JusticeAcademy of Forensic Sciences, Ministry of JusticeSichuan UniversityThe Investigator 24plex QS Kit (QIAGEN, Hilden, Germany) is a 6-dye fluorescent chemistry short tandem repeat (STR) polymerase chain reaction (PCR) amplification system that simultaneously amplifies 20 of the expanded Combined DNA Index System (CODIS) core STR loci, SE33, DYS391, and the standard sex-determining locus, amelogenin, as well as two special internal performance quality sensor controls (QS1 and QS2), which are included in the primer mix to check the PCR performance. This study was designed to be a pilot evaluation of this STR-PCR kit in a Chinese Han population regarding the PCR conditions, sensitivity, precision, accuracy, repeatability, reproducibility, and concordance; tolerance to PCR inhibitors; applicability to real “forensic-type” samples; species specificity; mixture, balance and stutter analyses, and utility in a population investigation. The exhaustive validation studies demonstrated that the Investigator 24plex QS system is accurate, sensitive and robust for STR genotyping. In addition, these genetic markers in the population data in our study indicated that they can also be useful for forensic identification and paternity testing in the Chinese Han population.http://dx.doi.org/10.1080/20961790.2019.1665160forensic sciencesforensic geneticsshort tandem repeat (str) typinginvestigator 24plex qs kitvalidation study |
| spellingShingle | Ruiyang Tao Chong Chen Xiang Sheng Ruocheng Xia Xiaochun Zhang Jingyi Zhang Zihao Yang Suhua Zhang Chengtao Li Validation of the Investigator 24plex QS Kit: a 6-dye multiplex PCR assay for forensic application in the Chinese Han population Forensic Sciences Research forensic sciences forensic genetics short tandem repeat (str) typing investigator 24plex qs kit validation study |
| title | Validation of the Investigator 24plex QS Kit: a 6-dye multiplex PCR assay for forensic application in the Chinese Han population |
| title_full | Validation of the Investigator 24plex QS Kit: a 6-dye multiplex PCR assay for forensic application in the Chinese Han population |
| title_fullStr | Validation of the Investigator 24plex QS Kit: a 6-dye multiplex PCR assay for forensic application in the Chinese Han population |
| title_full_unstemmed | Validation of the Investigator 24plex QS Kit: a 6-dye multiplex PCR assay for forensic application in the Chinese Han population |
| title_short | Validation of the Investigator 24plex QS Kit: a 6-dye multiplex PCR assay for forensic application in the Chinese Han population |
| title_sort | validation of the investigator 24plex qs kit a 6 dye multiplex pcr assay for forensic application in the chinese han population |
| topic | forensic sciences forensic genetics short tandem repeat (str) typing investigator 24plex qs kit validation study |
| url | http://dx.doi.org/10.1080/20961790.2019.1665160 |
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