Detecção da mutação dupla 86TYR e 1246TYR no gene pfmdr1 em clones de uma amostra de Plasmodium falciparum da África Ocidental, resistente à cloroquina.
Isolates of Plasmodium falciparum from three areas of West Africa were recovered from cryopreservation and their chloroquine-sensitivity were determined in vitro. Of the 90 samples studied, 60 were from Guinea-Bissau (30Resistant/30Sensitive), 15 were from S. Tomé and Príncipe (11Resistant/4Sensitiv...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Ordem dos Médicos
2003-08-01
|
| Series: | Acta Médica Portuguesa |
| Online Access: | https://www.actamedicaportuguesa.com/revista/index.php/amp/article/view/1191 |
| _version_ | 1811242234872332288 |
|---|---|
| author | L Pinheiro S Franco I S Adagu R Rosa V E Rosário D C Warhurst |
| author_facet | L Pinheiro S Franco I S Adagu R Rosa V E Rosário D C Warhurst |
| author_sort | L Pinheiro |
| collection | DOAJ |
| description | Isolates of Plasmodium falciparum from three areas of West Africa were recovered from cryopreservation and their chloroquine-sensitivity were determined in vitro. Of the 90 samples studied, 60 were from Guinea-Bissau (30Resistant/30Sensitive), 15 were from S. Tomé and Príncipe (11Resistant/4Sensitive) and 15 were from Angola (11Resistant/4Sensitive). All the isolates were sensitive to mefloquine. Using the polymerase chain reaction/restriction fragment length polymorphism technique (PCR/RFLP) it was possible to detect two mutations in the pfmdr1 gene, often associated with chloroquine-resistance. 66% of the samples from Guiné-Bissau showed a correlation with chloroquine-resistance while 73% of the samples from São Tomé and Angola altogether had the 86Tyr mutation. The present study on West African isolates and clones showed, for the first time, the presence of a double point mutation in the pfmdr1 gene one being found, up to now, only in South America isolates of Plasmodium falciparum. |
| first_indexed | 2024-04-12T13:47:44Z |
| format | Article |
| id | doaj.art-ba47d0367bd140afb7252bed5afa32fb |
| institution | Directory Open Access Journal |
| issn | 0870-399X 1646-0758 |
| language | English |
| last_indexed | 2024-04-12T13:47:44Z |
| publishDate | 2003-08-01 |
| publisher | Ordem dos Médicos |
| record_format | Article |
| series | Acta Médica Portuguesa |
| spelling | doaj.art-ba47d0367bd140afb7252bed5afa32fb2022-12-22T03:30:37ZengOrdem dos MédicosActa Médica Portuguesa0870-399X1646-07582003-08-0116410.20344/amp.1191Detecção da mutação dupla 86TYR e 1246TYR no gene pfmdr1 em clones de uma amostra de Plasmodium falciparum da África Ocidental, resistente à cloroquina.L Pinheiro0S FrancoI S AdaguR RosaV E RosárioD C WarhurstCentro de Malária e Outras Doenças Tropicais, Instituto de Higiene e Medicina Tropical, Lisboa.Isolates of Plasmodium falciparum from three areas of West Africa were recovered from cryopreservation and their chloroquine-sensitivity were determined in vitro. Of the 90 samples studied, 60 were from Guinea-Bissau (30Resistant/30Sensitive), 15 were from S. Tomé and Príncipe (11Resistant/4Sensitive) and 15 were from Angola (11Resistant/4Sensitive). All the isolates were sensitive to mefloquine. Using the polymerase chain reaction/restriction fragment length polymorphism technique (PCR/RFLP) it was possible to detect two mutations in the pfmdr1 gene, often associated with chloroquine-resistance. 66% of the samples from Guiné-Bissau showed a correlation with chloroquine-resistance while 73% of the samples from São Tomé and Angola altogether had the 86Tyr mutation. The present study on West African isolates and clones showed, for the first time, the presence of a double point mutation in the pfmdr1 gene one being found, up to now, only in South America isolates of Plasmodium falciparum.https://www.actamedicaportuguesa.com/revista/index.php/amp/article/view/1191 |
| spellingShingle | L Pinheiro S Franco I S Adagu R Rosa V E Rosário D C Warhurst Detecção da mutação dupla 86TYR e 1246TYR no gene pfmdr1 em clones de uma amostra de Plasmodium falciparum da África Ocidental, resistente à cloroquina. Acta Médica Portuguesa |
| title | Detecção da mutação dupla 86TYR e 1246TYR no gene pfmdr1 em clones de uma amostra de Plasmodium falciparum da África Ocidental, resistente à cloroquina. |
| title_full | Detecção da mutação dupla 86TYR e 1246TYR no gene pfmdr1 em clones de uma amostra de Plasmodium falciparum da África Ocidental, resistente à cloroquina. |
| title_fullStr | Detecção da mutação dupla 86TYR e 1246TYR no gene pfmdr1 em clones de uma amostra de Plasmodium falciparum da África Ocidental, resistente à cloroquina. |
| title_full_unstemmed | Detecção da mutação dupla 86TYR e 1246TYR no gene pfmdr1 em clones de uma amostra de Plasmodium falciparum da África Ocidental, resistente à cloroquina. |
| title_short | Detecção da mutação dupla 86TYR e 1246TYR no gene pfmdr1 em clones de uma amostra de Plasmodium falciparum da África Ocidental, resistente à cloroquina. |
| title_sort | deteccao da mutacao dupla 86tyr e 1246tyr no gene pfmdr1 em clones de uma amostra de plasmodium falciparum da africa ocidental resistente a cloroquina |
| url | https://www.actamedicaportuguesa.com/revista/index.php/amp/article/view/1191 |
| work_keys_str_mv | AT lpinheiro deteccaodamutacaodupla86tyre1246tyrnogenepfmdr1emclonesdeumaamostradeplasmodiumfalciparumdaafricaocidentalresistenteacloroquina AT sfranco deteccaodamutacaodupla86tyre1246tyrnogenepfmdr1emclonesdeumaamostradeplasmodiumfalciparumdaafricaocidentalresistenteacloroquina AT isadagu deteccaodamutacaodupla86tyre1246tyrnogenepfmdr1emclonesdeumaamostradeplasmodiumfalciparumdaafricaocidentalresistenteacloroquina AT rrosa deteccaodamutacaodupla86tyre1246tyrnogenepfmdr1emclonesdeumaamostradeplasmodiumfalciparumdaafricaocidentalresistenteacloroquina AT verosario deteccaodamutacaodupla86tyre1246tyrnogenepfmdr1emclonesdeumaamostradeplasmodiumfalciparumdaafricaocidentalresistenteacloroquina AT dcwarhurst deteccaodamutacaodupla86tyre1246tyrnogenepfmdr1emclonesdeumaamostradeplasmodiumfalciparumdaafricaocidentalresistenteacloroquina |