Detection of <i>Mannheimia haemolytica</i>-Specific IgG, IgM and IgA in Sera and Their Relationship to Respiratory Disease in Cattle
<i>Mannheimia haemolytica</i> is one of the major causes of bovine respiratory disease in cattle. The organism is the primary bacterium isolated from calves and young cattle affected with enzootic pneumonia. Novel indirect ELISAs were developed and evaluated to enable quantification of a...
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| Format: | Article |
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MDPI AG
2023-05-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/13/9/1531 |
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| author | Korakrit Poonsuk Carita Kordik Matthew Hille Ting-Yu Cheng William B. Crosby Amelia R. Woolums Michael L. Clawson Carol Chitko-McKown Bruce Brodersen John Dustin Loy |
| author_facet | Korakrit Poonsuk Carita Kordik Matthew Hille Ting-Yu Cheng William B. Crosby Amelia R. Woolums Michael L. Clawson Carol Chitko-McKown Bruce Brodersen John Dustin Loy |
| author_sort | Korakrit Poonsuk |
| collection | DOAJ |
| description | <i>Mannheimia haemolytica</i> is one of the major causes of bovine respiratory disease in cattle. The organism is the primary bacterium isolated from calves and young cattle affected with enzootic pneumonia. Novel indirect ELISAs were developed and evaluated to enable quantification of antibody responses to whole cell antigens using <i>M. haemolytica</i> A1 strain P1148. In this study, the ELISAs were initially developed using sera from both <i>M. haemolytica</i>-culture-free and clinically infected cattle, then the final prototypes were tested in the validation phase using a larger set of known-status <i>M. haemolytica</i> sera (<i>n</i> = 145) collected from feedlot cattle. The test showed good inter-assay and intra-assay repeatability. Diagnostic sensitivity and specificity were estimated at 91% and 87% for IgG at a cutoff of S/P ≥ 0.8. IgM diagnostic sensitivity and specificity were 91% and 81% at a cutoff of sample to positive (S/P) ratio ≥ 0.8. IgA diagnostic sensitivity was 89% whereas specificity was 78% at a cutoff of S/P ≥ 0.2. ELISA results of all isotypes were related to the diagnosis of respiratory disease and isolation of <i>M. haemolytica</i> (<i>p</i>-value < 0.05). These data suggest that <i>M. haemolytica</i> ELISAs can be adapted to the detection and quantification of antibody in serum specimens and support the use of these tests for the disease surveillance and disease prevention research in feedlot cattle. |
| first_indexed | 2024-03-11T04:25:27Z |
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| issn | 2076-2615 |
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| last_indexed | 2024-03-11T04:25:27Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-ba48057b5f41433fbe3bfc8b95467d732023-11-17T22:30:22ZengMDPI AGAnimals2076-26152023-05-01139153110.3390/ani13091531Detection of <i>Mannheimia haemolytica</i>-Specific IgG, IgM and IgA in Sera and Their Relationship to Respiratory Disease in CattleKorakrit Poonsuk0Carita Kordik1Matthew Hille2Ting-Yu Cheng3William B. Crosby4Amelia R. Woolums5Michael L. Clawson6Carol Chitko-McKown7Bruce Brodersen8John Dustin Loy9Nebraska Veterinary Diagnostic Center, School of Veterinary Medicine and Biomedical Sciences, University of Nebraska–Lincoln, Lincoln, NE 68503, USANebraska Veterinary Diagnostic Center, School of Veterinary Medicine and Biomedical Sciences, University of Nebraska–Lincoln, Lincoln, NE 68503, USANebraska Veterinary Diagnostic Center, School of Veterinary Medicine and Biomedical Sciences, University of Nebraska–Lincoln, Lincoln, NE 68503, USADepartment of Veterinary Preventive Medicine, College of Veterinary Medicine, The Ohio State University, Columbus, OH 43210, USADepartment of Pathobiology and Population Medicine, Mississippi State University, Mississippi State, MS 39762, USADepartment of Pathobiology and Population Medicine, Mississippi State University, Mississippi State, MS 39762, USAUnited States Department of Agriculture (USDA), Agricultural Research Service (ARS), United States Meat Animal Research Center, Clay Center, NE 68933, USAUnited States Department of Agriculture (USDA), Agricultural Research Service (ARS), United States Meat Animal Research Center, Clay Center, NE 68933, USANebraska Veterinary Diagnostic Center, School of Veterinary Medicine and Biomedical Sciences, University of Nebraska–Lincoln, Lincoln, NE 68503, USANebraska Veterinary Diagnostic Center, School of Veterinary Medicine and Biomedical Sciences, University of Nebraska–Lincoln, Lincoln, NE 68503, USA<i>Mannheimia haemolytica</i> is one of the major causes of bovine respiratory disease in cattle. The organism is the primary bacterium isolated from calves and young cattle affected with enzootic pneumonia. Novel indirect ELISAs were developed and evaluated to enable quantification of antibody responses to whole cell antigens using <i>M. haemolytica</i> A1 strain P1148. In this study, the ELISAs were initially developed using sera from both <i>M. haemolytica</i>-culture-free and clinically infected cattle, then the final prototypes were tested in the validation phase using a larger set of known-status <i>M. haemolytica</i> sera (<i>n</i> = 145) collected from feedlot cattle. The test showed good inter-assay and intra-assay repeatability. Diagnostic sensitivity and specificity were estimated at 91% and 87% for IgG at a cutoff of S/P ≥ 0.8. IgM diagnostic sensitivity and specificity were 91% and 81% at a cutoff of sample to positive (S/P) ratio ≥ 0.8. IgA diagnostic sensitivity was 89% whereas specificity was 78% at a cutoff of S/P ≥ 0.2. ELISA results of all isotypes were related to the diagnosis of respiratory disease and isolation of <i>M. haemolytica</i> (<i>p</i>-value < 0.05). These data suggest that <i>M. haemolytica</i> ELISAs can be adapted to the detection and quantification of antibody in serum specimens and support the use of these tests for the disease surveillance and disease prevention research in feedlot cattle.https://www.mdpi.com/2076-2615/13/9/1531<i>Mannheimia haemolytica</i>bovine respiratory diseaseantibodyELISAIgGIgM |
| spellingShingle | Korakrit Poonsuk Carita Kordik Matthew Hille Ting-Yu Cheng William B. Crosby Amelia R. Woolums Michael L. Clawson Carol Chitko-McKown Bruce Brodersen John Dustin Loy Detection of <i>Mannheimia haemolytica</i>-Specific IgG, IgM and IgA in Sera and Their Relationship to Respiratory Disease in Cattle Animals <i>Mannheimia haemolytica</i> bovine respiratory disease antibody ELISA IgG IgM |
| title | Detection of <i>Mannheimia haemolytica</i>-Specific IgG, IgM and IgA in Sera and Their Relationship to Respiratory Disease in Cattle |
| title_full | Detection of <i>Mannheimia haemolytica</i>-Specific IgG, IgM and IgA in Sera and Their Relationship to Respiratory Disease in Cattle |
| title_fullStr | Detection of <i>Mannheimia haemolytica</i>-Specific IgG, IgM and IgA in Sera and Their Relationship to Respiratory Disease in Cattle |
| title_full_unstemmed | Detection of <i>Mannheimia haemolytica</i>-Specific IgG, IgM and IgA in Sera and Their Relationship to Respiratory Disease in Cattle |
| title_short | Detection of <i>Mannheimia haemolytica</i>-Specific IgG, IgM and IgA in Sera and Their Relationship to Respiratory Disease in Cattle |
| title_sort | detection of i mannheimia haemolytica i specific igg igm and iga in sera and their relationship to respiratory disease in cattle |
| topic | <i>Mannheimia haemolytica</i> bovine respiratory disease antibody ELISA IgG IgM |
| url | https://www.mdpi.com/2076-2615/13/9/1531 |
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