Role of Extended Spectrum Beta Lactamases in Cephalosporin and Carbapenem Resistance in Escherichia coli from Inpatients and Outpatients in Nigeria
Introduction: Antimicrobial resistance requires global coordinated action with a view to addressing its rising threat. Prevalence of antimicrobial resistance has been increasing worldwide in both developed and developing countries. Whilst cephalosporin and carbapenem resistance is a problem in Esche...
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JCDR Research and Publications Private Limited
2020-02-01
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| Series: | Journal of Clinical and Diagnostic Research |
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| Online Access: | https://jcdr.net/articles/PDF/13515/43328_F(SL)_PF1(AG_SHU)_PFA(KM)_PB(AG_KM)_PN(SL).pdf |
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| author | Akinwale Majeed Akinlabi Afolabi Oluwadun Oyebode Armstrong Terry Alli Adeolu Sunday Oluremi Mark A Webber David Olusoga Ogbolu |
| author_facet | Akinwale Majeed Akinlabi Afolabi Oluwadun Oyebode Armstrong Terry Alli Adeolu Sunday Oluremi Mark A Webber David Olusoga Ogbolu |
| author_sort | Akinwale Majeed Akinlabi |
| collection | DOAJ |
| description | Introduction: Antimicrobial resistance requires global coordinated action with a view to addressing its rising threat. Prevalence of antimicrobial resistance has been increasing worldwide in both developed and developing countries. Whilst cephalosporin and carbapenem resistance is a problem in Escherichia coli in Nigeria, there is a paucity of information regarding the mechanisms of resistance underpinning this in prevalence and types of extended spectrum in clinical E. coli. Aim: To detect and characterise Extended Spectrum β Lactamases (ESBL) in clinical E. coli from inpatients and outpatients in Nigeria. Materials and Methods: A total of 104 E. coli were obtained from 498 non-duplicate clinical specimens from Northern Nigeria between November 2017 and November 2018. Antibiotic susceptibility of third generation cephalosporin including other important antibiotics and phenotypic detection of ESBL of the isolates were determined. Genotypic detection of ESBL and randomly amplified polymorphic DNA to determine clonality were used to further characterise 44 E. coli isolates selected based on their phenotypes and clinical specimens. The data were analysed with the aid of statistical package for social sciences (IBM SPSS), version 21.0 and were reported in frequency tables and in percentages. Results: Majority of the E. coli isolates showed no clonal relationships. More than half of E. coli were resistant to third generation cephalosporin class of antibiotics. There was no difference between MIC50 and MIC90 values for the majority isolates for most drugs where MICs ≥256 µg/mL were the norm except for carbapenems with low level resistance. In total, 44/104 (42.3%) E. coli were ESBL producers. blaCTX-M was the dominant ESBLs seen in 75% (33/44) of isolates, of these blaCTX-M-15 variant was most common and seen in 72.7% (24/33) of isolates followed by blaVEB, 21/44 (47.7%) and blaPER 6/44 (13.6%). No AmpC or carbapenemase genes were identified. Conclusion: E. coli isolates from Northern Nigeria are highly multi-drug resistant with only carbapenems of common therapeutic drug classes retaining significant activity. Betalactam resistance is largely underpinned by carriage of CTX-M15 and carbapenem resistance is likely to be a result of ESBL carriage with other mechanisms. |
| first_indexed | 2024-12-16T06:58:32Z |
| format | Article |
| id | doaj.art-ba488945c9af4b379cadca8ba650a71e |
| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-12-16T06:58:32Z |
| publishDate | 2020-02-01 |
| publisher | JCDR Research and Publications Private Limited |
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| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-ba488945c9af4b379cadca8ba650a71e2022-12-21T22:40:14ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2020-02-01142DC10DC1510.7860/JCDR/2020/43328.13515Role of Extended Spectrum Beta Lactamases in Cephalosporin and Carbapenem Resistance in Escherichia coli from Inpatients and Outpatients in NigeriaAkinwale Majeed Akinlabi0Afolabi Oluwadun1Oyebode Armstrong Terry Alli2Adeolu Sunday Oluremi3Mark A Webber4David Olusoga Ogbolu5Research Assistant, Department of Medical Microbiology and Parasitology, Olabisi Onabanjo University, Ago-Iwoye, Ogun, Nigeria.Professor, Department of Medical Microbiology and Parasitology, Olabisi Onabanjo University, Ago-Iwoye, Ogun, Nigeria.Professor, Department of Biomedical Science, Ladoke Akintola University of Technology, Ogbomoso, Oyo, Nigeria.Research Assistant, Department of Biomedical Science, Ladoke Akintola University of Technology, Ogbomoso, Oyo, Nigeria.Research Group Leader, Department of Microbes in the Food Chain, Quadram Institute Bioscience, Norwich, England, United Kingdom.Reader, Department of Biomedical Science, Ladoke Akintola University of Technology, Ogbomoso, Oyo, Nigeria.Introduction: Antimicrobial resistance requires global coordinated action with a view to addressing its rising threat. Prevalence of antimicrobial resistance has been increasing worldwide in both developed and developing countries. Whilst cephalosporin and carbapenem resistance is a problem in Escherichia coli in Nigeria, there is a paucity of information regarding the mechanisms of resistance underpinning this in prevalence and types of extended spectrum in clinical E. coli. Aim: To detect and characterise Extended Spectrum β Lactamases (ESBL) in clinical E. coli from inpatients and outpatients in Nigeria. Materials and Methods: A total of 104 E. coli were obtained from 498 non-duplicate clinical specimens from Northern Nigeria between November 2017 and November 2018. Antibiotic susceptibility of third generation cephalosporin including other important antibiotics and phenotypic detection of ESBL of the isolates were determined. Genotypic detection of ESBL and randomly amplified polymorphic DNA to determine clonality were used to further characterise 44 E. coli isolates selected based on their phenotypes and clinical specimens. The data were analysed with the aid of statistical package for social sciences (IBM SPSS), version 21.0 and were reported in frequency tables and in percentages. Results: Majority of the E. coli isolates showed no clonal relationships. More than half of E. coli were resistant to third generation cephalosporin class of antibiotics. There was no difference between MIC50 and MIC90 values for the majority isolates for most drugs where MICs ≥256 µg/mL were the norm except for carbapenems with low level resistance. In total, 44/104 (42.3%) E. coli were ESBL producers. blaCTX-M was the dominant ESBLs seen in 75% (33/44) of isolates, of these blaCTX-M-15 variant was most common and seen in 72.7% (24/33) of isolates followed by blaVEB, 21/44 (47.7%) and blaPER 6/44 (13.6%). No AmpC or carbapenemase genes were identified. Conclusion: E. coli isolates from Northern Nigeria are highly multi-drug resistant with only carbapenems of common therapeutic drug classes retaining significant activity. Betalactam resistance is largely underpinned by carriage of CTX-M15 and carbapenem resistance is likely to be a result of ESBL carriage with other mechanisms.https://jcdr.net/articles/PDF/13515/43328_F(SL)_PF1(AG_SHU)_PFA(KM)_PB(AG_KM)_PN(SL).pdfantibioticsbacteriainfectionsresistance genes |
| spellingShingle | Akinwale Majeed Akinlabi Afolabi Oluwadun Oyebode Armstrong Terry Alli Adeolu Sunday Oluremi Mark A Webber David Olusoga Ogbolu Role of Extended Spectrum Beta Lactamases in Cephalosporin and Carbapenem Resistance in Escherichia coli from Inpatients and Outpatients in Nigeria Journal of Clinical and Diagnostic Research antibiotics bacteria infections resistance genes |
| title | Role of Extended Spectrum Beta Lactamases in Cephalosporin and Carbapenem Resistance in Escherichia coli from Inpatients and Outpatients in Nigeria |
| title_full | Role of Extended Spectrum Beta Lactamases in Cephalosporin and Carbapenem Resistance in Escherichia coli from Inpatients and Outpatients in Nigeria |
| title_fullStr | Role of Extended Spectrum Beta Lactamases in Cephalosporin and Carbapenem Resistance in Escherichia coli from Inpatients and Outpatients in Nigeria |
| title_full_unstemmed | Role of Extended Spectrum Beta Lactamases in Cephalosporin and Carbapenem Resistance in Escherichia coli from Inpatients and Outpatients in Nigeria |
| title_short | Role of Extended Spectrum Beta Lactamases in Cephalosporin and Carbapenem Resistance in Escherichia coli from Inpatients and Outpatients in Nigeria |
| title_sort | role of extended spectrum beta lactamases in cephalosporin and carbapenem resistance in escherichia coli from inpatients and outpatients in nigeria |
| topic | antibiotics bacteria infections resistance genes |
| url | https://jcdr.net/articles/PDF/13515/43328_F(SL)_PF1(AG_SHU)_PFA(KM)_PB(AG_KM)_PN(SL).pdf |
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