<i>Dnd1</i> Knockout in Sturgeons By CRISPR/Cas9 Generates Germ Cell Free Host for Surrogate Production
Sturgeons also known as living fossils are facing threats to their survival due to overfishing and interference in natural habitats. Sterlet (<i>Acipenser ruthenus</i>) due to its rapid reproductive cycle and small body size can be used as a sterile host for surrogate production for late...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-04-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/9/4/174 |
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| author | Abdul Rasheed Baloch Roman Franěk Tomáš Tichopád Michaela Fučíková Marek Rodina Martin Pšenička |
| author_facet | Abdul Rasheed Baloch Roman Franěk Tomáš Tichopád Michaela Fučíková Marek Rodina Martin Pšenička |
| author_sort | Abdul Rasheed Baloch |
| collection | DOAJ |
| description | Sturgeons also known as living fossils are facing threats to their survival due to overfishing and interference in natural habitats. Sterlet (<i>Acipenser ruthenus</i>) due to its rapid reproductive cycle and small body size can be used as a sterile host for surrogate production for late maturing and large sturgeon species. Dead end protein (dnd1) is essential for migration of Primordial Germ Cells (PGCs), the origin of all germ cells in developing embryos. Knockout or knockdown of <i>dnd1</i> can be done in order to mismigrate PGCs. Previously we have used MO and UV for the aforementioned purpose, and in our present study we have used CRISPR/Cas9 technology to knockout <i>dnd1</i>. No or a smaller number of PGCs were detected in crispants, and we also observed malformations in some CRISPR/Cas9 injected embryos. Furthermore, we compared three established methods to achieve sterility in sterlet, and we found higher embryo survival and hatching rates in CRISPR/Cas9, UV and MO, respectively. |
| first_indexed | 2024-04-11T22:55:43Z |
| format | Article |
| id | doaj.art-ba489fb5aea642e694213250cc8f3c33 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-04-11T22:55:43Z |
| publishDate | 2019-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-ba489fb5aea642e694213250cc8f3c332022-12-22T03:58:25ZengMDPI AGAnimals2076-26152019-04-019417410.3390/ani9040174ani9040174<i>Dnd1</i> Knockout in Sturgeons By CRISPR/Cas9 Generates Germ Cell Free Host for Surrogate ProductionAbdul Rasheed Baloch0Roman Franěk1Tomáš Tichopád2Michaela Fučíková3Marek Rodina4Martin Pšenička5Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, University of South Bohemia in Ceske Budejovice, Zátiší 728/II, 389 25 Vodňany, Czech RepublicFaculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, University of South Bohemia in Ceske Budejovice, Zátiší 728/II, 389 25 Vodňany, Czech RepublicFaculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, University of South Bohemia in Ceske Budejovice, Zátiší 728/II, 389 25 Vodňany, Czech RepublicFaculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, University of South Bohemia in Ceske Budejovice, Zátiší 728/II, 389 25 Vodňany, Czech RepublicFaculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, University of South Bohemia in Ceske Budejovice, Zátiší 728/II, 389 25 Vodňany, Czech RepublicFaculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, University of South Bohemia in Ceske Budejovice, Zátiší 728/II, 389 25 Vodňany, Czech RepublicSturgeons also known as living fossils are facing threats to their survival due to overfishing and interference in natural habitats. Sterlet (<i>Acipenser ruthenus</i>) due to its rapid reproductive cycle and small body size can be used as a sterile host for surrogate production for late maturing and large sturgeon species. Dead end protein (dnd1) is essential for migration of Primordial Germ Cells (PGCs), the origin of all germ cells in developing embryos. Knockout or knockdown of <i>dnd1</i> can be done in order to mismigrate PGCs. Previously we have used MO and UV for the aforementioned purpose, and in our present study we have used CRISPR/Cas9 technology to knockout <i>dnd1</i>. No or a smaller number of PGCs were detected in crispants, and we also observed malformations in some CRISPR/Cas9 injected embryos. Furthermore, we compared three established methods to achieve sterility in sterlet, and we found higher embryo survival and hatching rates in CRISPR/Cas9, UV and MO, respectively.https://www.mdpi.com/2076-2615/9/4/174Acipensercaviarconservationgenome editingmorpholino oligonucleotidePGCs |
| spellingShingle | Abdul Rasheed Baloch Roman Franěk Tomáš Tichopád Michaela Fučíková Marek Rodina Martin Pšenička <i>Dnd1</i> Knockout in Sturgeons By CRISPR/Cas9 Generates Germ Cell Free Host for Surrogate Production Animals Acipenser caviar conservation genome editing morpholino oligonucleotide PGCs |
| title | <i>Dnd1</i> Knockout in Sturgeons By CRISPR/Cas9 Generates Germ Cell Free Host for Surrogate Production |
| title_full | <i>Dnd1</i> Knockout in Sturgeons By CRISPR/Cas9 Generates Germ Cell Free Host for Surrogate Production |
| title_fullStr | <i>Dnd1</i> Knockout in Sturgeons By CRISPR/Cas9 Generates Germ Cell Free Host for Surrogate Production |
| title_full_unstemmed | <i>Dnd1</i> Knockout in Sturgeons By CRISPR/Cas9 Generates Germ Cell Free Host for Surrogate Production |
| title_short | <i>Dnd1</i> Knockout in Sturgeons By CRISPR/Cas9 Generates Germ Cell Free Host for Surrogate Production |
| title_sort | i dnd1 i knockout in sturgeons by crispr cas9 generates germ cell free host for surrogate production |
| topic | Acipenser caviar conservation genome editing morpholino oligonucleotide PGCs |
| url | https://www.mdpi.com/2076-2615/9/4/174 |
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