Alterations in gene expression profiles correlated with cisplatin cytotoxicity in the glioma U343 cell line
Gliomas are the most common tumors in the central nervous system, the average survival time of patients with glioblastoma multiforme being about 1 year from diagnosis, in spite of harsh therapy. Aiming to study the transcriptional profiles displayed by glioma cells undergoing cisplatin treatment, ge...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Genética
2010-01-01
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| Series: | Genetics and Molecular Biology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100027 |
| _version_ | 1818334430393008128 |
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| author | Patricia Oliveira Carminati Stephano Spano Mello Ana Lucia Fachin Cristina Moraes Junta Paula Sandrin-Garcia Carlos Gilberto Carlotti Eduardo Antonio Donadi Geraldo Aleixo Silva Passos Elza Tiemi Sakamoto-Hojo |
| author_facet | Patricia Oliveira Carminati Stephano Spano Mello Ana Lucia Fachin Cristina Moraes Junta Paula Sandrin-Garcia Carlos Gilberto Carlotti Eduardo Antonio Donadi Geraldo Aleixo Silva Passos Elza Tiemi Sakamoto-Hojo |
| author_sort | Patricia Oliveira Carminati |
| collection | DOAJ |
| description | Gliomas are the most common tumors in the central nervous system, the average survival time of patients with glioblastoma multiforme being about 1 year from diagnosis, in spite of harsh therapy. Aiming to study the transcriptional profiles displayed by glioma cells undergoing cisplatin treatment, gene expression analysis was performed by the cDNA microarray method. Cell survival and apoptosis induction following treatment were also evaluated. Drug concentrations of 12.5 to 300 μM caused a pronounced reduction in cell survival rates five days after treatment, whereas concentrations higher than 25 μM were effective in reducing the survival rates to ~1%. However, the maximum apoptosis frequency was 20.4% for 25 μM cisplatin in cells analyzed at 72 h, indicating that apoptosis is not the only kind of cell death induced by cisplatin. An analysis of gene expression revealed 67 significantly (FDR < 0.05) modulated genes: 29 of which down- and 38 up-regulated. These genes belong to several classes (metabolism, protein localization, cell proliferation, apoptosis, adhesion, stress response, cell cycle and DNA repair) that may represent several affected cell processes under the influence of cisplatin treatment. The expression pattern of three genes (RHOA, LIMK2 and TIMP2) was confirmed by the real time PCR method. |
| first_indexed | 2024-12-13T14:07:24Z |
| format | Article |
| id | doaj.art-ba4a5ee8d787421689ca08c89892200f |
| institution | Directory Open Access Journal |
| issn | 1415-4757 1678-4685 |
| language | English |
| last_indexed | 2024-12-13T14:07:24Z |
| publishDate | 2010-01-01 |
| publisher | Sociedade Brasileira de Genética |
| record_format | Article |
| series | Genetics and Molecular Biology |
| spelling | doaj.art-ba4a5ee8d787421689ca08c89892200f2022-12-21T23:42:34ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1415-47571678-46852010-01-01331159168Alterations in gene expression profiles correlated with cisplatin cytotoxicity in the glioma U343 cell linePatricia Oliveira CarminatiStephano Spano MelloAna Lucia FachinCristina Moraes JuntaPaula Sandrin-GarciaCarlos Gilberto CarlottiEduardo Antonio DonadiGeraldo Aleixo Silva PassosElza Tiemi Sakamoto-HojoGliomas are the most common tumors in the central nervous system, the average survival time of patients with glioblastoma multiforme being about 1 year from diagnosis, in spite of harsh therapy. Aiming to study the transcriptional profiles displayed by glioma cells undergoing cisplatin treatment, gene expression analysis was performed by the cDNA microarray method. Cell survival and apoptosis induction following treatment were also evaluated. Drug concentrations of 12.5 to 300 μM caused a pronounced reduction in cell survival rates five days after treatment, whereas concentrations higher than 25 μM were effective in reducing the survival rates to ~1%. However, the maximum apoptosis frequency was 20.4% for 25 μM cisplatin in cells analyzed at 72 h, indicating that apoptosis is not the only kind of cell death induced by cisplatin. An analysis of gene expression revealed 67 significantly (FDR < 0.05) modulated genes: 29 of which down- and 38 up-regulated. These genes belong to several classes (metabolism, protein localization, cell proliferation, apoptosis, adhesion, stress response, cell cycle and DNA repair) that may represent several affected cell processes under the influence of cisplatin treatment. The expression pattern of three genes (RHOA, LIMK2 and TIMP2) was confirmed by the real time PCR method.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100027apoptosiscisplatingene expressionglioma |
| spellingShingle | Patricia Oliveira Carminati Stephano Spano Mello Ana Lucia Fachin Cristina Moraes Junta Paula Sandrin-Garcia Carlos Gilberto Carlotti Eduardo Antonio Donadi Geraldo Aleixo Silva Passos Elza Tiemi Sakamoto-Hojo Alterations in gene expression profiles correlated with cisplatin cytotoxicity in the glioma U343 cell line Genetics and Molecular Biology apoptosis cisplatin gene expression glioma |
| title | Alterations in gene expression profiles correlated with cisplatin cytotoxicity in the glioma U343 cell line |
| title_full | Alterations in gene expression profiles correlated with cisplatin cytotoxicity in the glioma U343 cell line |
| title_fullStr | Alterations in gene expression profiles correlated with cisplatin cytotoxicity in the glioma U343 cell line |
| title_full_unstemmed | Alterations in gene expression profiles correlated with cisplatin cytotoxicity in the glioma U343 cell line |
| title_short | Alterations in gene expression profiles correlated with cisplatin cytotoxicity in the glioma U343 cell line |
| title_sort | alterations in gene expression profiles correlated with cisplatin cytotoxicity in the glioma u343 cell line |
| topic | apoptosis cisplatin gene expression glioma |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100027 |
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