Protocol to longitudinally quantify SARS-CoV-2 RNA in wastewater using RT-qPCR and pepper mild mottle virus normalization

Protocol to longitudinally quantify SARS-CoV-2 RNA in wastewater using RT-qPCR and pepper mild mottle virus normalization

Summary: Wastewater surveillance allows severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection levels to be tracked in a community. Here, we present a protocol to longitudinally quantify SARS-CoV-2 RNA in wastewater using quantitative reverse-transcription PCR (RT-qPCR) and pepper mi...

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Bibliographic Details
Main Authors: Bryan Sanchez Jimenez, Trinity Sterling, Austin Brown, Brian Modica, Kaylee Gibson, Hannah Collins, Carolyn Koch, Tyler Schwarz, Kristine N. Dye
Format: Article
Language:English
Published: Elsevier 2024-06-01
Series:STAR Protocols
Subjects:
Environmental sciences
Microbiology
Molecular Biology
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166724001667

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