Molecular diagnostics for verification of pleural tuberculosis in Morocco
Pleural tuberculosis (pTB) is a very common form of extrapulmonary tuberculosis (TB). pTB diagnostics represents a major burning challenge worldwide due to the limitations of available conventional diagnostic tools. These latter include microscopic examination of the pleural fluid for acid-fast baci...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | Russian |
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Sankt-Peterburg : NIIÈM imeni Pastera
2021-06-01
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| Series: | Инфекция и иммунитет |
| Subjects: | |
| Online Access: | https://www.iimmun.ru/iimm/article/view/1399 |
| _version_ | 1828082800653238272 |
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| author | I. Chaoui S. Taoudi A. Oudghiri J. Benamor J. Bourkadi M. El Mzibri |
| author_facet | I. Chaoui S. Taoudi A. Oudghiri J. Benamor J. Bourkadi M. El Mzibri |
| author_sort | I. Chaoui |
| collection | DOAJ |
| description | Pleural tuberculosis (pTB) is a very common form of extrapulmonary tuberculosis (TB). pTB diagnostics represents a major burning challenge worldwide due to the limitations of available conventional diagnostic tools. These latter include microscopic examination of the pleural fluid for acid-fast bacilli, mycobacterial culture of pleural fluid in solid or liquid media, sputum or pleural tissue, and histopathological examination of pleural tissue; these tests have recognized limitations for clinical use. Hence, to overcome these limitations, attention has been devoted to new nucleic acid amplification (NAA) diagnostic tests such as the polymerase chain reaction (PCR) and real-time PCR (RT-PCR), owing to their accuracy, rapidity, high sensitivity and specificity. Within this context, this prospective study was conducted to evaluate the performance of molecular diagnosis methods for differentiation between tuberculosis and non-tuberculosis pleural effusions. Fifty patients with pleural effusion were enrolled in this prospective study in Rabat, Morocco. The efficacy of conventional polymerase chain reaction (PCR) in the diagnostics of tuberculous pleurisy by targeting IS6110 and mycobacterial internal transcribed spacer (MYITS) was evaluated compared to histopathologic examination and culture data. Our results showed that IS6110 PCR could “rule in” pTB, the sensitivity and specificity being 41.6% and 85.7%, respectively. Therefore, the findings confirmed that molecular tests exert a relatively high specificity in EPTB but lower sensitivity, thus a positive test is considered as a pTB case whereas negative one cannot exclude the disease. Although the study was limited by a small sample size, it adds to the body of evidence of usefulness of molecular testing as adjuncts to histopathologic examination for accurate diagnosis of pTB, to treat timely and to avoid the emergence and spread of drug resistant pTB. However, further efforts should be made to increase the sensitivity of NAA methods and to identify the best molecular targets to be useful in clinical practice. |
| first_indexed | 2024-04-11T03:58:48Z |
| format | Article |
| id | doaj.art-ba4e787f7bc94d0693eb7f61290db186 |
| institution | Directory Open Access Journal |
| issn | 2220-7619 2313-7398 |
| language | Russian |
| last_indexed | 2024-04-11T03:58:48Z |
| publishDate | 2021-06-01 |
| publisher | Sankt-Peterburg : NIIÈM imeni Pastera |
| record_format | Article |
| series | Инфекция и иммунитет |
| spelling | doaj.art-ba4e787f7bc94d0693eb7f61290db1862023-01-02T00:08:14ZrusSankt-Peterburg : NIIÈM imeni PasteraИнфекция и иммунитет2220-76192313-73982021-06-0111349149610.15789/2220-7619-MDO-1399955Molecular diagnostics for verification of pleural tuberculosis in MoroccoI. Chaoui0S. Taoudi1A. Oudghiri2J. Benamor3J. Bourkadi4M. El Mzibri5National Center for Energy, Sciences and Nuclear TechniquesMoulay Youssef HospitalNational Center for Energy, Sciences and Nuclear TechniquesMoulay Youssef HospitalMoulay Youssef HospitalNational Center for Energy, Sciences and Nuclear TechniquesPleural tuberculosis (pTB) is a very common form of extrapulmonary tuberculosis (TB). pTB diagnostics represents a major burning challenge worldwide due to the limitations of available conventional diagnostic tools. These latter include microscopic examination of the pleural fluid for acid-fast bacilli, mycobacterial culture of pleural fluid in solid or liquid media, sputum or pleural tissue, and histopathological examination of pleural tissue; these tests have recognized limitations for clinical use. Hence, to overcome these limitations, attention has been devoted to new nucleic acid amplification (NAA) diagnostic tests such as the polymerase chain reaction (PCR) and real-time PCR (RT-PCR), owing to their accuracy, rapidity, high sensitivity and specificity. Within this context, this prospective study was conducted to evaluate the performance of molecular diagnosis methods for differentiation between tuberculosis and non-tuberculosis pleural effusions. Fifty patients with pleural effusion were enrolled in this prospective study in Rabat, Morocco. The efficacy of conventional polymerase chain reaction (PCR) in the diagnostics of tuberculous pleurisy by targeting IS6110 and mycobacterial internal transcribed spacer (MYITS) was evaluated compared to histopathologic examination and culture data. Our results showed that IS6110 PCR could “rule in” pTB, the sensitivity and specificity being 41.6% and 85.7%, respectively. Therefore, the findings confirmed that molecular tests exert a relatively high specificity in EPTB but lower sensitivity, thus a positive test is considered as a pTB case whereas negative one cannot exclude the disease. Although the study was limited by a small sample size, it adds to the body of evidence of usefulness of molecular testing as adjuncts to histopathologic examination for accurate diagnosis of pTB, to treat timely and to avoid the emergence and spread of drug resistant pTB. However, further efforts should be made to increase the sensitivity of NAA methods and to identify the best molecular targets to be useful in clinical practice.https://www.iimmun.ru/iimm/article/view/1399туберкулезный плевритmycobacterium tuberculosisполимеразная цепная реакцияis6110внутренний транскрибируемый спейсер микобактерий (myits) |
| spellingShingle | I. Chaoui S. Taoudi A. Oudghiri J. Benamor J. Bourkadi M. El Mzibri Molecular diagnostics for verification of pleural tuberculosis in Morocco Инфекция и иммунитет туберкулезный плеврит mycobacterium tuberculosis полимеразная цепная реакция is6110 внутренний транскрибируемый спейсер микобактерий (myits) |
| title | Molecular diagnostics for verification of pleural tuberculosis in Morocco |
| title_full | Molecular diagnostics for verification of pleural tuberculosis in Morocco |
| title_fullStr | Molecular diagnostics for verification of pleural tuberculosis in Morocco |
| title_full_unstemmed | Molecular diagnostics for verification of pleural tuberculosis in Morocco |
| title_short | Molecular diagnostics for verification of pleural tuberculosis in Morocco |
| title_sort | molecular diagnostics for verification of pleural tuberculosis in morocco |
| topic | туберкулезный плеврит mycobacterium tuberculosis полимеразная цепная реакция is6110 внутренний транскрибируемый спейсер микобактерий (myits) |
| url | https://www.iimmun.ru/iimm/article/view/1399 |
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