Identification of Glucose Metabolism-Related Genes in the Progression from Nonalcoholic Fatty Liver Disease to Hepatocellular Carcinoma
Nonalcoholic fatty liver disease (NAFLD) is a manifestation of hepatic metabolic syndrome that varies in severity. Hepatocellular carcinoma progresses from NAFLD when there is heterogeneity in the infiltration of immune cells and molecules. A precise molecular classification of NAFLD remains lacking...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Hindawi - Cambridge University Press
2022-01-01
|
| Series: | Genetics Research |
| Online Access: | http://dx.doi.org/10.1155/2022/8566342 |
| _version_ | 1811180255854985216 |
|---|---|
| author | Siyuan Wang Yiling Li Ning Liu Wei Shen Wenhao Xu Peng Yao |
| author_facet | Siyuan Wang Yiling Li Ning Liu Wei Shen Wenhao Xu Peng Yao |
| author_sort | Siyuan Wang |
| collection | DOAJ |
| description | Nonalcoholic fatty liver disease (NAFLD) is a manifestation of hepatic metabolic syndrome that varies in severity. Hepatocellular carcinoma progresses from NAFLD when there is heterogeneity in the infiltration of immune cells and molecules. A precise molecular classification of NAFLD remains lacking, allowing further exploration of the link between NAFLD and hepatocellular carcinoma. In this work, a weighted gene coexpression network analysis was used to identify two coexpression modules based on multiple omics data used to differentiate NAFLD subtypes. Additionally, key genes in the process of glucose metabolism and NAFLD were used to construct a prognostic model in a cohort of patients with hepatocellular carcinoma. Furthermore, the specific expression of signature genes in hepatocellular carcinoma cells was analyzed using a single-cell RNA sequencing approach. A total of 19 liver tissues of NAFLD patients were obtained from the GEO database, and 81 glucose metabolism-related genes were downloaded from the CTD database. In addition, based on nine signature genes, we constructed a prognostic model to divide the HCC cohort into high and low-risk groups. We also demonstrated a significant correlation between prognostic models and clinical phenotypes. Furthermore, we integrated single-cell RNA-sequencing data and immunology data to assess potential relationships between different molecular subtypes and hepatocellular carcinoma. Finally, our study discovered that the glucose metabolism pathway may play an important role in the process of NAFLD-hepatocellular carcinoma. In addition, three glucose metabolism-related genes (SERPINE1, VCAN, and TFPI2) may be the potential targets for the immunotherapy of patients with NAFLD-hepatocellular carcinoma. |
| first_indexed | 2024-04-11T06:48:18Z |
| format | Article |
| id | doaj.art-ba5b907802bd4c868f22e414baad57dd |
| institution | Directory Open Access Journal |
| issn | 1469-5073 |
| language | English |
| last_indexed | 2024-04-11T06:48:18Z |
| publishDate | 2022-01-01 |
| publisher | Hindawi - Cambridge University Press |
| record_format | Article |
| series | Genetics Research |
| spelling | doaj.art-ba5b907802bd4c868f22e414baad57dd2022-12-22T04:39:18ZengHindawi - Cambridge University PressGenetics Research1469-50732022-01-01202210.1155/2022/8566342Identification of Glucose Metabolism-Related Genes in the Progression from Nonalcoholic Fatty Liver Disease to Hepatocellular CarcinomaSiyuan Wang0Yiling Li1Ning Liu2Wei Shen3Wenhao Xu4Peng Yao5Department of Hepatobiliary SurgeryDepartment of AnesthesiologyDepartment of Hepatobiliary SurgeryDepartment of Intensive Care UnitDepartment of Intensive Care UnitDepartment of Intensive Care UnitNonalcoholic fatty liver disease (NAFLD) is a manifestation of hepatic metabolic syndrome that varies in severity. Hepatocellular carcinoma progresses from NAFLD when there is heterogeneity in the infiltration of immune cells and molecules. A precise molecular classification of NAFLD remains lacking, allowing further exploration of the link between NAFLD and hepatocellular carcinoma. In this work, a weighted gene coexpression network analysis was used to identify two coexpression modules based on multiple omics data used to differentiate NAFLD subtypes. Additionally, key genes in the process of glucose metabolism and NAFLD were used to construct a prognostic model in a cohort of patients with hepatocellular carcinoma. Furthermore, the specific expression of signature genes in hepatocellular carcinoma cells was analyzed using a single-cell RNA sequencing approach. A total of 19 liver tissues of NAFLD patients were obtained from the GEO database, and 81 glucose metabolism-related genes were downloaded from the CTD database. In addition, based on nine signature genes, we constructed a prognostic model to divide the HCC cohort into high and low-risk groups. We also demonstrated a significant correlation between prognostic models and clinical phenotypes. Furthermore, we integrated single-cell RNA-sequencing data and immunology data to assess potential relationships between different molecular subtypes and hepatocellular carcinoma. Finally, our study discovered that the glucose metabolism pathway may play an important role in the process of NAFLD-hepatocellular carcinoma. In addition, three glucose metabolism-related genes (SERPINE1, VCAN, and TFPI2) may be the potential targets for the immunotherapy of patients with NAFLD-hepatocellular carcinoma.http://dx.doi.org/10.1155/2022/8566342 |
| spellingShingle | Siyuan Wang Yiling Li Ning Liu Wei Shen Wenhao Xu Peng Yao Identification of Glucose Metabolism-Related Genes in the Progression from Nonalcoholic Fatty Liver Disease to Hepatocellular Carcinoma Genetics Research |
| title | Identification of Glucose Metabolism-Related Genes in the Progression from Nonalcoholic Fatty Liver Disease to Hepatocellular Carcinoma |
| title_full | Identification of Glucose Metabolism-Related Genes in the Progression from Nonalcoholic Fatty Liver Disease to Hepatocellular Carcinoma |
| title_fullStr | Identification of Glucose Metabolism-Related Genes in the Progression from Nonalcoholic Fatty Liver Disease to Hepatocellular Carcinoma |
| title_full_unstemmed | Identification of Glucose Metabolism-Related Genes in the Progression from Nonalcoholic Fatty Liver Disease to Hepatocellular Carcinoma |
| title_short | Identification of Glucose Metabolism-Related Genes in the Progression from Nonalcoholic Fatty Liver Disease to Hepatocellular Carcinoma |
| title_sort | identification of glucose metabolism related genes in the progression from nonalcoholic fatty liver disease to hepatocellular carcinoma |
| url | http://dx.doi.org/10.1155/2022/8566342 |
| work_keys_str_mv | AT siyuanwang identificationofglucosemetabolismrelatedgenesintheprogressionfromnonalcoholicfattyliverdiseasetohepatocellularcarcinoma AT yilingli identificationofglucosemetabolismrelatedgenesintheprogressionfromnonalcoholicfattyliverdiseasetohepatocellularcarcinoma AT ningliu identificationofglucosemetabolismrelatedgenesintheprogressionfromnonalcoholicfattyliverdiseasetohepatocellularcarcinoma AT weishen identificationofglucosemetabolismrelatedgenesintheprogressionfromnonalcoholicfattyliverdiseasetohepatocellularcarcinoma AT wenhaoxu identificationofglucosemetabolismrelatedgenesintheprogressionfromnonalcoholicfattyliverdiseasetohepatocellularcarcinoma AT pengyao identificationofglucosemetabolismrelatedgenesintheprogressionfromnonalcoholicfattyliverdiseasetohepatocellularcarcinoma |