DETERMINATION OF THE ORIGIN OF REGENERATED FLAX PLANTS IN ANTHER CULTURE
The possibility to diagnose the ploidy of cells producing regenerated plants in vitro in anther culture has been studied. It is shown by the example of oil flax that population analysis of regenerated plants for segregation on the basis of a marker trait with a simple genetic control can serve as su...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2014-12-01
|
| Series: | Вавиловский журнал генетики и селекции |
| Subjects: | |
| Online Access: | https://vavilov.elpub.ru/jour/article/view/156 |
| _version_ | 1797214242903949312 |
|---|---|
| author | A. I. Soroka V. A. Lyakh |
| author_facet | A. I. Soroka V. A. Lyakh |
| author_sort | A. I. Soroka |
| collection | DOAJ |
| description | The possibility to diagnose the ploidy of cells producing regenerated plants in vitro in anther culture has been studied. It is shown by the example of oil flax that population analysis of regenerated plants for segregation on the basis of a marker trait with a simple genetic control can serve as such a method. When microspores are the source of regenerants, segregation of regenerants for a marker that coincides with the segregation at the gamete level is observed. If sporophytic tissues are the source for regenerated plants, there is no segregation. The proposed approach can be applied not only to anther cultures but also to ovule cultures, as well as to any plant species where relevant structures are used to obtain doubled haploids. |
| first_indexed | 2024-03-07T16:08:26Z |
| format | Article |
| id | doaj.art-ba5c7a2132464ce1b31bb10065dd986f |
| institution | Directory Open Access Journal |
| issn | 2500-3259 |
| language | English |
| last_indexed | 2024-04-24T11:11:04Z |
| publishDate | 2014-12-01 |
| publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
| record_format | Article |
| series | Вавиловский журнал генетики и селекции |
| spelling | doaj.art-ba5c7a2132464ce1b31bb10065dd986f2024-04-11T15:30:53ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592014-12-01172343348140DETERMINATION OF THE ORIGIN OF REGENERATED FLAX PLANTS IN ANTHER CULTUREA. I. Soroka0V. A. Lyakh1Institute of Oilseed Crops, National Academy of Agricultural Sciences of Ukraine, Zaporozhye, UkraineZaporozhye National University, Zaporozhye, UkraineThe possibility to diagnose the ploidy of cells producing regenerated plants in vitro in anther culture has been studied. It is shown by the example of oil flax that population analysis of regenerated plants for segregation on the basis of a marker trait with a simple genetic control can serve as such a method. When microspores are the source of regenerants, segregation of regenerants for a marker that coincides with the segregation at the gamete level is observed. If sporophytic tissues are the source for regenerated plants, there is no segregation. The proposed approach can be applied not only to anther cultures but also to ovule cultures, as well as to any plant species where relevant structures are used to obtain doubled haploids.https://vavilov.elpub.ru/jour/article/view/156ploidyanther culturedoubled haploidsegregationmarker traitoil flax |
| spellingShingle | A. I. Soroka V. A. Lyakh DETERMINATION OF THE ORIGIN OF REGENERATED FLAX PLANTS IN ANTHER CULTURE Вавиловский журнал генетики и селекции ploidy anther culture doubled haploid segregation marker trait oil flax |
| title | DETERMINATION OF THE ORIGIN OF REGENERATED FLAX PLANTS IN ANTHER CULTURE |
| title_full | DETERMINATION OF THE ORIGIN OF REGENERATED FLAX PLANTS IN ANTHER CULTURE |
| title_fullStr | DETERMINATION OF THE ORIGIN OF REGENERATED FLAX PLANTS IN ANTHER CULTURE |
| title_full_unstemmed | DETERMINATION OF THE ORIGIN OF REGENERATED FLAX PLANTS IN ANTHER CULTURE |
| title_short | DETERMINATION OF THE ORIGIN OF REGENERATED FLAX PLANTS IN ANTHER CULTURE |
| title_sort | determination of the origin of regenerated flax plants in anther culture |
| topic | ploidy anther culture doubled haploid segregation marker trait oil flax |
| url | https://vavilov.elpub.ru/jour/article/view/156 |
| work_keys_str_mv | AT aisoroka determinationoftheoriginofregeneratedflaxplantsinantherculture AT valyakh determinationoftheoriginofregeneratedflaxplantsinantherculture |