LncRNA HULC and miR-122 Expression Pattern in HCC-Related HCV Egyptian Patients
Hepatocellular carcinoma (HCC) is a highly prevalent malignancy. It is a common type of cancer in Egypt due to chronic virus C infection (HCV). Currently, the frequently used lab test is serum α-fetoprotein. However, its diagnostic value is challenging due to its low sensitivity and specificity. Gen...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-09-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/13/9/1669 |
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| author | Dalia A. Gaber Olfat Shaker Alaa Tarek Younis Mohamed El-Kassas |
| author_facet | Dalia A. Gaber Olfat Shaker Alaa Tarek Younis Mohamed El-Kassas |
| author_sort | Dalia A. Gaber |
| collection | DOAJ |
| description | Hepatocellular carcinoma (HCC) is a highly prevalent malignancy. It is a common type of cancer in Egypt due to chronic virus C infection (HCV). Currently, the frequently used lab test is serum α-fetoprotein. However, its diagnostic value is challenging due to its low sensitivity and specificity. Genetic biomarkers have recently provided new insights for cancer diagnostics. Herein, we quantified Lnc HULC and miR-122 gene expression to test their potential in diagnosis. Both biomarkers were tested in the sera of 60 HCC patients and 60 with chronic HCV using real-time RT-PCR. miR-122 was highly expressed in HCV patients with a significant difference from the HCC group (<i>p</i> = 0.004), which points towards its role in prognosis value as a predictor of HCC in patients with chronic HCV. HULC was more highly expressed in HCC patients than in the HCV group (<i>p</i> = 0.018), indicating its potential use in screening and the early diagnosis of HCC. The receiver operating characteristic (ROC) curve analysis showed their reliable sensitivity and specificity. Our results reveal that miR-122 can act as a prognostic tool for patients with chronic HCV. Furthermore, it is an early predictor of HCC. LncRNA HULC can be used as an early diagnostic tool for HCC. |
| first_indexed | 2024-03-09T23:55:48Z |
| format | Article |
| id | doaj.art-ba610e9378264f9884a249f7504707c2 |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-09T23:55:48Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-ba610e9378264f9884a249f7504707c22023-11-23T16:26:13ZengMDPI AGGenes2073-44252022-09-01139166910.3390/genes13091669LncRNA HULC and miR-122 Expression Pattern in HCC-Related HCV Egyptian PatientsDalia A. Gaber0Olfat Shaker1Alaa Tarek Younis2Mohamed El-Kassas3Medical Biochemistry & Molecular Biology Department, Faculty of Medicine, Helwan University, Cairo 11795, EgyptDepartment of Medical Biochemistry & Molecular Biology, Faculty of Medicine, Cairo University, Cairo 11956, EgyptMedical Biochemistry & Molecular Biology Department, Faculty of Medicine, Helwan University, Cairo 11795, EgyptEndemic Medicine Department, Faculty of Medicine, Helwan University, Cairo 11795, EgyptHepatocellular carcinoma (HCC) is a highly prevalent malignancy. It is a common type of cancer in Egypt due to chronic virus C infection (HCV). Currently, the frequently used lab test is serum α-fetoprotein. However, its diagnostic value is challenging due to its low sensitivity and specificity. Genetic biomarkers have recently provided new insights for cancer diagnostics. Herein, we quantified Lnc HULC and miR-122 gene expression to test their potential in diagnosis. Both biomarkers were tested in the sera of 60 HCC patients and 60 with chronic HCV using real-time RT-PCR. miR-122 was highly expressed in HCV patients with a significant difference from the HCC group (<i>p</i> = 0.004), which points towards its role in prognosis value as a predictor of HCC in patients with chronic HCV. HULC was more highly expressed in HCC patients than in the HCV group (<i>p</i> = 0.018), indicating its potential use in screening and the early diagnosis of HCC. The receiver operating characteristic (ROC) curve analysis showed their reliable sensitivity and specificity. Our results reveal that miR-122 can act as a prognostic tool for patients with chronic HCV. Furthermore, it is an early predictor of HCC. LncRNA HULC can be used as an early diagnostic tool for HCC.https://www.mdpi.com/2073-4425/13/9/1669miR-122HULCHCCHCVmolecular biomarkers |
| spellingShingle | Dalia A. Gaber Olfat Shaker Alaa Tarek Younis Mohamed El-Kassas LncRNA HULC and miR-122 Expression Pattern in HCC-Related HCV Egyptian Patients Genes miR-122 HULC HCC HCV molecular biomarkers |
| title | LncRNA HULC and miR-122 Expression Pattern in HCC-Related HCV Egyptian Patients |
| title_full | LncRNA HULC and miR-122 Expression Pattern in HCC-Related HCV Egyptian Patients |
| title_fullStr | LncRNA HULC and miR-122 Expression Pattern in HCC-Related HCV Egyptian Patients |
| title_full_unstemmed | LncRNA HULC and miR-122 Expression Pattern in HCC-Related HCV Egyptian Patients |
| title_short | LncRNA HULC and miR-122 Expression Pattern in HCC-Related HCV Egyptian Patients |
| title_sort | lncrna hulc and mir 122 expression pattern in hcc related hcv egyptian patients |
| topic | miR-122 HULC HCC HCV molecular biomarkers |
| url | https://www.mdpi.com/2073-4425/13/9/1669 |
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