Wheat beta-expansin (EXPB11) genes: Identification of the expressed gene on chromosome 3BS carrying a pollen allergen domain
<p>Abstract</p> <p>Background</p> <p>Expansins form a large multi-gene family found in wheat and other cereal genomes that are involved in the expansion of cell walls as a tissue grows. The expansin family can be divided up into two main groups, namely, alpha-expansin (...
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| Format: | Article |
| Language: | English |
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BMC
2010-05-01
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| Series: | BMC Plant Biology |
| Online Access: | http://www.biomedcentral.com/1471-2229/10/99 |
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| author | Jia Jizeng Zhang Juncheng Kong Xiuying Békés Ferenc Dunn David S Li Dora Breen James Wicker Thomas Mago Rohit Ma Wujun Bellgard Matthew Appels Rudi |
| author_facet | Jia Jizeng Zhang Juncheng Kong Xiuying Békés Ferenc Dunn David S Li Dora Breen James Wicker Thomas Mago Rohit Ma Wujun Bellgard Matthew Appels Rudi |
| author_sort | Jia Jizeng |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Expansins form a large multi-gene family found in wheat and other cereal genomes that are involved in the expansion of cell walls as a tissue grows. The expansin family can be divided up into two main groups, namely, alpha-expansin (EXPA) and beta-expansin proteins (EXPB), with the EXPB group being of particular interest as group 1-pollen allergens.</p> <p>Results</p> <p>In this study, three beta-expansin genes were identified and characterized from a newly sequenced region of the <it>Triticum aestivum </it>cv. Chinese Spring chromosome 3B physical map at the <it>Sr2 </it>locus (FPC contig <it>ctg11</it>). The analysis of a 357 kb sub-sequence of FPC contig <it>ctg11 </it>identified one beta-expansin genes to be <it>TaEXPB11</it>, originally identified as a cDNA from the wheat cv Wyuna. Through the analysis of intron sequences of the three wheat cv. Chinese Spring genes, we propose that two of these beta-expansin genes are duplications of the <it>TaEXPB11 </it>gene. Comparative sequence analysis with two other wheat cultivars (cv. Westonia and cv. Hope) and a <it>Triticum aestivum </it>var. <it>spelta </it>line validated the identification of the Chinese Spring variant of <it>TaEXPB11</it>. The expression in maternal and grain tissues was confirmed by examining EST databases and carrying out RT-PCR experiments. Detailed examination of the position of TaEXPB11 relative to the locus encoding <it>Sr2 </it>disease resistance ruled out the possibility of this gene directly contributing to the resistance phenotype.</p> <p>Conclusions</p> <p>Through 3-D structural protein comparisons with <it>Zea mays EXPB1</it>, we proposed that variations within the coding sequence of <it>TaEXPB11 </it>in wheats may produce a functional change within features such as domain 1 related to possible involvement in cell wall structure and domain 2 defining the pollen allergen domain and binding to IgE protein. The variation established in this gene suggests it is a clearly identifiable member of a gene family and reflects the dynamic features of the wheat genome as it adapted to a range of different environments and uses.</p> <p>Accession Numbers: <it>ctg11 </it>=FN564426</p> <p>Survey sequences of <it>TaEXPB11ws </it>and <it>TsEXPB11 </it>are provided request.</p> |
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| spelling | doaj.art-ba62d3bea68c43e0ac16a1dec331b92a2022-12-21T23:21:36ZengBMCBMC Plant Biology1471-22292010-05-011019910.1186/1471-2229-10-99Wheat beta-expansin (EXPB11) genes: Identification of the expressed gene on chromosome 3BS carrying a pollen allergen domainJia JizengZhang JunchengKong XiuyingBékés FerencDunn David SLi DoraBreen JamesWicker ThomasMago RohitMa WujunBellgard MatthewAppels Rudi<p>Abstract</p> <p>Background</p> <p>Expansins form a large multi-gene family found in wheat and other cereal genomes that are involved in the expansion of cell walls as a tissue grows. The expansin family can be divided up into two main groups, namely, alpha-expansin (EXPA) and beta-expansin proteins (EXPB), with the EXPB group being of particular interest as group 1-pollen allergens.</p> <p>Results</p> <p>In this study, three beta-expansin genes were identified and characterized from a newly sequenced region of the <it>Triticum aestivum </it>cv. Chinese Spring chromosome 3B physical map at the <it>Sr2 </it>locus (FPC contig <it>ctg11</it>). The analysis of a 357 kb sub-sequence of FPC contig <it>ctg11 </it>identified one beta-expansin genes to be <it>TaEXPB11</it>, originally identified as a cDNA from the wheat cv Wyuna. Through the analysis of intron sequences of the three wheat cv. Chinese Spring genes, we propose that two of these beta-expansin genes are duplications of the <it>TaEXPB11 </it>gene. Comparative sequence analysis with two other wheat cultivars (cv. Westonia and cv. Hope) and a <it>Triticum aestivum </it>var. <it>spelta </it>line validated the identification of the Chinese Spring variant of <it>TaEXPB11</it>. The expression in maternal and grain tissues was confirmed by examining EST databases and carrying out RT-PCR experiments. Detailed examination of the position of TaEXPB11 relative to the locus encoding <it>Sr2 </it>disease resistance ruled out the possibility of this gene directly contributing to the resistance phenotype.</p> <p>Conclusions</p> <p>Through 3-D structural protein comparisons with <it>Zea mays EXPB1</it>, we proposed that variations within the coding sequence of <it>TaEXPB11 </it>in wheats may produce a functional change within features such as domain 1 related to possible involvement in cell wall structure and domain 2 defining the pollen allergen domain and binding to IgE protein. The variation established in this gene suggests it is a clearly identifiable member of a gene family and reflects the dynamic features of the wheat genome as it adapted to a range of different environments and uses.</p> <p>Accession Numbers: <it>ctg11 </it>=FN564426</p> <p>Survey sequences of <it>TaEXPB11ws </it>and <it>TsEXPB11 </it>are provided request.</p>http://www.biomedcentral.com/1471-2229/10/99 |
| spellingShingle | Jia Jizeng Zhang Juncheng Kong Xiuying Békés Ferenc Dunn David S Li Dora Breen James Wicker Thomas Mago Rohit Ma Wujun Bellgard Matthew Appels Rudi Wheat beta-expansin (EXPB11) genes: Identification of the expressed gene on chromosome 3BS carrying a pollen allergen domain BMC Plant Biology |
| title | Wheat beta-expansin (EXPB11) genes: Identification of the expressed gene on chromosome 3BS carrying a pollen allergen domain |
| title_full | Wheat beta-expansin (EXPB11) genes: Identification of the expressed gene on chromosome 3BS carrying a pollen allergen domain |
| title_fullStr | Wheat beta-expansin (EXPB11) genes: Identification of the expressed gene on chromosome 3BS carrying a pollen allergen domain |
| title_full_unstemmed | Wheat beta-expansin (EXPB11) genes: Identification of the expressed gene on chromosome 3BS carrying a pollen allergen domain |
| title_short | Wheat beta-expansin (EXPB11) genes: Identification of the expressed gene on chromosome 3BS carrying a pollen allergen domain |
| title_sort | wheat beta expansin expb11 genes identification of the expressed gene on chromosome 3bs carrying a pollen allergen domain |
| url | http://www.biomedcentral.com/1471-2229/10/99 |
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