Molecular Characteristics of Batanghari, Tambago, Orange, and Mandiangin Giant Gourami Strains
<p class="IsiAbstrakIndo"><span lang="EN-GB">Morphological variations among geographic and can be identified as different species. However, a lot of studies proved that morphological variations are also common in conspecific individuals. Therefore, precise identificat...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Universitas Negeri Semarang
2017-12-01
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| Series: | Biosaintifika: Journal of Biology & Biology Education |
| Subjects: | |
| Online Access: | https://journal.unnes.ac.id/nju/index.php/biosaintifika/article/view/12097 |
| _version_ | 1818189725517742080 |
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| author | Agus Nuryanto Anatasia Endang Pulungsari |
| author_facet | Agus Nuryanto Anatasia Endang Pulungsari |
| author_sort | Agus Nuryanto |
| collection | DOAJ |
| description | <p class="IsiAbstrakIndo"><span lang="EN-GB">Morphological variations among geographic and can be identified as different species. However, a lot of studies proved that morphological variations are also common in conspecific individuals. Therefore, precise identification using additional characters is vital, such as using a molecular marker. Here, we characterized Batanghari, Tambago, Orange, and Mandiangin gourami strains using the cytochrome b gene to evaluate their taxonomic status. Partial sequences of cytochrome b gene were sequenced for 40 individuals. Taxonomic status was checked for giant gourami sequences available in GenBank. Kimura 2-Parameter genetic distances were calculated in MEGA6 software. Haplotype and nucleotide diversity within population and </span><span lang="EN-GB">Φ</span><sub><span lang="EN-GB">st</span></sub><span lang="EN-GB">-value among populations were estimated in Arlequin software. Phylogenetic relationship was reconstructed using the neighbor-joining method in MEGA6 software based on Kimura 2-parameter model with 1000 pseudobootstraps. Taxonomic identification results in 99% sequences homology to </span><em><span lang="EN-GB">Osphronemus goramy</span></em><span lang="EN-GB"> sequences (accession number KU984978.1 and AY763768.1), means that all strains belong to single species. Low genetic distances, medium haplotype and low-level nucleotide diversity were observed among strains. Pairwise </span><span lang="EN-GB">Φ</span><sub><span lang="EN-GB">st</span></sub><span lang="EN-GB">-comparison indicates no genetic differences among Sumatera strain, whereas strong genetic structures observed between Sumatera and Mandiangin strains. The phylogenetic tree showed that Mandiangin formed separate subclades from other strains with bootstraps value of 100%. This finding has important implication for breeding sciences and efforts.</span></p> |
| first_indexed | 2024-12-11T23:47:23Z |
| format | Article |
| id | doaj.art-ba66bcd1e6f246999152c3c8a7d8c287 |
| institution | Directory Open Access Journal |
| issn | 2085-191X 2338-7610 |
| language | English |
| last_indexed | 2024-12-11T23:47:23Z |
| publishDate | 2017-12-01 |
| publisher | Universitas Negeri Semarang |
| record_format | Article |
| series | Biosaintifika: Journal of Biology & Biology Education |
| spelling | doaj.art-ba66bcd1e6f246999152c3c8a7d8c2872022-12-22T00:45:35ZengUniversitas Negeri SemarangBiosaintifika: Journal of Biology & Biology Education2085-191X2338-76102017-12-019359259910.15294/biosaintifika.v9i3.120976826Molecular Characteristics of Batanghari, Tambago, Orange, and Mandiangin Giant Gourami StrainsAgus Nuryanto0Anatasia Endang Pulungsari1Faculty of Biology, Universitas Jenderal SoedirmanFaculty of Biology, Universitas Jenderal Soedirman<p class="IsiAbstrakIndo"><span lang="EN-GB">Morphological variations among geographic and can be identified as different species. However, a lot of studies proved that morphological variations are also common in conspecific individuals. Therefore, precise identification using additional characters is vital, such as using a molecular marker. Here, we characterized Batanghari, Tambago, Orange, and Mandiangin gourami strains using the cytochrome b gene to evaluate their taxonomic status. Partial sequences of cytochrome b gene were sequenced for 40 individuals. Taxonomic status was checked for giant gourami sequences available in GenBank. Kimura 2-Parameter genetic distances were calculated in MEGA6 software. Haplotype and nucleotide diversity within population and </span><span lang="EN-GB">Φ</span><sub><span lang="EN-GB">st</span></sub><span lang="EN-GB">-value among populations were estimated in Arlequin software. Phylogenetic relationship was reconstructed using the neighbor-joining method in MEGA6 software based on Kimura 2-parameter model with 1000 pseudobootstraps. Taxonomic identification results in 99% sequences homology to </span><em><span lang="EN-GB">Osphronemus goramy</span></em><span lang="EN-GB"> sequences (accession number KU984978.1 and AY763768.1), means that all strains belong to single species. Low genetic distances, medium haplotype and low-level nucleotide diversity were observed among strains. Pairwise </span><span lang="EN-GB">Φ</span><sub><span lang="EN-GB">st</span></sub><span lang="EN-GB">-comparison indicates no genetic differences among Sumatera strain, whereas strong genetic structures observed between Sumatera and Mandiangin strains. The phylogenetic tree showed that Mandiangin formed separate subclades from other strains with bootstraps value of 100%. This finding has important implication for breeding sciences and efforts.</span></p>https://journal.unnes.ac.id/nju/index.php/biosaintifika/article/view/12097genetic distancesgenetic diversityneighbor-joiningOsphronemus goramy |
| spellingShingle | Agus Nuryanto Anatasia Endang Pulungsari Molecular Characteristics of Batanghari, Tambago, Orange, and Mandiangin Giant Gourami Strains Biosaintifika: Journal of Biology & Biology Education genetic distances genetic diversity neighbor-joining Osphronemus goramy |
| title | Molecular Characteristics of Batanghari, Tambago, Orange, and Mandiangin Giant Gourami Strains |
| title_full | Molecular Characteristics of Batanghari, Tambago, Orange, and Mandiangin Giant Gourami Strains |
| title_fullStr | Molecular Characteristics of Batanghari, Tambago, Orange, and Mandiangin Giant Gourami Strains |
| title_full_unstemmed | Molecular Characteristics of Batanghari, Tambago, Orange, and Mandiangin Giant Gourami Strains |
| title_short | Molecular Characteristics of Batanghari, Tambago, Orange, and Mandiangin Giant Gourami Strains |
| title_sort | molecular characteristics of batanghari tambago orange and mandiangin giant gourami strains |
| topic | genetic distances genetic diversity neighbor-joining Osphronemus goramy |
| url | https://journal.unnes.ac.id/nju/index.php/biosaintifika/article/view/12097 |
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