Separation of polyhydroxyalkanoates-producing bacterial strains using PHA synthase gene and their evaluation for PHA deposition
In this study, a variety of samples were screened for the presence of PHA synthase gene. Results showed that 16 out of 102 isolated were positive for PHA respective genes. The highest prevalence was observed in Pseudomonas aeruginosa. The capability of PHA production was also shown by growing these...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Instituto de Tecnologia do Paraná (Tecpar)
2013-08-01
|
| Series: | Brazilian Archives of Biology and Technology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000400015&lng=en&tlng=en |
| _version_ | 1819207999322849280 |
|---|---|
| author | Abdul Basit Khan Mehmood Iqbal Khattak Omer Mukhtar Tarar Farzana Habib Khalid Jamil Arfa Yasmin Shoukat Parvez |
| author_facet | Abdul Basit Khan Mehmood Iqbal Khattak Omer Mukhtar Tarar Farzana Habib Khalid Jamil Arfa Yasmin Shoukat Parvez |
| author_sort | Abdul Basit Khan |
| collection | DOAJ |
| description | In this study, a variety of samples were screened for the presence of PHA synthase gene. Results showed that 16 out of 102 isolated were positive for PHA respective genes. The highest prevalence was observed in Pseudomonas aeruginosa. The capability of PHA production was also shown by growing these strains on the defined medium and subsequent analysis using intracellular granules staining and Fourier transform infrared spectroscopy (FT-IR). The microscopic analysis showed that the positive strains accumulated PHA in the cell. The FT-IR analysis showed the presence of PHA peaks in the dried cells as well as in extraction product. P aeruginosa strain P7 showed higher concentration of PHA compared to the others as demonstrated by the highest respective peaks in FT-IR. |
| first_indexed | 2024-12-23T05:32:25Z |
| format | Article |
| id | doaj.art-ba6a409be0f34262917de0ee1297608d |
| institution | Directory Open Access Journal |
| issn | 1678-4324 |
| language | English |
| last_indexed | 2024-12-23T05:32:25Z |
| publishDate | 2013-08-01 |
| publisher | Instituto de Tecnologia do Paraná (Tecpar) |
| record_format | Article |
| series | Brazilian Archives of Biology and Technology |
| spelling | doaj.art-ba6a409be0f34262917de0ee1297608d2022-12-21T17:58:26ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1678-43242013-08-0156464565210.1590/S1516-89132013000400015S1516-89132013000400015Separation of polyhydroxyalkanoates-producing bacterial strains using PHA synthase gene and their evaluation for PHA depositionAbdul Basit Khan0Mehmood Iqbal Khattak1Omer Mukhtar Tarar2Farzana Habib3Khalid Jamil4Arfa Yasmin5Shoukat Parvez6Food and Marine Resource Research CentreApplied Chemistry Research CentreFood and Marine Resource Research CentreApplied Chemistry Research CentreFood and Marine Resource Research CentreApplied Chemistry Research CentrePCSIR Laboratories ComplexIn this study, a variety of samples were screened for the presence of PHA synthase gene. Results showed that 16 out of 102 isolated were positive for PHA respective genes. The highest prevalence was observed in Pseudomonas aeruginosa. The capability of PHA production was also shown by growing these strains on the defined medium and subsequent analysis using intracellular granules staining and Fourier transform infrared spectroscopy (FT-IR). The microscopic analysis showed that the positive strains accumulated PHA in the cell. The FT-IR analysis showed the presence of PHA peaks in the dried cells as well as in extraction product. P aeruginosa strain P7 showed higher concentration of PHA compared to the others as demonstrated by the highest respective peaks in FT-IR.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000400015&lng=en&tlng=enPolyhydroxyalkanoatesBiodegradable polymersPHA synthase geneFTIR |
| spellingShingle | Abdul Basit Khan Mehmood Iqbal Khattak Omer Mukhtar Tarar Farzana Habib Khalid Jamil Arfa Yasmin Shoukat Parvez Separation of polyhydroxyalkanoates-producing bacterial strains using PHA synthase gene and their evaluation for PHA deposition Brazilian Archives of Biology and Technology Polyhydroxyalkanoates Biodegradable polymers PHA synthase gene FTIR |
| title | Separation of polyhydroxyalkanoates-producing bacterial strains using PHA synthase gene and their evaluation for PHA deposition |
| title_full | Separation of polyhydroxyalkanoates-producing bacterial strains using PHA synthase gene and their evaluation for PHA deposition |
| title_fullStr | Separation of polyhydroxyalkanoates-producing bacterial strains using PHA synthase gene and their evaluation for PHA deposition |
| title_full_unstemmed | Separation of polyhydroxyalkanoates-producing bacterial strains using PHA synthase gene and their evaluation for PHA deposition |
| title_short | Separation of polyhydroxyalkanoates-producing bacterial strains using PHA synthase gene and their evaluation for PHA deposition |
| title_sort | separation of polyhydroxyalkanoates producing bacterial strains using pha synthase gene and their evaluation for pha deposition |
| topic | Polyhydroxyalkanoates Biodegradable polymers PHA synthase gene FTIR |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000400015&lng=en&tlng=en |
| work_keys_str_mv | AT abdulbasitkhan separationofpolyhydroxyalkanoatesproducingbacterialstrainsusingphasynthasegeneandtheirevaluationforphadeposition AT mehmoodiqbalkhattak separationofpolyhydroxyalkanoatesproducingbacterialstrainsusingphasynthasegeneandtheirevaluationforphadeposition AT omermukhtartarar separationofpolyhydroxyalkanoatesproducingbacterialstrainsusingphasynthasegeneandtheirevaluationforphadeposition AT farzanahabib separationofpolyhydroxyalkanoatesproducingbacterialstrainsusingphasynthasegeneandtheirevaluationforphadeposition AT khalidjamil separationofpolyhydroxyalkanoatesproducingbacterialstrainsusingphasynthasegeneandtheirevaluationforphadeposition AT arfayasmin separationofpolyhydroxyalkanoatesproducingbacterialstrainsusingphasynthasegeneandtheirevaluationforphadeposition AT shoukatparvez separationofpolyhydroxyalkanoatesproducingbacterialstrainsusingphasynthasegeneandtheirevaluationforphadeposition |