Early diagnosis of bladder cancer by photoacoustic imaging of tumor-targeted gold nanorods
Detection and removal of bladder cancer lesions at an early stage is crucial for preventing tumor relapse and progression. This study aimed to develop a new technological platform for the visualization of small and flat urothelial lesions of high-grade bladder carcinoma in situ (CIS).We found that t...
| Main Authors: | , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-12-01
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| Series: | Photoacoustics |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213597922000659 |
| _version_ | 1828274720034783232 |
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| author | Elisa Alchera Matteo Monieri Mirko Maturi Irene Locatelli Erica Locatelli Silvia Tortorella Angelina Sacchi Angelo Corti Manuela Nebuloni Roberta Lucianò Filippo Pederzoli Francesco Montorsi Andrea Salonia Sandra Meyer Jithin Jose Pierangela Giustetto Mauro Comes Franchini Flavio Curnis Massimo Alfano |
| author_facet | Elisa Alchera Matteo Monieri Mirko Maturi Irene Locatelli Erica Locatelli Silvia Tortorella Angelina Sacchi Angelo Corti Manuela Nebuloni Roberta Lucianò Filippo Pederzoli Francesco Montorsi Andrea Salonia Sandra Meyer Jithin Jose Pierangela Giustetto Mauro Comes Franchini Flavio Curnis Massimo Alfano |
| author_sort | Elisa Alchera |
| collection | DOAJ |
| description | Detection and removal of bladder cancer lesions at an early stage is crucial for preventing tumor relapse and progression. This study aimed to develop a new technological platform for the visualization of small and flat urothelial lesions of high-grade bladder carcinoma in situ (CIS).We found that the integrin α5β1, overexpressed in bladder cancer cell lines, murine orthotopic bladder cancer and human bladder CIS, can be exploited as a receptor for targeted delivery of GNRs functionalized with the cyclic CphgisoDGRG peptide (Iso4). The GNRs@Chit-Iso4 was stable in urine and selectively recognized α5β1 positive neoplastic urothelium, while low frequency ultrasound-assisted shaking of intravesically instilled GNRs@Chit-Iso4 allowed the distribution of nanoparticles across the entire volume of the bladder. Photoacoustic imaging of GNRs@Chit-Iso4 bound to tumor cells allowed for the detection of neoplastic lesions smaller than 0.5 mm that were undetectable by ultrasound imaging and bioluminescence. |
| first_indexed | 2024-04-13T06:39:34Z |
| format | Article |
| id | doaj.art-ba6a54ba8e8040519884c8b617e052a3 |
| institution | Directory Open Access Journal |
| issn | 2213-5979 |
| language | English |
| last_indexed | 2024-04-13T06:39:34Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Photoacoustics |
| spelling | doaj.art-ba6a54ba8e8040519884c8b617e052a32022-12-22T02:57:47ZengElsevierPhotoacoustics2213-59792022-12-0128100400Early diagnosis of bladder cancer by photoacoustic imaging of tumor-targeted gold nanorodsElisa Alchera0Matteo Monieri1Mirko Maturi2Irene Locatelli3Erica Locatelli4Silvia Tortorella5Angelina Sacchi6Angelo Corti7Manuela Nebuloni8Roberta Lucianò9Filippo Pederzoli10Francesco Montorsi11Andrea Salonia12Sandra Meyer13Jithin Jose14Pierangela Giustetto15Mauro Comes Franchini16Flavio Curnis17Massimo Alfano18Unit of Urology, URI, Division of Experimental Oncology, IRCCS San Raffaele Scientific Institute, Milan, ItalyTumor Biology and Vascular Targeting Unit, Division of Experimental Oncology, IRCCS San Raffaele Scientific Institute, Milan, ItalyDepartment of Industrial Chemistry ''Toso Montanari'', University of Bologna, Bologna, ItalyUnit of Urology, URI, Division of Experimental Oncology, IRCCS San Raffaele Scientific Institute, Milan, ItalyDepartment of Industrial Chemistry ''Toso Montanari'', University of Bologna, Bologna, ItalyDepartment of Industrial Chemistry ''Toso Montanari'', University of Bologna, Bologna, ItalyTumor Biology and Vascular Targeting Unit, Division of Experimental Oncology, IRCCS San Raffaele Scientific Institute, Milan, ItalyTumor Biology and Vascular Targeting Unit, Division of Experimental Oncology, IRCCS San Raffaele Scientific Institute, Milan, Italy; Università Vita-Salute San Raffaele, Milan, ItalyPathology Unit, Department of Biomedical and Clinical Sciences, L. Sacco Hospital, Università degli Studi di Milano, Milan, ItalyDepartment of Pathology, IRCCS San Raffaele Hospital and Scientific Institute, Milan, ItalyUnit of Urology, URI, Division of Experimental Oncology, IRCCS San Raffaele Scientific Institute, Milan, Italy; Università Vita-Salute San Raffaele, Milan, ItalyUnit of Urology, URI, Division of Experimental Oncology, IRCCS San Raffaele Scientific Institute, Milan, Italy; Università Vita-Salute San Raffaele, Milan, ItalyUnit of Urology, URI, Division of Experimental Oncology, IRCCS San Raffaele Scientific Institute, Milan, Italy; Università Vita-Salute San Raffaele, Milan, ItalyFUJIFILM Visualsonics Inc., Amsterdam, the NetherlandsFUJIFILM Visualsonics Inc., Amsterdam, the NetherlandsFreedom Waves s.r.l., Milan, ItalyDepartment of Industrial Chemistry ''Toso Montanari'', University of Bologna, Bologna, Italy; Corresponding authors.Tumor Biology and Vascular Targeting Unit, Division of Experimental Oncology, IRCCS San Raffaele Scientific Institute, Milan, Italy; Corresponding authors.Unit of Urology, URI, Division of Experimental Oncology, IRCCS San Raffaele Scientific Institute, Milan, Italy; Corresponding authors.Detection and removal of bladder cancer lesions at an early stage is crucial for preventing tumor relapse and progression. This study aimed to develop a new technological platform for the visualization of small and flat urothelial lesions of high-grade bladder carcinoma in situ (CIS).We found that the integrin α5β1, overexpressed in bladder cancer cell lines, murine orthotopic bladder cancer and human bladder CIS, can be exploited as a receptor for targeted delivery of GNRs functionalized with the cyclic CphgisoDGRG peptide (Iso4). The GNRs@Chit-Iso4 was stable in urine and selectively recognized α5β1 positive neoplastic urothelium, while low frequency ultrasound-assisted shaking of intravesically instilled GNRs@Chit-Iso4 allowed the distribution of nanoparticles across the entire volume of the bladder. Photoacoustic imaging of GNRs@Chit-Iso4 bound to tumor cells allowed for the detection of neoplastic lesions smaller than 0.5 mm that were undetectable by ultrasound imaging and bioluminescence.http://www.sciencedirect.com/science/article/pii/S2213597922000659Tumor targetingBladder cancerGold nanorodsPhotoacoustic imagingEarly diagnosis |
| spellingShingle | Elisa Alchera Matteo Monieri Mirko Maturi Irene Locatelli Erica Locatelli Silvia Tortorella Angelina Sacchi Angelo Corti Manuela Nebuloni Roberta Lucianò Filippo Pederzoli Francesco Montorsi Andrea Salonia Sandra Meyer Jithin Jose Pierangela Giustetto Mauro Comes Franchini Flavio Curnis Massimo Alfano Early diagnosis of bladder cancer by photoacoustic imaging of tumor-targeted gold nanorods Photoacoustics Tumor targeting Bladder cancer Gold nanorods Photoacoustic imaging Early diagnosis |
| title | Early diagnosis of bladder cancer by photoacoustic imaging of tumor-targeted gold nanorods |
| title_full | Early diagnosis of bladder cancer by photoacoustic imaging of tumor-targeted gold nanorods |
| title_fullStr | Early diagnosis of bladder cancer by photoacoustic imaging of tumor-targeted gold nanorods |
| title_full_unstemmed | Early diagnosis of bladder cancer by photoacoustic imaging of tumor-targeted gold nanorods |
| title_short | Early diagnosis of bladder cancer by photoacoustic imaging of tumor-targeted gold nanorods |
| title_sort | early diagnosis of bladder cancer by photoacoustic imaging of tumor targeted gold nanorods |
| topic | Tumor targeting Bladder cancer Gold nanorods Photoacoustic imaging Early diagnosis |
| url | http://www.sciencedirect.com/science/article/pii/S2213597922000659 |
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