Core Fucosylation of the T Cell Receptor Is Required for T Cell Activation
CD4+ T cell activation promotes the pathogenic process of systemic lupus erythematosus (SLE). T cell receptor (TCR) complex are highly core fucosylated glycoproteins, which play important roles in T cell activation. In this study, we found that the core fucosylation of CD4+ T cells was significantly...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-01-01
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| Series: | Frontiers in Immunology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fimmu.2018.00078/full |
| _version_ | 1811306826662150144 |
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| author | Wei Liang Shanshan Mao Shijie Sun Ming Li Zhi Li Rui Yu Tonghui Ma Jianguo Gu Jianing Zhang Naoyuki Taniguchi Wenzhe Li |
| author_facet | Wei Liang Shanshan Mao Shijie Sun Ming Li Zhi Li Rui Yu Tonghui Ma Jianguo Gu Jianing Zhang Naoyuki Taniguchi Wenzhe Li |
| author_sort | Wei Liang |
| collection | DOAJ |
| description | CD4+ T cell activation promotes the pathogenic process of systemic lupus erythematosus (SLE). T cell receptor (TCR) complex are highly core fucosylated glycoproteins, which play important roles in T cell activation. In this study, we found that the core fucosylation of CD4+ T cells was significantly increased in SLE patients. Loss of core fucosyltransferase (Fut8), the sole enzyme for catalyzing the core fucosylation of N-glycan, significantly reduced CD4+ T cell activation and ameliorated the experimental autoimmune encephalomyelitis-induced syndrome in Fut8−/− mice. T cell activation with OVA323–339 loaded major histocompatibility complex II (pMHC-II) on B cell was dramatically attenuated in Fut8−/−OT-II CD4+ T cells compared with Fut8+/+OT-II CD4+ T cells. Moreover, the phosphorylation of ZAP-70 was significantly reduced in Fut8+/+OT-II CD4+ T cells by the treatment of fucosidase. Our results suggest that core fucosylation is required for efficient TCR–pMHC-II contacts in CD4+ T cell activation, and hyper core fucosylation may serve as a potential novel biomarker in the sera from SLE patients. |
| first_indexed | 2024-04-13T08:51:43Z |
| format | Article |
| id | doaj.art-ba6a71024e8549cead2b785fc131b782 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-04-13T08:51:43Z |
| publishDate | 2018-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-ba6a71024e8549cead2b785fc131b7822022-12-22T02:53:27ZengFrontiers Media S.A.Frontiers in Immunology1664-32242018-01-01910.3389/fimmu.2018.00078315470Core Fucosylation of the T Cell Receptor Is Required for T Cell ActivationWei Liang0Shanshan Mao1Shijie Sun2Ming Li3Zhi Li4Rui Yu5Tonghui Ma6Jianguo Gu7Jianing Zhang8Naoyuki Taniguchi9Wenzhe Li10College of Basic Medical Sciences, Dalian Medical University, Dalian, ChinaCollege of Basic Medical Sciences, Dalian Medical University, Dalian, ChinaCollege of Basic Medical Sciences, Dalian Medical University, Dalian, ChinaCollege of Basic Medical Sciences, Dalian Medical University, Dalian, ChinaClinical Laboratory, Dalian Municipal Central Hospital, Dalian, ChinaCollege of Basic Medical Sciences, Dalian Medical University, Dalian, ChinaCollege of Basic Medical Sciences, Dalian Medical University, Dalian, ChinaDivision of Regulatory Glycobiology, Institute of Molecular Biomembrane and Glycobiology, Tohoku Medical and Pharmaceutical University, Sendai, JapanSchool of Life Science and Medicine, Dalian University of Technology, Panjin, ChinaSystems Glycobiology Research Group, Advanced Science Institute, RIKEN, Saitama, JapanCollege of Basic Medical Sciences, Dalian Medical University, Dalian, ChinaCD4+ T cell activation promotes the pathogenic process of systemic lupus erythematosus (SLE). T cell receptor (TCR) complex are highly core fucosylated glycoproteins, which play important roles in T cell activation. In this study, we found that the core fucosylation of CD4+ T cells was significantly increased in SLE patients. Loss of core fucosyltransferase (Fut8), the sole enzyme for catalyzing the core fucosylation of N-glycan, significantly reduced CD4+ T cell activation and ameliorated the experimental autoimmune encephalomyelitis-induced syndrome in Fut8−/− mice. T cell activation with OVA323–339 loaded major histocompatibility complex II (pMHC-II) on B cell was dramatically attenuated in Fut8−/−OT-II CD4+ T cells compared with Fut8+/+OT-II CD4+ T cells. Moreover, the phosphorylation of ZAP-70 was significantly reduced in Fut8+/+OT-II CD4+ T cells by the treatment of fucosidase. Our results suggest that core fucosylation is required for efficient TCR–pMHC-II contacts in CD4+ T cell activation, and hyper core fucosylation may serve as a potential novel biomarker in the sera from SLE patients.http://journal.frontiersin.org/article/10.3389/fimmu.2018.00078/fullcore fucosylationT cell receptorT cell activationsystemic lupus erythematosusT–B cell interaction |
| spellingShingle | Wei Liang Shanshan Mao Shijie Sun Ming Li Zhi Li Rui Yu Tonghui Ma Jianguo Gu Jianing Zhang Naoyuki Taniguchi Wenzhe Li Core Fucosylation of the T Cell Receptor Is Required for T Cell Activation Frontiers in Immunology core fucosylation T cell receptor T cell activation systemic lupus erythematosus T–B cell interaction |
| title | Core Fucosylation of the T Cell Receptor Is Required for T Cell Activation |
| title_full | Core Fucosylation of the T Cell Receptor Is Required for T Cell Activation |
| title_fullStr | Core Fucosylation of the T Cell Receptor Is Required for T Cell Activation |
| title_full_unstemmed | Core Fucosylation of the T Cell Receptor Is Required for T Cell Activation |
| title_short | Core Fucosylation of the T Cell Receptor Is Required for T Cell Activation |
| title_sort | core fucosylation of the t cell receptor is required for t cell activation |
| topic | core fucosylation T cell receptor T cell activation systemic lupus erythematosus T–B cell interaction |
| url | http://journal.frontiersin.org/article/10.3389/fimmu.2018.00078/full |
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