Effect of anaerobic phases length on denitrifying dephosphatation biocenosis – a case study of IFAS-MBSBBR
Abstract Background The study aimed to evaluate the influence of the duration times of anaerobic phases on the bacterial biocenosis characterisation while denitrifying dephosphatation in the Integrated Fixed-Film Activated Sludge – Moving-Bed Sequencing Batch Biofilm Reactor (IFAS-MBSBBR). The exper...
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| Format: | Article |
| Language: | English |
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BMC
2020-07-01
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| Series: | BMC Microbiology |
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| Online Access: | http://link.springer.com/article/10.1186/s12866-020-01896-3 |
| _version_ | 1818516598420406272 |
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| author | Anna Gnida Monika Żubrowska-Sudoł Katarzyna Sytek-Szmeichel Jolanta Podedworna Joanna Surmacz-Górska Dorota Marciocha |
| author_facet | Anna Gnida Monika Żubrowska-Sudoł Katarzyna Sytek-Szmeichel Jolanta Podedworna Joanna Surmacz-Górska Dorota Marciocha |
| author_sort | Anna Gnida |
| collection | DOAJ |
| description | Abstract Background The study aimed to evaluate the influence of the duration times of anaerobic phases on the bacterial biocenosis characterisation while denitrifying dephosphatation in the Integrated Fixed-Film Activated Sludge – Moving-Bed Sequencing Batch Biofilm Reactor (IFAS-MBSBBR). The experiment was conducted in a laboratory model. The study consisted of four series, which differed in terms of the ratio of the anaerobic phases. duration concerning the overall reaction time in the cycle. The anaerobic phases covered from 18 to 30% of the whole cycle duration. During the reactor performance that took 9 months, the influent and effluent were monitored by analysis of COD, TKN, NH4-N, NO2-N, NO3-N, TP, PO4-P, pH, alkalinity and the phosphorus uptake batch tests. Characterisation of the activated sludge and the biofilm biocenosis was based on fluorescent in situ hybridisation (identification of PAO and GAO) and the denaturing gradient gel electrophoresis patterns. Results The organic compounds removal was high (more than 95.7%) independently of cycle configuration. The best efficiency for nitrogen (91.1%) and phosphorus (98.8%) removal was achieved for the 30% share of the anaerobic phases in the reaction time. Denitrifying PAO (DPAO) covered more than 90% of PAO in the biofilm and usually around 70% of PAO in the activated sludge. A substantial part of the polyphosphate accumulating organisms (PAO) community were Actinobacteria. The denitrifying dephosphatation activity was performed mainly by Accumulibacter phosphatis. Conclusions High nutrient removal efficiencies may be obtained in IFAS-MBSBBR using the denitrifying dephosphatation process. It was found that the length of anaerobic phases influenced denitrification and the biological phosphorus removal. The extension of the anaerobic phases duration time in the reaction time caused an increase in the percentage share of denitrifying PAO (DPAO) in PAO. The biocenosis of the biofilm and the activated sludge reveal different species patterns and domination of the EBPR community. |
| first_indexed | 2024-12-11T00:44:27Z |
| format | Article |
| id | doaj.art-ba6abefb21404798bd9c20df8eedf665 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-12-11T00:44:27Z |
| publishDate | 2020-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-ba6abefb21404798bd9c20df8eedf6652022-12-22T01:26:49ZengBMCBMC Microbiology1471-21802020-07-0120111210.1186/s12866-020-01896-3Effect of anaerobic phases length on denitrifying dephosphatation biocenosis – a case study of IFAS-MBSBBRAnna Gnida0Monika Żubrowska-Sudoł1Katarzyna Sytek-Szmeichel2Jolanta Podedworna3Joanna Surmacz-Górska4Dorota Marciocha5Department of Environmental Biotechnology, Faculty of Energy and Environmental Engineering, Silesian University of TechnologyFaculty of Building Services, Hydro and Environmental Engineering, Warsaw University of TechnologyFaculty of Building Services, Hydro and Environmental Engineering, Warsaw University of TechnologyFaculty of Building Services, Hydro and Environmental Engineering, Warsaw University of TechnologyDepartment of Environmental Biotechnology, Faculty of Energy and Environmental Engineering, Silesian University of TechnologyDepartment of Environmental Biotechnology, Faculty of Energy and Environmental Engineering, Silesian University of TechnologyAbstract Background The study aimed to evaluate the influence of the duration times of anaerobic phases on the bacterial biocenosis characterisation while denitrifying dephosphatation in the Integrated Fixed-Film Activated Sludge – Moving-Bed Sequencing Batch Biofilm Reactor (IFAS-MBSBBR). The experiment was conducted in a laboratory model. The study consisted of four series, which differed in terms of the ratio of the anaerobic phases. duration concerning the overall reaction time in the cycle. The anaerobic phases covered from 18 to 30% of the whole cycle duration. During the reactor performance that took 9 months, the influent and effluent were monitored by analysis of COD, TKN, NH4-N, NO2-N, NO3-N, TP, PO4-P, pH, alkalinity and the phosphorus uptake batch tests. Characterisation of the activated sludge and the biofilm biocenosis was based on fluorescent in situ hybridisation (identification of PAO and GAO) and the denaturing gradient gel electrophoresis patterns. Results The organic compounds removal was high (more than 95.7%) independently of cycle configuration. The best efficiency for nitrogen (91.1%) and phosphorus (98.8%) removal was achieved for the 30% share of the anaerobic phases in the reaction time. Denitrifying PAO (DPAO) covered more than 90% of PAO in the biofilm and usually around 70% of PAO in the activated sludge. A substantial part of the polyphosphate accumulating organisms (PAO) community were Actinobacteria. The denitrifying dephosphatation activity was performed mainly by Accumulibacter phosphatis. Conclusions High nutrient removal efficiencies may be obtained in IFAS-MBSBBR using the denitrifying dephosphatation process. It was found that the length of anaerobic phases influenced denitrification and the biological phosphorus removal. The extension of the anaerobic phases duration time in the reaction time caused an increase in the percentage share of denitrifying PAO (DPAO) in PAO. The biocenosis of the biofilm and the activated sludge reveal different species patterns and domination of the EBPR community.http://link.springer.com/article/10.1186/s12866-020-01896-3Denitrifying dephosphatationPolyphosphate accumulating organismsMoving bed reactorActivated sludgeBiofilmWastewater treatment |
| spellingShingle | Anna Gnida Monika Żubrowska-Sudoł Katarzyna Sytek-Szmeichel Jolanta Podedworna Joanna Surmacz-Górska Dorota Marciocha Effect of anaerobic phases length on denitrifying dephosphatation biocenosis – a case study of IFAS-MBSBBR BMC Microbiology Denitrifying dephosphatation Polyphosphate accumulating organisms Moving bed reactor Activated sludge Biofilm Wastewater treatment |
| title | Effect of anaerobic phases length on denitrifying dephosphatation biocenosis – a case study of IFAS-MBSBBR |
| title_full | Effect of anaerobic phases length on denitrifying dephosphatation biocenosis – a case study of IFAS-MBSBBR |
| title_fullStr | Effect of anaerobic phases length on denitrifying dephosphatation biocenosis – a case study of IFAS-MBSBBR |
| title_full_unstemmed | Effect of anaerobic phases length on denitrifying dephosphatation biocenosis – a case study of IFAS-MBSBBR |
| title_short | Effect of anaerobic phases length on denitrifying dephosphatation biocenosis – a case study of IFAS-MBSBBR |
| title_sort | effect of anaerobic phases length on denitrifying dephosphatation biocenosis a case study of ifas mbsbbr |
| topic | Denitrifying dephosphatation Polyphosphate accumulating organisms Moving bed reactor Activated sludge Biofilm Wastewater treatment |
| url | http://link.springer.com/article/10.1186/s12866-020-01896-3 |
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