Laser Capture Microdissection: A Gear for Pancreatic Cancer Research
The advancement in molecular techniques has been attributed to the quality and significance of cancer research. Pancreatic cancer (PC) is one of the rare cancers with aggressive behavior and a high mortality rate. The asymptomatic nature of the disease until its advanced stage has resulted in late d...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/23/14566 |
| _version_ | 1797463164968763392 |
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| author | Bhavana Hemantha Rao Pavel Souček Viktor Hlaváč |
| author_facet | Bhavana Hemantha Rao Pavel Souček Viktor Hlaváč |
| author_sort | Bhavana Hemantha Rao |
| collection | DOAJ |
| description | The advancement in molecular techniques has been attributed to the quality and significance of cancer research. Pancreatic cancer (PC) is one of the rare cancers with aggressive behavior and a high mortality rate. The asymptomatic nature of the disease until its advanced stage has resulted in late diagnosis as well as poor prognosis. The heterogeneous character of PC has complicated cancer development and progression studies. The analysis of bulk tissues of the disease was insufficient to understand the disease, hence, the introduction of the single-cell separating technique aided researchers to decipher more about the specific cell population of tumors. This review gives an overview of the Laser Capture Microdissection (LCM) technique, one of the single-cell separation methods used in PC research. |
| first_indexed | 2024-03-09T17:47:46Z |
| format | Article |
| id | doaj.art-ba6efb0cdec04562936bd19331d17649 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T17:47:46Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-ba6efb0cdec04562936bd19331d176492023-11-24T11:04:08ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-11-0123231456610.3390/ijms232314566Laser Capture Microdissection: A Gear for Pancreatic Cancer ResearchBhavana Hemantha Rao0Pavel Souček1Viktor Hlaváč2Biomedical Center, Faculty of Medicine in Pilsen, Charles University, 306 05 Pilsen, Czech RepublicBiomedical Center, Faculty of Medicine in Pilsen, Charles University, 306 05 Pilsen, Czech RepublicBiomedical Center, Faculty of Medicine in Pilsen, Charles University, 306 05 Pilsen, Czech RepublicThe advancement in molecular techniques has been attributed to the quality and significance of cancer research. Pancreatic cancer (PC) is one of the rare cancers with aggressive behavior and a high mortality rate. The asymptomatic nature of the disease until its advanced stage has resulted in late diagnosis as well as poor prognosis. The heterogeneous character of PC has complicated cancer development and progression studies. The analysis of bulk tissues of the disease was insufficient to understand the disease, hence, the introduction of the single-cell separating technique aided researchers to decipher more about the specific cell population of tumors. This review gives an overview of the Laser Capture Microdissection (LCM) technique, one of the single-cell separation methods used in PC research.https://www.mdpi.com/1422-0067/23/23/14566Laser Capture Microdissection (LCM)pancreatic cancerintraductal papillary mucinous neoplasm (IPMN)single-cell separationhypoxiametastasis |
| spellingShingle | Bhavana Hemantha Rao Pavel Souček Viktor Hlaváč Laser Capture Microdissection: A Gear for Pancreatic Cancer Research International Journal of Molecular Sciences Laser Capture Microdissection (LCM) pancreatic cancer intraductal papillary mucinous neoplasm (IPMN) single-cell separation hypoxia metastasis |
| title | Laser Capture Microdissection: A Gear for Pancreatic Cancer Research |
| title_full | Laser Capture Microdissection: A Gear for Pancreatic Cancer Research |
| title_fullStr | Laser Capture Microdissection: A Gear for Pancreatic Cancer Research |
| title_full_unstemmed | Laser Capture Microdissection: A Gear for Pancreatic Cancer Research |
| title_short | Laser Capture Microdissection: A Gear for Pancreatic Cancer Research |
| title_sort | laser capture microdissection a gear for pancreatic cancer research |
| topic | Laser Capture Microdissection (LCM) pancreatic cancer intraductal papillary mucinous neoplasm (IPMN) single-cell separation hypoxia metastasis |
| url | https://www.mdpi.com/1422-0067/23/23/14566 |
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