Antimicrobial Efficacy of Glass Ionomer Cement in Incorporation with Biogenic <i>Zingiber officinale</i> Capped Silver-Nanobiotic, Chlorhexidine Diacetate and Lyophilized Miswak
In the present study, <i>Zingiber officinale</i> is used for the synthesis of <i>Zingiber officinale</i> capped silver nanoparticles (ZOE-AgNPs) and compares the antimicrobial efficacy and compressive strength of conventional glass ionomer cement (GIC) combined with ZOE-AgNPs...
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MDPI AG
2022-01-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/2/528 |
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| author | Amal Adnan Ashour Sakeenabi Basha Nayef H. Felemban Enas T. Enan Amal Ahmed Alyamani Sanaa M. F. Gad El-Rab |
| author_facet | Amal Adnan Ashour Sakeenabi Basha Nayef H. Felemban Enas T. Enan Amal Ahmed Alyamani Sanaa M. F. Gad El-Rab |
| author_sort | Amal Adnan Ashour |
| collection | DOAJ |
| description | In the present study, <i>Zingiber officinale</i> is used for the synthesis of <i>Zingiber officinale</i> capped silver nanoparticles (ZOE-AgNPs) and compares the antimicrobial efficacy and compressive strength of conventional glass ionomer cement (GIC) combined with ZOE-AgNPs, lyophilized miswak, and chlorhexidine diacetate (CHX) against oral microbes. Five groups of the disc-shaped GIC specimens were prepared. Group A: lyophilized miswak and GIC combination, Group B: ZOE-AgNPs and GIC combinations, Group C: CHX and GIC combination, Group D: ZOE-AgNPs + CHX + GIC; Group E: Conventional GIC. Results confirmed the successful formation of ZOE-AgNPs that was monitored by UV-Vis sharp absorption spectra at 415 nm. The X-ray diffractometer (XRD) and transmission electron microscope (TEM) results revealed the formation of ZOE-AgNPs with a mean size 10.5–14.12 nm. The peaks of the Fourier transform infrared spectroscopy (FTIR) were appearing the involvement of ZOE components onto the surface of ZOE-AgNPs which played as bioreducing, and stabilizing agents. At a 24-h, one-week and three-week intervals, Group D showed the significantly highest mean inhibitory zones compared to Group A, Group B, and Group C. At microbe-level comparison, <i>Streptococcus mutans</i> and <i>Staphylococcus aureus</i> were inhibited significantly by all the specimens tested except group E when compared to <i>Candida albicans</i>. Group D specimens showed slightly higher (45.8 ± 5.4) mean compressive strength in comparison with other groups. The combination of GIC with ZOE-AgNPs and chlorhexidine together enhanced its antimicrobial efficacy and compressive strength compared to GIC with ZOE-AgNPs or lyophilized miswak or chlorhexidine combination alone. The present study revealed that The combination of GIC with active components of ZOE-AgNPs and chlorhexidine paves the way to lead its effective nano-dental materials applications. |
| first_indexed | 2024-03-10T00:50:16Z |
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| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T00:50:16Z |
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| spelling | doaj.art-ba7072970c8d440a90712eb710613cec2023-11-23T14:53:09ZengMDPI AGMolecules1420-30492022-01-0127252810.3390/molecules27020528Antimicrobial Efficacy of Glass Ionomer Cement in Incorporation with Biogenic <i>Zingiber officinale</i> Capped Silver-Nanobiotic, Chlorhexidine Diacetate and Lyophilized MiswakAmal Adnan Ashour0Sakeenabi Basha1Nayef H. Felemban2Enas T. Enan3Amal Ahmed Alyamani4Sanaa M. F. Gad El-Rab5Department of Oral and Maxillofacial Surgery and Diagnostic Sciences, Oral Pathology Division, Faculty of Dentistry, Taif University, Taif 26571, Saudi ArabiaDepartment of Preventive and Community Dentistry, Faculty of Dentistry, Taif University, Taif 26571, Saudi ArabiaPreventive Dentistry Department, Faculty of Dentistry, Taif University, Taif 26571, Saudi ArabiaDepartment of Dental Biomaterials, Faculty of Dentistry, Mansoura University, Mansoura 35511, EgyptDepartment of Biotechnology, Faculty of Science, Taif University, Taif 21974, Saudi ArabiaDepartment of Botany and Microbiology, Faculty of Science, Assiut University, Assiut 71516, EgyptIn the present study, <i>Zingiber officinale</i> is used for the synthesis of <i>Zingiber officinale</i> capped silver nanoparticles (ZOE-AgNPs) and compares the antimicrobial efficacy and compressive strength of conventional glass ionomer cement (GIC) combined with ZOE-AgNPs, lyophilized miswak, and chlorhexidine diacetate (CHX) against oral microbes. Five groups of the disc-shaped GIC specimens were prepared. Group A: lyophilized miswak and GIC combination, Group B: ZOE-AgNPs and GIC combinations, Group C: CHX and GIC combination, Group D: ZOE-AgNPs + CHX + GIC; Group E: Conventional GIC. Results confirmed the successful formation of ZOE-AgNPs that was monitored by UV-Vis sharp absorption spectra at 415 nm. The X-ray diffractometer (XRD) and transmission electron microscope (TEM) results revealed the formation of ZOE-AgNPs with a mean size 10.5–14.12 nm. The peaks of the Fourier transform infrared spectroscopy (FTIR) were appearing the involvement of ZOE components onto the surface of ZOE-AgNPs which played as bioreducing, and stabilizing agents. At a 24-h, one-week and three-week intervals, Group D showed the significantly highest mean inhibitory zones compared to Group A, Group B, and Group C. At microbe-level comparison, <i>Streptococcus mutans</i> and <i>Staphylococcus aureus</i> were inhibited significantly by all the specimens tested except group E when compared to <i>Candida albicans</i>. Group D specimens showed slightly higher (45.8 ± 5.4) mean compressive strength in comparison with other groups. The combination of GIC with ZOE-AgNPs and chlorhexidine together enhanced its antimicrobial efficacy and compressive strength compared to GIC with ZOE-AgNPs or lyophilized miswak or chlorhexidine combination alone. The present study revealed that The combination of GIC with active components of ZOE-AgNPs and chlorhexidine paves the way to lead its effective nano-dental materials applications.https://www.mdpi.com/1420-3049/27/2/528GICZOE-AgNPsmiswakchlorhexidineantimicrobial efficacy |
| spellingShingle | Amal Adnan Ashour Sakeenabi Basha Nayef H. Felemban Enas T. Enan Amal Ahmed Alyamani Sanaa M. F. Gad El-Rab Antimicrobial Efficacy of Glass Ionomer Cement in Incorporation with Biogenic <i>Zingiber officinale</i> Capped Silver-Nanobiotic, Chlorhexidine Diacetate and Lyophilized Miswak Molecules GIC ZOE-AgNPs miswak chlorhexidine antimicrobial efficacy |
| title | Antimicrobial Efficacy of Glass Ionomer Cement in Incorporation with Biogenic <i>Zingiber officinale</i> Capped Silver-Nanobiotic, Chlorhexidine Diacetate and Lyophilized Miswak |
| title_full | Antimicrobial Efficacy of Glass Ionomer Cement in Incorporation with Biogenic <i>Zingiber officinale</i> Capped Silver-Nanobiotic, Chlorhexidine Diacetate and Lyophilized Miswak |
| title_fullStr | Antimicrobial Efficacy of Glass Ionomer Cement in Incorporation with Biogenic <i>Zingiber officinale</i> Capped Silver-Nanobiotic, Chlorhexidine Diacetate and Lyophilized Miswak |
| title_full_unstemmed | Antimicrobial Efficacy of Glass Ionomer Cement in Incorporation with Biogenic <i>Zingiber officinale</i> Capped Silver-Nanobiotic, Chlorhexidine Diacetate and Lyophilized Miswak |
| title_short | Antimicrobial Efficacy of Glass Ionomer Cement in Incorporation with Biogenic <i>Zingiber officinale</i> Capped Silver-Nanobiotic, Chlorhexidine Diacetate and Lyophilized Miswak |
| title_sort | antimicrobial efficacy of glass ionomer cement in incorporation with biogenic i zingiber officinale i capped silver nanobiotic chlorhexidine diacetate and lyophilized miswak |
| topic | GIC ZOE-AgNPs miswak chlorhexidine antimicrobial efficacy |
| url | https://www.mdpi.com/1420-3049/27/2/528 |
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