Extraction Optimization and Structural Characteristics of Chitosan from Cuttlefish (<i>S. pharaonis</i> sp.) Bone
In fish processing, reducing the waste rate and increasing the economic value of products is an important issue for global environmental protection and resource sustainability. It has been discovered that cuttlefish bones can be an excellent resource for producing attractive amounts of chitin and ch...
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2022-11-01
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| author | Sulfath Hakkim Hazeena Chih-Yao Hou Jing-Huei Zeng Bo-Heng Li Tzu-Chih Lin Cai-Sian Liu Chi-I Chang Shu-Ling Hsieh Ming-Kuei Shih |
| author_facet | Sulfath Hakkim Hazeena Chih-Yao Hou Jing-Huei Zeng Bo-Heng Li Tzu-Chih Lin Cai-Sian Liu Chi-I Chang Shu-Ling Hsieh Ming-Kuei Shih |
| author_sort | Sulfath Hakkim Hazeena |
| collection | DOAJ |
| description | In fish processing, reducing the waste rate and increasing the economic value of products is an important issue for global environmental protection and resource sustainability. It has been discovered that cuttlefish bones can be an excellent resource for producing attractive amounts of chitin and chitosan. Therefore, this study optimized chitosan extraction conditions using response surface methodology (RSM) to establish application conditions suitable for industrial production and reducing environmental impact. In addition, Fourier-transform infrared spectroscopy (FTIR), <sup>1</sup>H NMR and scanning electron microscope (SEM) characteristics of extracted chitosan were evaluated. The optimum extraction conditions for chitosan from cuttlebone chitin were 12.5M NaOH, 6 h and 80 °C, and the highest average yield was 56.47%. FTIR spectroscopy, <sup>1</sup>H NMR, and SEM identification proved that the chitosan prepared from cuttlefish bone has a unique molecular structure, and the degree of deacetylation of chitosan was about 81.3%. In addition, it was also confirmed that chitosan has significant anti-oxidation and oil-absorbing abilities. This research has successfully transformed the by-products of cuttlefish processing into value-added products. The process not only achieved the recycling and utilization of by-products but also enhanced industrial competitiveness and resource sustainability. |
| first_indexed | 2024-03-09T18:13:05Z |
| format | Article |
| id | doaj.art-ba71a386a9f0469ca0799ed609496208 |
| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-09T18:13:05Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Materials |
| spelling | doaj.art-ba71a386a9f0469ca0799ed6094962082023-11-24T09:02:00ZengMDPI AGMaterials1996-19442022-11-011522796910.3390/ma15227969Extraction Optimization and Structural Characteristics of Chitosan from Cuttlefish (<i>S. pharaonis</i> sp.) BoneSulfath Hakkim Hazeena0Chih-Yao Hou1Jing-Huei Zeng2Bo-Heng Li3Tzu-Chih Lin4Cai-Sian Liu5Chi-I Chang6Shu-Ling Hsieh7Ming-Kuei Shih8Department of Seafood Science, College of Hydrosphere, National Kaohsiung University of Science and Technology, Kaohsiung 81157, TaiwanDepartment of Seafood Science, College of Hydrosphere, National Kaohsiung University of Science and Technology, Kaohsiung 81157, TaiwanDepartment of Seafood Science, College of Hydrosphere, National Kaohsiung University of Science and Technology, Kaohsiung 81157, TaiwanDepartment of Seafood Science, College of Hydrosphere, National Kaohsiung University of Science and Technology, Kaohsiung 81157, TaiwanHong Yu Foods Company, Limited, Kaohsiung 806042, TaiwanDepartment of Seafood Science, College of Hydrosphere, National Kaohsiung University of Science and Technology, Kaohsiung 81157, TaiwanDepartment of Biological Science and Technology, National Pingtung University of Science and Technology, Pingtung 91201, TaiwanDepartment of Seafood Science, College of Hydrosphere, National Kaohsiung University of Science and Technology, Kaohsiung 81157, TaiwanGraduate Institute of Food Culture and Innovation, National Kaohsiung University of Hospitality and Tourism, Kaohsiung 812301, TaiwanIn fish processing, reducing the waste rate and increasing the economic value of products is an important issue for global environmental protection and resource sustainability. It has been discovered that cuttlefish bones can be an excellent resource for producing attractive amounts of chitin and chitosan. Therefore, this study optimized chitosan extraction conditions using response surface methodology (RSM) to establish application conditions suitable for industrial production and reducing environmental impact. In addition, Fourier-transform infrared spectroscopy (FTIR), <sup>1</sup>H NMR and scanning electron microscope (SEM) characteristics of extracted chitosan were evaluated. The optimum extraction conditions for chitosan from cuttlebone chitin were 12.5M NaOH, 6 h and 80 °C, and the highest average yield was 56.47%. FTIR spectroscopy, <sup>1</sup>H NMR, and SEM identification proved that the chitosan prepared from cuttlefish bone has a unique molecular structure, and the degree of deacetylation of chitosan was about 81.3%. In addition, it was also confirmed that chitosan has significant anti-oxidation and oil-absorbing abilities. This research has successfully transformed the by-products of cuttlefish processing into value-added products. The process not only achieved the recycling and utilization of by-products but also enhanced industrial competitiveness and resource sustainability.https://www.mdpi.com/1996-1944/15/22/7969cuttlefishchitin/chitosanreaction surface method (RSM)<sup>1</sup>H NMRFTIR |
| spellingShingle | Sulfath Hakkim Hazeena Chih-Yao Hou Jing-Huei Zeng Bo-Heng Li Tzu-Chih Lin Cai-Sian Liu Chi-I Chang Shu-Ling Hsieh Ming-Kuei Shih Extraction Optimization and Structural Characteristics of Chitosan from Cuttlefish (<i>S. pharaonis</i> sp.) Bone Materials cuttlefish chitin/chitosan reaction surface method (RSM) <sup>1</sup>H NMR FTIR |
| title | Extraction Optimization and Structural Characteristics of Chitosan from Cuttlefish (<i>S. pharaonis</i> sp.) Bone |
| title_full | Extraction Optimization and Structural Characteristics of Chitosan from Cuttlefish (<i>S. pharaonis</i> sp.) Bone |
| title_fullStr | Extraction Optimization and Structural Characteristics of Chitosan from Cuttlefish (<i>S. pharaonis</i> sp.) Bone |
| title_full_unstemmed | Extraction Optimization and Structural Characteristics of Chitosan from Cuttlefish (<i>S. pharaonis</i> sp.) Bone |
| title_short | Extraction Optimization and Structural Characteristics of Chitosan from Cuttlefish (<i>S. pharaonis</i> sp.) Bone |
| title_sort | extraction optimization and structural characteristics of chitosan from cuttlefish i s pharaonis i sp bone |
| topic | cuttlefish chitin/chitosan reaction surface method (RSM) <sup>1</sup>H NMR FTIR |
| url | https://www.mdpi.com/1996-1944/15/22/7969 |
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