Transdifferentiation-Induced Neural Stem Cells Promote Recovery of Middle Cerebral Artery Stroke Rats.
Induced neural stem cells (iNSCs) can be directly transdifferentiated from somatic cells. One potential clinical application of the iNSCs is for nerve regeneration. However, it is unknown whether iNSCs function in disease models. We produced transdifferentiated iNSCs by conditional overexpressing Oc...
| Main Authors: | , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4564190?pdf=render |
| _version_ | 1818996616891203584 |
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| author | Hui Yao Mou Gao Jianhua Ma Maoying Zhang Shaowu Li Bingshan Wu Xiaohu Nie Jiao Jiao Hao Zhao Shanshan Wang Yuanyuan Yang Yesen Zhang Yilin Sun Max S Wicha Alfred E Chang Shaorong Gao Qiao Li Ruxiang Xu |
| author_facet | Hui Yao Mou Gao Jianhua Ma Maoying Zhang Shaowu Li Bingshan Wu Xiaohu Nie Jiao Jiao Hao Zhao Shanshan Wang Yuanyuan Yang Yesen Zhang Yilin Sun Max S Wicha Alfred E Chang Shaorong Gao Qiao Li Ruxiang Xu |
| author_sort | Hui Yao |
| collection | DOAJ |
| description | Induced neural stem cells (iNSCs) can be directly transdifferentiated from somatic cells. One potential clinical application of the iNSCs is for nerve regeneration. However, it is unknown whether iNSCs function in disease models. We produced transdifferentiated iNSCs by conditional overexpressing Oct4, Sox2, Klf4, c-Mycin mouse embryonic fibroblasts. They expanded readily in vitro and expressed NSC mRNA profile and protein markers. These iNSCs differentiated into mature astrocytes, neurons and oligodendrocytes in vitro. Importantly, they reduced lesion size, promoted the recovery of motor and sensory function as well as metabolism status in middle cerebral artery stroke rats. These iNSCs secreted nerve growth factors, which was associated with observed protection of neurons from apoptosis. Furthermore, iNSCs migrated to and passed through the lesion in the cerebral cortex, where Tuj1+ neurons were detected. These findings have revealed the function of transdifferentiated iNSCs in vivo, and thus provide experimental evidence to support the development of personalized regenerative therapy for CNS diseases by using genetically engineered autologous somatic cells. |
| first_indexed | 2024-12-20T21:32:35Z |
| format | Article |
| id | doaj.art-ba740262df264627be6746d0c0a74bfd |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T21:32:35Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-ba740262df264627be6746d0c0a74bfd2022-12-21T19:26:01ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01109e013721110.1371/journal.pone.0137211Transdifferentiation-Induced Neural Stem Cells Promote Recovery of Middle Cerebral Artery Stroke Rats.Hui YaoMou GaoJianhua MaMaoying ZhangShaowu LiBingshan WuXiaohu NieJiao JiaoHao ZhaoShanshan WangYuanyuan YangYesen ZhangYilin SunMax S WichaAlfred E ChangShaorong GaoQiao LiRuxiang XuInduced neural stem cells (iNSCs) can be directly transdifferentiated from somatic cells. One potential clinical application of the iNSCs is for nerve regeneration. However, it is unknown whether iNSCs function in disease models. We produced transdifferentiated iNSCs by conditional overexpressing Oct4, Sox2, Klf4, c-Mycin mouse embryonic fibroblasts. They expanded readily in vitro and expressed NSC mRNA profile and protein markers. These iNSCs differentiated into mature astrocytes, neurons and oligodendrocytes in vitro. Importantly, they reduced lesion size, promoted the recovery of motor and sensory function as well as metabolism status in middle cerebral artery stroke rats. These iNSCs secreted nerve growth factors, which was associated with observed protection of neurons from apoptosis. Furthermore, iNSCs migrated to and passed through the lesion in the cerebral cortex, where Tuj1+ neurons were detected. These findings have revealed the function of transdifferentiated iNSCs in vivo, and thus provide experimental evidence to support the development of personalized regenerative therapy for CNS diseases by using genetically engineered autologous somatic cells.http://europepmc.org/articles/PMC4564190?pdf=render |
| spellingShingle | Hui Yao Mou Gao Jianhua Ma Maoying Zhang Shaowu Li Bingshan Wu Xiaohu Nie Jiao Jiao Hao Zhao Shanshan Wang Yuanyuan Yang Yesen Zhang Yilin Sun Max S Wicha Alfred E Chang Shaorong Gao Qiao Li Ruxiang Xu Transdifferentiation-Induced Neural Stem Cells Promote Recovery of Middle Cerebral Artery Stroke Rats. PLoS ONE |
| title | Transdifferentiation-Induced Neural Stem Cells Promote Recovery of Middle Cerebral Artery Stroke Rats. |
| title_full | Transdifferentiation-Induced Neural Stem Cells Promote Recovery of Middle Cerebral Artery Stroke Rats. |
| title_fullStr | Transdifferentiation-Induced Neural Stem Cells Promote Recovery of Middle Cerebral Artery Stroke Rats. |
| title_full_unstemmed | Transdifferentiation-Induced Neural Stem Cells Promote Recovery of Middle Cerebral Artery Stroke Rats. |
| title_short | Transdifferentiation-Induced Neural Stem Cells Promote Recovery of Middle Cerebral Artery Stroke Rats. |
| title_sort | transdifferentiation induced neural stem cells promote recovery of middle cerebral artery stroke rats |
| url | http://europepmc.org/articles/PMC4564190?pdf=render |
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