Genetic architecture of berry aroma compounds in a QTL (quantitative trait loci) mapping population of interspecific hybrid grapes (Vitis labruscana × Vitis vinifera)
Abstract Background Although grapes accumulate diverse groups of volatile compounds, their genetic regulation in different cultivars remains unelucidated. Therefore, this study investigated the volatile composition in the berries of an interspecific hybrid population from a Vitis labruscana ‘Campbel...
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| Format: | Article |
| Language: | English |
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BMC
2022-09-01
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| Series: | BMC Plant Biology |
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| Online Access: | https://doi.org/10.1186/s12870-022-03842-z |
| _version_ | 1828175430976274432 |
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| author | Kazuya Koyama Atsushi Kono Yusuke Ban Sharon Marie Bahena-Garrido Tomoko Ohama Kazuhiro Iwashita Hisashi Fukuda Nami Goto-Yamamoto |
| author_facet | Kazuya Koyama Atsushi Kono Yusuke Ban Sharon Marie Bahena-Garrido Tomoko Ohama Kazuhiro Iwashita Hisashi Fukuda Nami Goto-Yamamoto |
| author_sort | Kazuya Koyama |
| collection | DOAJ |
| description | Abstract Background Although grapes accumulate diverse groups of volatile compounds, their genetic regulation in different cultivars remains unelucidated. Therefore, this study investigated the volatile composition in the berries of an interspecific hybrid population from a Vitis labruscana ‘Campbell Early’ (CE) × Vitis vinifera ‘Muscat of Alexandria’ (MA) cross to understand the relationship among volatile compounds and their genetic regulation. Then, a quantitative trait locus (QTL) analysis of its volatile compounds was conducted. Results While MA contained higher concentrations of monoterpenes and norisoprenoids, CE contained higher concentrations of C6 compounds, lactones and shikimic acid derivatives, including volatiles characteristic to American hybrids, i.e., methyl anthranilate, o-aminoacetophenone and mesifurane. Furthermore, a cluster analysis of volatile profiles in the hybrid population discovered ten coordinately modulated free and bound volatile clusters. QTL analysis identified a major QTL on linkage group (LG) 5 in the MA map for 14 monoterpene concentrations, consistent with a previously reported locus. Additionally, several QTLs detected in the CE map affected the concentrations of specific monoterpenes, such as linalool, citronellol and 1,8-cineol, modifying the monoterpene composition in the berries. As for the concentrations of five norisoprenoids, a major common QTL on LG2 was discovered first in this study. Several QTLs with minor effects were also discovered in various volatile groups, such as lactones, alcohols and shikimic acid derivatives. Conclusions An overview of the profiles of aroma compounds and their underlying QTLs in a population of interspecific hybrid grapes in which muscat flavor compounds and many other aroma compounds were mixed variously were elucidated. Coordinate modulation of the volatile clusters in the hybrid population suggested an independent mechanism for controlling the volatiles of each group. Accordingly, specific QTLs with significant effects were observed for terpenoids, norisoprenoids and some volatiles highly contained in CE berries. |
| first_indexed | 2024-04-12T04:28:12Z |
| format | Article |
| id | doaj.art-ba74709df4dd403ba1f4f5447c197d47 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-04-12T04:28:12Z |
| publishDate | 2022-09-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-ba74709df4dd403ba1f4f5447c197d472022-12-22T03:48:00ZengBMCBMC Plant Biology1471-22292022-09-0122112010.1186/s12870-022-03842-zGenetic architecture of berry aroma compounds in a QTL (quantitative trait loci) mapping population of interspecific hybrid grapes (Vitis labruscana × Vitis vinifera)Kazuya Koyama0Atsushi Kono1Yusuke Ban2Sharon Marie Bahena-Garrido3Tomoko Ohama4Kazuhiro Iwashita5Hisashi Fukuda6Nami Goto-Yamamoto7National Research Institute of BrewingInstitute of Fruit Tree and Tea Science, NAROWestern Region Agricultural Research Center (Kinki, Chugoku and Shikoku Regions), NARONational Research Institute of BrewingNational Research Institute of BrewingNational Research Institute of BrewingNational Research Institute of BrewingNational Research Institute of BrewingAbstract Background Although grapes accumulate diverse groups of volatile compounds, their genetic regulation in different cultivars remains unelucidated. Therefore, this study investigated the volatile composition in the berries of an interspecific hybrid population from a Vitis labruscana ‘Campbell Early’ (CE) × Vitis vinifera ‘Muscat of Alexandria’ (MA) cross to understand the relationship among volatile compounds and their genetic regulation. Then, a quantitative trait locus (QTL) analysis of its volatile compounds was conducted. Results While MA contained higher concentrations of monoterpenes and norisoprenoids, CE contained higher concentrations of C6 compounds, lactones and shikimic acid derivatives, including volatiles characteristic to American hybrids, i.e., methyl anthranilate, o-aminoacetophenone and mesifurane. Furthermore, a cluster analysis of volatile profiles in the hybrid population discovered ten coordinately modulated free and bound volatile clusters. QTL analysis identified a major QTL on linkage group (LG) 5 in the MA map for 14 monoterpene concentrations, consistent with a previously reported locus. Additionally, several QTLs detected in the CE map affected the concentrations of specific monoterpenes, such as linalool, citronellol and 1,8-cineol, modifying the monoterpene composition in the berries. As for the concentrations of five norisoprenoids, a major common QTL on LG2 was discovered first in this study. Several QTLs with minor effects were also discovered in various volatile groups, such as lactones, alcohols and shikimic acid derivatives. Conclusions An overview of the profiles of aroma compounds and their underlying QTLs in a population of interspecific hybrid grapes in which muscat flavor compounds and many other aroma compounds were mixed variously were elucidated. Coordinate modulation of the volatile clusters in the hybrid population suggested an independent mechanism for controlling the volatiles of each group. Accordingly, specific QTLs with significant effects were observed for terpenoids, norisoprenoids and some volatiles highly contained in CE berries.https://doi.org/10.1186/s12870-022-03842-zVolatile compoundsQuantitative trait locus (QTL) analysisVitis labruscanaVitis viniferaSecondary metabolismTerpenoids |
| spellingShingle | Kazuya Koyama Atsushi Kono Yusuke Ban Sharon Marie Bahena-Garrido Tomoko Ohama Kazuhiro Iwashita Hisashi Fukuda Nami Goto-Yamamoto Genetic architecture of berry aroma compounds in a QTL (quantitative trait loci) mapping population of interspecific hybrid grapes (Vitis labruscana × Vitis vinifera) BMC Plant Biology Volatile compounds Quantitative trait locus (QTL) analysis Vitis labruscana Vitis vinifera Secondary metabolism Terpenoids |
| title | Genetic architecture of berry aroma compounds in a QTL (quantitative trait loci) mapping population of interspecific hybrid grapes (Vitis labruscana × Vitis vinifera) |
| title_full | Genetic architecture of berry aroma compounds in a QTL (quantitative trait loci) mapping population of interspecific hybrid grapes (Vitis labruscana × Vitis vinifera) |
| title_fullStr | Genetic architecture of berry aroma compounds in a QTL (quantitative trait loci) mapping population of interspecific hybrid grapes (Vitis labruscana × Vitis vinifera) |
| title_full_unstemmed | Genetic architecture of berry aroma compounds in a QTL (quantitative trait loci) mapping population of interspecific hybrid grapes (Vitis labruscana × Vitis vinifera) |
| title_short | Genetic architecture of berry aroma compounds in a QTL (quantitative trait loci) mapping population of interspecific hybrid grapes (Vitis labruscana × Vitis vinifera) |
| title_sort | genetic architecture of berry aroma compounds in a qtl quantitative trait loci mapping population of interspecific hybrid grapes vitis labruscana vitis vinifera |
| topic | Volatile compounds Quantitative trait locus (QTL) analysis Vitis labruscana Vitis vinifera Secondary metabolism Terpenoids |
| url | https://doi.org/10.1186/s12870-022-03842-z |
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