The Intake of a Cafeteria Diet in Nursing Rats Alters the Breast Milk Concentration of Proteins Important for the Development of Offspring
We aimed to analyse the effects of maternal intake of an unbalanced diet during lactation in the composition and the levels of proteins present in milk. Milk samples from control nursing dams (C-dams) or from nursing dams fed a cafeteria diet during lactation (CAF-dams) were obtained. We conducted a...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-08-01
|
| Series: | Nutrients |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2072-6643/12/8/2470 |
| _version_ | 1797557742807810048 |
|---|---|
| author | Catalina Amadora Pomar Juana Sánchez Andreu Palou |
| author_facet | Catalina Amadora Pomar Juana Sánchez Andreu Palou |
| author_sort | Catalina Amadora Pomar |
| collection | DOAJ |
| description | We aimed to analyse the effects of maternal intake of an unbalanced diet during lactation in the composition and the levels of proteins present in milk. Milk samples from control nursing dams (C-dams) or from nursing dams fed a cafeteria diet during lactation (CAF-dams) were obtained. We conducted a proteomic approach to identify significantly altered proteins in breast milk of C- and CAF-dams, and evaluated the levels of leptin, adiponectin and irisin for their implication in energy homeostasis. One-dimensional SDS-polyacrylamide gel electrophoresis (SDS-PAGE), followed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), revealed that the bands that presented a lower intensity in CAF-dams than control contain some caseins (α-S1-casein, α-S2-casein like B, and β-casein), α-lactalbumin and haptoglobin. Leptin and adiponectin levels were greater in the breast milk of CAF-dams than in controls, while levels of irisin were lower. In summary, the relative concentration of bioactive peptides was influenced by maternal diet consumption during lactation; these changes at early stages of life could influence the phenotypic traits of the offspring. |
| first_indexed | 2024-03-10T17:20:44Z |
| format | Article |
| id | doaj.art-ba761c6c77c5457697df8ab4c3bbb576 |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-10T17:20:44Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-ba761c6c77c5457697df8ab4c3bbb5762023-11-20T10:21:59ZengMDPI AGNutrients2072-66432020-08-01128247010.3390/nu12082470The Intake of a Cafeteria Diet in Nursing Rats Alters the Breast Milk Concentration of Proteins Important for the Development of OffspringCatalina Amadora Pomar0Juana Sánchez1Andreu Palou2Laboratory of Molecular Biology, Nutrition and Biotechnology (Nutrigenomics and Obesity), University of the Balearic Islands, 07122 Palma, SpainLaboratory of Molecular Biology, Nutrition and Biotechnology (Nutrigenomics and Obesity), University of the Balearic Islands, 07122 Palma, SpainLaboratory of Molecular Biology, Nutrition and Biotechnology (Nutrigenomics and Obesity), University of the Balearic Islands, 07122 Palma, SpainWe aimed to analyse the effects of maternal intake of an unbalanced diet during lactation in the composition and the levels of proteins present in milk. Milk samples from control nursing dams (C-dams) or from nursing dams fed a cafeteria diet during lactation (CAF-dams) were obtained. We conducted a proteomic approach to identify significantly altered proteins in breast milk of C- and CAF-dams, and evaluated the levels of leptin, adiponectin and irisin for their implication in energy homeostasis. One-dimensional SDS-polyacrylamide gel electrophoresis (SDS-PAGE), followed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), revealed that the bands that presented a lower intensity in CAF-dams than control contain some caseins (α-S1-casein, α-S2-casein like B, and β-casein), α-lactalbumin and haptoglobin. Leptin and adiponectin levels were greater in the breast milk of CAF-dams than in controls, while levels of irisin were lower. In summary, the relative concentration of bioactive peptides was influenced by maternal diet consumption during lactation; these changes at early stages of life could influence the phenotypic traits of the offspring.https://www.mdpi.com/2072-6643/12/8/2470milk compositionbioactive proteinslactation |
| spellingShingle | Catalina Amadora Pomar Juana Sánchez Andreu Palou The Intake of a Cafeteria Diet in Nursing Rats Alters the Breast Milk Concentration of Proteins Important for the Development of Offspring Nutrients milk composition bioactive proteins lactation |
| title | The Intake of a Cafeteria Diet in Nursing Rats Alters the Breast Milk Concentration of Proteins Important for the Development of Offspring |
| title_full | The Intake of a Cafeteria Diet in Nursing Rats Alters the Breast Milk Concentration of Proteins Important for the Development of Offspring |
| title_fullStr | The Intake of a Cafeteria Diet in Nursing Rats Alters the Breast Milk Concentration of Proteins Important for the Development of Offspring |
| title_full_unstemmed | The Intake of a Cafeteria Diet in Nursing Rats Alters the Breast Milk Concentration of Proteins Important for the Development of Offspring |
| title_short | The Intake of a Cafeteria Diet in Nursing Rats Alters the Breast Milk Concentration of Proteins Important for the Development of Offspring |
| title_sort | intake of a cafeteria diet in nursing rats alters the breast milk concentration of proteins important for the development of offspring |
| topic | milk composition bioactive proteins lactation |
| url | https://www.mdpi.com/2072-6643/12/8/2470 |
| work_keys_str_mv | AT catalinaamadorapomar theintakeofacafeteriadietinnursingratsaltersthebreastmilkconcentrationofproteinsimportantforthedevelopmentofoffspring AT juanasanchez theintakeofacafeteriadietinnursingratsaltersthebreastmilkconcentrationofproteinsimportantforthedevelopmentofoffspring AT andreupalou theintakeofacafeteriadietinnursingratsaltersthebreastmilkconcentrationofproteinsimportantforthedevelopmentofoffspring AT catalinaamadorapomar intakeofacafeteriadietinnursingratsaltersthebreastmilkconcentrationofproteinsimportantforthedevelopmentofoffspring AT juanasanchez intakeofacafeteriadietinnursingratsaltersthebreastmilkconcentrationofproteinsimportantforthedevelopmentofoffspring AT andreupalou intakeofacafeteriadietinnursingratsaltersthebreastmilkconcentrationofproteinsimportantforthedevelopmentofoffspring |