Inhibitory effects on the HMG-CoA Reductase in the chemical constituents of the Cassia mimosoides Linn
Cassia mimosoides Linn has been used from ancient times and used for treating hepatitis for its supposedly medically beneficial properties. In this study, different constituents of the Cassia mimosoides Linn (β-Sitosterol, Oleanolic Acid, Emodin, Carotene, Resorcinol, Luteolin, and α-L-Rhamnose) wer...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Sciendo
2016-12-01
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| Series: | Romanian Journal of Laboratory Medicine |
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| Online Access: | https://doi.org/10.1515/rrlm-2016-0041 |
| _version_ | 1818583973325963264 |
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| author | Shen Chuangpeng Huang Liping Xiang Hua Deng Minzhen Gao Huahong Zhu Zhangzhi Liu Min Luo Guangbo |
| author_facet | Shen Chuangpeng Huang Liping Xiang Hua Deng Minzhen Gao Huahong Zhu Zhangzhi Liu Min Luo Guangbo |
| author_sort | Shen Chuangpeng |
| collection | DOAJ |
| description | Cassia mimosoides Linn has been used from ancient times and used for treating hepatitis for its supposedly medically beneficial properties. In this study, different constituents of the Cassia mimosoides Linn (β-Sitosterol, Oleanolic Acid, Emodin, Carotene, Resorcinol, Luteolin, and α-L-Rhamnose) were evaluated for potential anti-HMG-CoA reductase effect. The inhibitory effects of HMG-CoA reductase of Cassia mimosoides Linn extracts and Pravastatin inhibitor at different concentrations (at doses of 1, 5, 25 or 125 μg/mL, respectively) in reaction system (70 mmol/L phosphate buffer, 200mmol/L NADPH, 5 μg HMG-CoA reductase, 2 mmol/L EDTA, 2 mmol/L cysteamine, 0.06% BSA) into 37°C preheat HMG-CoA for initiating this reaction, and then determined the change of HMG-CoA reductase activity (ΔAΔt) at 340 nm, the inhibition ratio of HMG-CoA reductase activity and its dynamic change of inhibitory effect within 15 min and the descent rate of NADPH. Emodin, Luteolin, β-Sitosterol, Oleanolic Acid, α-L-Rhamnose and Carotene showed good inhibition of HMG-CoA reductase activity. Among them, only the Emodin (1 and 5 μg/mL) groups showed a significant decrease of HMG-CoA reductase activity compared to the Pravastatin (1 and 5 μg/mL) groups respectively. In addition, the HMG-CoA reductase activity in the Emodin and Luteolin (25 and 125 μg/mL) groups was clearly lower than the Pravastatin (25 and 125 μg/mL) groups respectively. And the Emodin and Luteolin (1, 5, 25 or 125 μg/mL) groups exhibited a stable effect on inhibiting the HMG-CoA reductase within 15 min. These findings further support the exploration of Cassia mimosoides Linn as a potential agent for the treatment of hepatitis in future studies. |
| first_indexed | 2024-12-16T08:13:47Z |
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| id | doaj.art-ba77fd44e0b0480c9617978fc9843e94 |
| institution | Directory Open Access Journal |
| issn | 2284-5623 |
| language | English |
| last_indexed | 2024-12-16T08:13:47Z |
| publishDate | 2016-12-01 |
| publisher | Sciendo |
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| series | Romanian Journal of Laboratory Medicine |
| spelling | doaj.art-ba77fd44e0b0480c9617978fc9843e942022-12-21T22:38:19ZengSciendoRomanian Journal of Laboratory Medicine2284-56232016-12-0124441342210.1515/rrlm-2016-0041Inhibitory effects on the HMG-CoA Reductase in the chemical constituents of the Cassia mimosoides LinnShen Chuangpeng0Huang Liping1Xiang Hua2Deng Minzhen3Gao Huahong4Zhu Zhangzhi5Liu Min6Luo Guangbo7Department of Endocrinology, the First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou Guangdong, 510405, P.R. ChinaLingnan Normal University, Zhanjiang Guangdong, 524048, P.R. ChinaYangChun People’s Hosptial, YangChun Guangdong, 529600, P.R. ChinaGuangdong Provincial Hospital of Chinese Medicine, Guangdong Provincial Academy of Chinese Medical Sciences, and The Second Clinical College, Guangzhou University of Chinese Medicine, Guangzhou 510120, P.R. ChinaSun Yat-sen University, Guangzhou Guangdong, 510275, P.R. ChinaDepartment of Endocrinology, the First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou Guangdong, 510405, P.R. ChinaDepartment of Endocrinology, the First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou Guangdong, 510405, P.R. ChinaDepartment of Endocrinology, the First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou Guangdong, 510405, P.R. ChinaCassia mimosoides Linn has been used from ancient times and used for treating hepatitis for its supposedly medically beneficial properties. In this study, different constituents of the Cassia mimosoides Linn (β-Sitosterol, Oleanolic Acid, Emodin, Carotene, Resorcinol, Luteolin, and α-L-Rhamnose) were evaluated for potential anti-HMG-CoA reductase effect. The inhibitory effects of HMG-CoA reductase of Cassia mimosoides Linn extracts and Pravastatin inhibitor at different concentrations (at doses of 1, 5, 25 or 125 μg/mL, respectively) in reaction system (70 mmol/L phosphate buffer, 200mmol/L NADPH, 5 μg HMG-CoA reductase, 2 mmol/L EDTA, 2 mmol/L cysteamine, 0.06% BSA) into 37°C preheat HMG-CoA for initiating this reaction, and then determined the change of HMG-CoA reductase activity (ΔAΔt) at 340 nm, the inhibition ratio of HMG-CoA reductase activity and its dynamic change of inhibitory effect within 15 min and the descent rate of NADPH. Emodin, Luteolin, β-Sitosterol, Oleanolic Acid, α-L-Rhamnose and Carotene showed good inhibition of HMG-CoA reductase activity. Among them, only the Emodin (1 and 5 μg/mL) groups showed a significant decrease of HMG-CoA reductase activity compared to the Pravastatin (1 and 5 μg/mL) groups respectively. In addition, the HMG-CoA reductase activity in the Emodin and Luteolin (25 and 125 μg/mL) groups was clearly lower than the Pravastatin (25 and 125 μg/mL) groups respectively. And the Emodin and Luteolin (1, 5, 25 or 125 μg/mL) groups exhibited a stable effect on inhibiting the HMG-CoA reductase within 15 min. These findings further support the exploration of Cassia mimosoides Linn as a potential agent for the treatment of hepatitis in future studies.https://doi.org/10.1515/rrlm-2016-0041cassia mimosoides linn3-hydroxy-3-methylglutaryl-coa reductaseemodinluteolinchinese herbal medicines |
| spellingShingle | Shen Chuangpeng Huang Liping Xiang Hua Deng Minzhen Gao Huahong Zhu Zhangzhi Liu Min Luo Guangbo Inhibitory effects on the HMG-CoA Reductase in the chemical constituents of the Cassia mimosoides Linn Romanian Journal of Laboratory Medicine cassia mimosoides linn 3-hydroxy-3-methylglutaryl-coa reductase emodin luteolin chinese herbal medicines |
| title | Inhibitory effects on the HMG-CoA Reductase in the chemical constituents of the Cassia mimosoides Linn |
| title_full | Inhibitory effects on the HMG-CoA Reductase in the chemical constituents of the Cassia mimosoides Linn |
| title_fullStr | Inhibitory effects on the HMG-CoA Reductase in the chemical constituents of the Cassia mimosoides Linn |
| title_full_unstemmed | Inhibitory effects on the HMG-CoA Reductase in the chemical constituents of the Cassia mimosoides Linn |
| title_short | Inhibitory effects on the HMG-CoA Reductase in the chemical constituents of the Cassia mimosoides Linn |
| title_sort | inhibitory effects on the hmg coa reductase in the chemical constituents of the cassia mimosoides linn |
| topic | cassia mimosoides linn 3-hydroxy-3-methylglutaryl-coa reductase emodin luteolin chinese herbal medicines |
| url | https://doi.org/10.1515/rrlm-2016-0041 |
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