Endothelial cell self-fusion during vascular pruning.
During embryonic development, vascular networks remodel to meet the increasing demand of growing tissues for oxygen and nutrients. This is achieved by the pruning of redundant blood vessel segments, which then allows more efficient blood flow patterns. Because of the lack of an in vivo system suitab...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2015-04-01
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| Series: | PLoS Biology |
| Online Access: | https://doi.org/10.1371/journal.pbio.1002126 |
| _version_ | 1819289574238584832 |
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| author | Anna Lenard Stephan Daetwyler Charles Betz Elin Ellertsdottir Heinz-Georg Belting Jan Huisken Markus Affolter |
| author_facet | Anna Lenard Stephan Daetwyler Charles Betz Elin Ellertsdottir Heinz-Georg Belting Jan Huisken Markus Affolter |
| author_sort | Anna Lenard |
| collection | DOAJ |
| description | During embryonic development, vascular networks remodel to meet the increasing demand of growing tissues for oxygen and nutrients. This is achieved by the pruning of redundant blood vessel segments, which then allows more efficient blood flow patterns. Because of the lack of an in vivo system suitable for high-resolution live imaging, the dynamics of the pruning process have not been described in detail. Here, we present the subintestinal vein (SIV) plexus of the zebrafish embryo as a novel model to study pruning at the cellular level. We show that blood vessel regression is a coordinated process of cell rearrangements involving lumen collapse and cell-cell contact resolution. Interestingly, the cellular rearrangements during pruning resemble endothelial cell behavior during vessel fusion in a reversed order. In pruning segments, endothelial cells first migrate toward opposing sides where they join the parental vascular branches, thus remodeling the multicellular segment into a unicellular connection. Often, the lumen is maintained throughout this process, and transient unicellular tubes form through cell self-fusion. In a second step, the unicellular connection is resolved unilaterally, and the pruning cell rejoins the opposing branch. Thus, we show for the first time that various cellular activities are coordinated to achieve blood vessel pruning and define two different morphogenetic pathways, which are selected by the flow environment. |
| first_indexed | 2024-12-24T03:09:01Z |
| format | Article |
| id | doaj.art-ba7b4ea1497f4f8183dbaebf9e9bdd97 |
| institution | Directory Open Access Journal |
| issn | 1544-9173 1545-7885 |
| language | English |
| last_indexed | 2024-12-24T03:09:01Z |
| publishDate | 2015-04-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Biology |
| spelling | doaj.art-ba7b4ea1497f4f8183dbaebf9e9bdd972022-12-21T17:17:53ZengPublic Library of Science (PLoS)PLoS Biology1544-91731545-78852015-04-01134e100212610.1371/journal.pbio.1002126Endothelial cell self-fusion during vascular pruning.Anna LenardStephan DaetwylerCharles BetzElin EllertsdottirHeinz-Georg BeltingJan HuiskenMarkus AffolterDuring embryonic development, vascular networks remodel to meet the increasing demand of growing tissues for oxygen and nutrients. This is achieved by the pruning of redundant blood vessel segments, which then allows more efficient blood flow patterns. Because of the lack of an in vivo system suitable for high-resolution live imaging, the dynamics of the pruning process have not been described in detail. Here, we present the subintestinal vein (SIV) plexus of the zebrafish embryo as a novel model to study pruning at the cellular level. We show that blood vessel regression is a coordinated process of cell rearrangements involving lumen collapse and cell-cell contact resolution. Interestingly, the cellular rearrangements during pruning resemble endothelial cell behavior during vessel fusion in a reversed order. In pruning segments, endothelial cells first migrate toward opposing sides where they join the parental vascular branches, thus remodeling the multicellular segment into a unicellular connection. Often, the lumen is maintained throughout this process, and transient unicellular tubes form through cell self-fusion. In a second step, the unicellular connection is resolved unilaterally, and the pruning cell rejoins the opposing branch. Thus, we show for the first time that various cellular activities are coordinated to achieve blood vessel pruning and define two different morphogenetic pathways, which are selected by the flow environment.https://doi.org/10.1371/journal.pbio.1002126 |
| spellingShingle | Anna Lenard Stephan Daetwyler Charles Betz Elin Ellertsdottir Heinz-Georg Belting Jan Huisken Markus Affolter Endothelial cell self-fusion during vascular pruning. PLoS Biology |
| title | Endothelial cell self-fusion during vascular pruning. |
| title_full | Endothelial cell self-fusion during vascular pruning. |
| title_fullStr | Endothelial cell self-fusion during vascular pruning. |
| title_full_unstemmed | Endothelial cell self-fusion during vascular pruning. |
| title_short | Endothelial cell self-fusion during vascular pruning. |
| title_sort | endothelial cell self fusion during vascular pruning |
| url | https://doi.org/10.1371/journal.pbio.1002126 |
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