A rapid test for detection of tribenuron-methyl resistance in sunflower
A rapid laboratory test for detection of tribenuron-methyl resistant sunflower genotypes was developed. Four homozygous and four heterozygous tribenuron-methyl resistant hybrids, as well as a susceptible one were grown on MS medium supplemented with different concentrations of herbicide (2.0 µM, 2.5...
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| Format: | Article |
| Language: | English |
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Institute of field and vegetable crops, Novi Sad
2016-01-01
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| Series: | Ratarstvo i Povrtarstvo |
| Subjects: | |
| Online Access: | http://scindeks-clanci.ceon.rs/data/pdf/1821-3944/2016/1821-39441601001D.pdf |
| _version_ | 1818310276829675520 |
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| author | Dimitrijević Aleksandra Imerovski Ivana Miladinović Dragana Jocić Siniša Cvejić Sandra Malidža Goran Kovačević Branislav Šurlan-Momirović Gordana |
| author_facet | Dimitrijević Aleksandra Imerovski Ivana Miladinović Dragana Jocić Siniša Cvejić Sandra Malidža Goran Kovačević Branislav Šurlan-Momirović Gordana |
| author_sort | Dimitrijević Aleksandra |
| collection | DOAJ |
| description | A rapid laboratory test for detection of tribenuron-methyl resistant sunflower genotypes was developed. Four homozygous and four heterozygous tribenuron-methyl resistant hybrids, as well as a susceptible one were grown on MS medium supplemented with different concentrations of herbicide (2.0 µM, 2.5 µM, 3.0 µM, 3.5 µM and 4.0 µM) and with pH either 7 or 8. The effect of medium pH and herbicide concentration on above-ground part and root mass of sunflower seedlings was observed. The test enabled visual discrimination between resistant and susceptible genotypes in 5 days, and discrimination between homozygous and heterozygous resistant genotypes in 12 days. All tested herbicide concentrations and morphological parameters were found to be suitable for discrimination of tribenuron-methyl susceptible genotypes. The best pH and herbicide concentration combination and morphological parameter for discrimination between homozygous and heterozygous resistant sunflower genotypes were 3.0 µM at pH 7 and root mass, respectively. |
| first_indexed | 2024-12-13T07:43:30Z |
| format | Article |
| id | doaj.art-ba7b735c37144b02801a52eee8862f8b |
| institution | Directory Open Access Journal |
| issn | 1821-3944 2217-8392 |
| language | English |
| last_indexed | 2024-12-13T07:43:30Z |
| publishDate | 2016-01-01 |
| publisher | Institute of field and vegetable crops, Novi Sad |
| record_format | Article |
| series | Ratarstvo i Povrtarstvo |
| spelling | doaj.art-ba7b735c37144b02801a52eee8862f8b2022-12-21T23:54:55ZengInstitute of field and vegetable crops, Novi SadRatarstvo i Povrtarstvo1821-39442217-83922016-01-015311810.5937/ratpov53-84121821-39441601001DA rapid test for detection of tribenuron-methyl resistance in sunflowerDimitrijević Aleksandra0Imerovski Ivana1Miladinović Dragana2Jocić Siniša3Cvejić Sandra4Malidža Goran5Kovačević Branislav6Šurlan-Momirović Gordana7Crop and Vegetable Scientific Institute, Novi Sad, SerbiaCrop and Vegetable Scientific Institute, Novi Sad, SerbiaCrop and Vegetable Scientific Institute, Novi Sad, SerbiaCrop and Vegetable Scientific Institute, Novi Sad, SerbiaCrop and Vegetable Scientific Institute, Novi Sad, SerbiaCrop and Vegetable Scientific Institute, Novi Sad, SerbiaInstitute of Lowland Forestry and Environment, Novi Sad, Novi Sad, SerbiaUniversity of Belgrade, Faculty of Agriculture, Belgrade, SerbiaA rapid laboratory test for detection of tribenuron-methyl resistant sunflower genotypes was developed. Four homozygous and four heterozygous tribenuron-methyl resistant hybrids, as well as a susceptible one were grown on MS medium supplemented with different concentrations of herbicide (2.0 µM, 2.5 µM, 3.0 µM, 3.5 µM and 4.0 µM) and with pH either 7 or 8. The effect of medium pH and herbicide concentration on above-ground part and root mass of sunflower seedlings was observed. The test enabled visual discrimination between resistant and susceptible genotypes in 5 days, and discrimination between homozygous and heterozygous resistant genotypes in 12 days. All tested herbicide concentrations and morphological parameters were found to be suitable for discrimination of tribenuron-methyl susceptible genotypes. The best pH and herbicide concentration combination and morphological parameter for discrimination between homozygous and heterozygous resistant sunflower genotypes were 3.0 µM at pH 7 and root mass, respectively.http://scindeks-clanci.ceon.rs/data/pdf/1821-3944/2016/1821-39441601001D.pdfbioassayHelianthus annuus L.herbicidesselectionsulfonylureastolerance |
| spellingShingle | Dimitrijević Aleksandra Imerovski Ivana Miladinović Dragana Jocić Siniša Cvejić Sandra Malidža Goran Kovačević Branislav Šurlan-Momirović Gordana A rapid test for detection of tribenuron-methyl resistance in sunflower Ratarstvo i Povrtarstvo bioassay Helianthus annuus L. herbicides selection sulfonylureas tolerance |
| title | A rapid test for detection of tribenuron-methyl resistance in sunflower |
| title_full | A rapid test for detection of tribenuron-methyl resistance in sunflower |
| title_fullStr | A rapid test for detection of tribenuron-methyl resistance in sunflower |
| title_full_unstemmed | A rapid test for detection of tribenuron-methyl resistance in sunflower |
| title_short | A rapid test for detection of tribenuron-methyl resistance in sunflower |
| title_sort | rapid test for detection of tribenuron methyl resistance in sunflower |
| topic | bioassay Helianthus annuus L. herbicides selection sulfonylureas tolerance |
| url | http://scindeks-clanci.ceon.rs/data/pdf/1821-3944/2016/1821-39441601001D.pdf |
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