Antioxidative Stress and Anti-aging Effects of Lithocarpus litseifolius folium Based on Caenorhabditis elegans Model
In this study, the main components of the extract of (Lithocarpus litseifolius folium extract, LLFE) were analyzed,and the anti-oxidative stress and anti-aging effects of LLFE on C.elegans were evaluated using Caenorhabditis elegans (C.elegans) as a model to explore the possible mechanisms of action...
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| Language: | zho |
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The editorial department of Science and Technology of Food Industry
2023-06-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022080282 |
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| author | Huikang ZHANG Jiabo MA Qi SI Zhenzhen WANG Ruyi SHA Jing DAI Jianwei MAO |
| author_facet | Huikang ZHANG Jiabo MA Qi SI Zhenzhen WANG Ruyi SHA Jing DAI Jianwei MAO |
| author_sort | Huikang ZHANG |
| collection | DOAJ |
| description | In this study, the main components of the extract of (Lithocarpus litseifolius folium extract, LLFE) were analyzed,and the anti-oxidative stress and anti-aging effects of LLFE on C.elegans were evaluated using Caenorhabditis elegans (C.elegans) as a model to explore the possible mechanisms of action.The results showed that LLFE contained 52.84±1.45 mg/g of total phenols, 380.74±15.12 mg/g of total flavonoids, 470.36±5.84 mg/g of total sugars and about 6.00% of dihydrochalcone compounds, with phloridzin 52.76±0.71 mg/g, phloretin 1.64±0.06 mg/g and trilobatin 4.88±0.01 mg/g. LLFE (0.25, 1.25 mg/mL) also significantly extended the mean lifespan of C.elegans under oxidative and heat stress conditions (P<0.05) and enhanced their own resistance to stress. After using H2O2-induced oxidative damage in C.elegans as a model, it was found that LLFE (0.05, 0.25 and 1.25 mg/mL) significantly increased the mean lifespan of C.elegans compared with the model group (P<0.05). When the LLFE concentration was 1.25 mg/mL, the total superoxide dismutase activity in C.elegans increased by 39.85% (P<0.05), catalase activity by 39.21%(P<0.05) and glutathione peroxidase activity by 66.95% (P<0.001). The accumulation of malondialdehyde and reactive oxygen species was also reduced by 66.05% (P<0.001) and 49.69% (P<0.001), respectively. This indicates that LLFE has good antioxidant effect, and then delay the process of aging. |
| first_indexed | 2024-03-12T13:34:41Z |
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| id | doaj.art-ba7f255ff5ba46238c0429c47a327d5b |
| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-03-12T13:34:41Z |
| publishDate | 2023-06-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
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| series | Shipin gongye ke-ji |
| spelling | doaj.art-ba7f255ff5ba46238c0429c47a327d5b2023-08-24T06:00:59ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062023-06-01441236337010.13386/j.issn1002-0306.20220802822022080282-12Antioxidative Stress and Anti-aging Effects of Lithocarpus litseifolius folium Based on Caenorhabditis elegans ModelHuikang ZHANG0Jiabo MA1Qi SI2Zhenzhen WANG3Ruyi SHA4Jing DAI5Jianwei MAO6Zhejiang Key Laboratory of Chemical and Bioprocessing Technology for Agricultural Products, Zhejiang Collaborative Innovation Center for Biochemical Manufacturing of Agricultural Bioresources, College of Biology and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, ChinaZhejiang Key Laboratory of Chemical and Bioprocessing Technology for Agricultural Products, Zhejiang Collaborative Innovation Center for Biochemical Manufacturing of Agricultural Bioresources, College of Biology and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, ChinaZhejiang Key Laboratory of Chemical and Bioprocessing Technology for Agricultural Products, Zhejiang Collaborative Innovation Center for Biochemical Manufacturing of Agricultural Bioresources, College of Biology and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, ChinaZhejiang Key Laboratory of Chemical and Bioprocessing Technology for Agricultural Products, Zhejiang Collaborative Innovation Center for Biochemical Manufacturing of Agricultural Bioresources, College of Biology and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, ChinaZhejiang Key Laboratory of Chemical and Bioprocessing Technology for Agricultural Products, Zhejiang Collaborative Innovation Center for Biochemical Manufacturing of Agricultural Bioresources, College of Biology and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, ChinaZhejiang Key Laboratory of Chemical and Bioprocessing Technology for Agricultural Products, Zhejiang Collaborative Innovation Center for Biochemical Manufacturing of Agricultural Bioresources, College of Biology and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, ChinaZhejiang Key Laboratory of Chemical and Bioprocessing Technology for Agricultural Products, Zhejiang Collaborative Innovation Center for Biochemical Manufacturing of Agricultural Bioresources, College of Biology and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, ChinaIn this study, the main components of the extract of (Lithocarpus litseifolius folium extract, LLFE) were analyzed,and the anti-oxidative stress and anti-aging effects of LLFE on C.elegans were evaluated using Caenorhabditis elegans (C.elegans) as a model to explore the possible mechanisms of action.The results showed that LLFE contained 52.84±1.45 mg/g of total phenols, 380.74±15.12 mg/g of total flavonoids, 470.36±5.84 mg/g of total sugars and about 6.00% of dihydrochalcone compounds, with phloridzin 52.76±0.71 mg/g, phloretin 1.64±0.06 mg/g and trilobatin 4.88±0.01 mg/g. LLFE (0.25, 1.25 mg/mL) also significantly extended the mean lifespan of C.elegans under oxidative and heat stress conditions (P<0.05) and enhanced their own resistance to stress. After using H2O2-induced oxidative damage in C.elegans as a model, it was found that LLFE (0.05, 0.25 and 1.25 mg/mL) significantly increased the mean lifespan of C.elegans compared with the model group (P<0.05). When the LLFE concentration was 1.25 mg/mL, the total superoxide dismutase activity in C.elegans increased by 39.85% (P<0.05), catalase activity by 39.21%(P<0.05) and glutathione peroxidase activity by 66.95% (P<0.001). The accumulation of malondialdehyde and reactive oxygen species was also reduced by 66.05% (P<0.001) and 49.69% (P<0.001), respectively. This indicates that LLFE has good antioxidant effect, and then delay the process of aging.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022080282dihydrochalconecaenorhabditis eleganslifespanoxidative stresslithocarpus polystachy folium |
| spellingShingle | Huikang ZHANG Jiabo MA Qi SI Zhenzhen WANG Ruyi SHA Jing DAI Jianwei MAO Antioxidative Stress and Anti-aging Effects of Lithocarpus litseifolius folium Based on Caenorhabditis elegans Model Shipin gongye ke-ji dihydrochalcone caenorhabditis elegans lifespan oxidative stress lithocarpus polystachy folium |
| title | Antioxidative Stress and Anti-aging Effects of Lithocarpus litseifolius folium Based on Caenorhabditis elegans Model |
| title_full | Antioxidative Stress and Anti-aging Effects of Lithocarpus litseifolius folium Based on Caenorhabditis elegans Model |
| title_fullStr | Antioxidative Stress and Anti-aging Effects of Lithocarpus litseifolius folium Based on Caenorhabditis elegans Model |
| title_full_unstemmed | Antioxidative Stress and Anti-aging Effects of Lithocarpus litseifolius folium Based on Caenorhabditis elegans Model |
| title_short | Antioxidative Stress and Anti-aging Effects of Lithocarpus litseifolius folium Based on Caenorhabditis elegans Model |
| title_sort | antioxidative stress and anti aging effects of lithocarpus litseifolius folium based on caenorhabditis elegans model |
| topic | dihydrochalcone caenorhabditis elegans lifespan oxidative stress lithocarpus polystachy folium |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022080282 |
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