A high-throughput phenotyping method for sugarcane rind penetrometer resistance and breaking force characterization by near-infrared spectroscopy
Abstract Background Sugarcane (Saccharum spp.) is the core crop for sugar and bioethanol production over the world. A major problem in sugarcane production is stalk lodging due to weak mechanical strength. Rind penetrometer resistance (RPR) and breaking force are two kinds of regular parameters for...
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| Format: | Article |
| Language: | English |
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BMC
2023-09-01
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| Series: | Plant Methods |
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| Online Access: | https://doi.org/10.1186/s13007-023-01076-0 |
| _version_ | 1797559424487784448 |
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| author | Yinjuan Shen Muhammad Adnan Fumin Ma Liyuan Kong Maoyao Wang Fuhong Jiang Qian Hu Wei Yao Yongfang Zhou Muqing Zhang Jiangfeng Huang |
| author_facet | Yinjuan Shen Muhammad Adnan Fumin Ma Liyuan Kong Maoyao Wang Fuhong Jiang Qian Hu Wei Yao Yongfang Zhou Muqing Zhang Jiangfeng Huang |
| author_sort | Yinjuan Shen |
| collection | DOAJ |
| description | Abstract Background Sugarcane (Saccharum spp.) is the core crop for sugar and bioethanol production over the world. A major problem in sugarcane production is stalk lodging due to weak mechanical strength. Rind penetrometer resistance (RPR) and breaking force are two kinds of regular parameters for mechanical strength characterization. However, due to the lack of efficient methods for determining RPR and breaking force in sugarcane, genetic approaches for improving these traits are generally limited. This study was designed to use near-infrared spectroscopy (NIRS) calibration assay to accurately assess mechanical strength on a high-throughput basis for the first time. Results Based on well-established laboratory measurements of sugarcane stalk internodes collected in the years 2019 and 2020, considerable variations in RPR and breaking force were observed in the stalk internodes. Following a standard NIRS calibration process, two online models were obtained with a high coefficient of determination (R 2 ) and the ratio of prediction to deviation (RPD) values during calibration, internal cross-validation, and external validation. Remarkably, the equation for RPR exhibited R 2 and RPD values as high as 0.997 and 17.70, as well as showing relatively low root mean square error values at 0.44 N mm−2 during global modeling, demonstrating excellent predictive performance. Conclusions This study delivered a successful attempt for rapid and precise prediction of rind penetrometer resistance and breaking force in sugarcane stalk by NIRS assay. These established models can be used to improve phenotyping jobs for sugarcane germplasm on a large scale. |
| first_indexed | 2024-03-10T17:46:11Z |
| format | Article |
| id | doaj.art-ba83f54b28d24d2a98af3a1907466440 |
| institution | Directory Open Access Journal |
| issn | 1746-4811 |
| language | English |
| last_indexed | 2024-03-10T17:46:11Z |
| publishDate | 2023-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Plant Methods |
| spelling | doaj.art-ba83f54b28d24d2a98af3a19074664402023-11-20T09:32:44ZengBMCPlant Methods1746-48112023-09-0119111310.1186/s13007-023-01076-0A high-throughput phenotyping method for sugarcane rind penetrometer resistance and breaking force characterization by near-infrared spectroscopyYinjuan Shen0Muhammad Adnan1Fumin Ma2Liyuan Kong3Maoyao Wang4Fuhong Jiang5Qian Hu6Wei Yao7Yongfang Zhou8Muqing Zhang9Jiangfeng Huang10State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi Key Laboratory of Sugarcane Biology, Province and Ministry Co-Sponsored Collaborative Innovation Center of Canesugar Industry, Academy of Sugarcane and Sugar Industry, College of Agriculture, Guangxi UniversityState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi Key Laboratory of Sugarcane Biology, Province and Ministry Co-Sponsored Collaborative Innovation Center of Canesugar Industry, Academy of Sugarcane and Sugar Industry, College of Agriculture, Guangxi UniversityState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi Key Laboratory of Sugarcane Biology, Province and Ministry Co-Sponsored Collaborative Innovation Center of Canesugar Industry, Academy of Sugarcane and Sugar Industry, College of Agriculture, Guangxi UniversityState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi Key Laboratory of Sugarcane Biology, Province and Ministry Co-Sponsored Collaborative Innovation Center of Canesugar Industry, Academy of Sugarcane and Sugar Industry, College of Agriculture, Guangxi UniversityState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi Key Laboratory of Sugarcane Biology, Province and Ministry Co-Sponsored Collaborative Innovation Center of Canesugar Industry, Academy of Sugarcane and Sugar Industry, College of Agriculture, Guangxi UniversityState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi Key Laboratory of Sugarcane Biology, Province and Ministry Co-Sponsored Collaborative Innovation Center of Canesugar Industry, Academy of Sugarcane and Sugar Industry, College of Agriculture, Guangxi UniversityState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi Key Laboratory of Sugarcane Biology, Province and Ministry Co-Sponsored Collaborative Innovation Center of Canesugar Industry, Academy of Sugarcane and Sugar Industry, College of Agriculture, Guangxi UniversityState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi Key Laboratory of Sugarcane Biology, Province and Ministry Co-Sponsored Collaborative Innovation Center of Canesugar Industry, Academy of Sugarcane and Sugar Industry, College of Agriculture, Guangxi UniversityNanning Sugar Industry Co., LTDState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi Key Laboratory of Sugarcane Biology, Province and Ministry Co-Sponsored Collaborative Innovation Center of Canesugar Industry, Academy of Sugarcane and Sugar Industry, College of Agriculture, Guangxi UniversityState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi Key Laboratory of Sugarcane Biology, Province and Ministry Co-Sponsored Collaborative Innovation Center of Canesugar Industry, Academy of Sugarcane and Sugar Industry, College of Agriculture, Guangxi UniversityAbstract Background Sugarcane (Saccharum spp.) is the core crop for sugar and bioethanol production over the world. A major problem in sugarcane production is stalk lodging due to weak mechanical strength. Rind penetrometer resistance (RPR) and breaking force are two kinds of regular parameters for mechanical strength characterization. However, due to the lack of efficient methods for determining RPR and breaking force in sugarcane, genetic approaches for improving these traits are generally limited. This study was designed to use near-infrared spectroscopy (NIRS) calibration assay to accurately assess mechanical strength on a high-throughput basis for the first time. Results Based on well-established laboratory measurements of sugarcane stalk internodes collected in the years 2019 and 2020, considerable variations in RPR and breaking force were observed in the stalk internodes. Following a standard NIRS calibration process, two online models were obtained with a high coefficient of determination (R 2 ) and the ratio of prediction to deviation (RPD) values during calibration, internal cross-validation, and external validation. Remarkably, the equation for RPR exhibited R 2 and RPD values as high as 0.997 and 17.70, as well as showing relatively low root mean square error values at 0.44 N mm−2 during global modeling, demonstrating excellent predictive performance. Conclusions This study delivered a successful attempt for rapid and precise prediction of rind penetrometer resistance and breaking force in sugarcane stalk by NIRS assay. These established models can be used to improve phenotyping jobs for sugarcane germplasm on a large scale.https://doi.org/10.1186/s13007-023-01076-0SugarcaneMechanical strengthRind penetrometer resistanceBreaking forcePhenotyping |
| spellingShingle | Yinjuan Shen Muhammad Adnan Fumin Ma Liyuan Kong Maoyao Wang Fuhong Jiang Qian Hu Wei Yao Yongfang Zhou Muqing Zhang Jiangfeng Huang A high-throughput phenotyping method for sugarcane rind penetrometer resistance and breaking force characterization by near-infrared spectroscopy Plant Methods Sugarcane Mechanical strength Rind penetrometer resistance Breaking force Phenotyping |
| title | A high-throughput phenotyping method for sugarcane rind penetrometer resistance and breaking force characterization by near-infrared spectroscopy |
| title_full | A high-throughput phenotyping method for sugarcane rind penetrometer resistance and breaking force characterization by near-infrared spectroscopy |
| title_fullStr | A high-throughput phenotyping method for sugarcane rind penetrometer resistance and breaking force characterization by near-infrared spectroscopy |
| title_full_unstemmed | A high-throughput phenotyping method for sugarcane rind penetrometer resistance and breaking force characterization by near-infrared spectroscopy |
| title_short | A high-throughput phenotyping method for sugarcane rind penetrometer resistance and breaking force characterization by near-infrared spectroscopy |
| title_sort | high throughput phenotyping method for sugarcane rind penetrometer resistance and breaking force characterization by near infrared spectroscopy |
| topic | Sugarcane Mechanical strength Rind penetrometer resistance Breaking force Phenotyping |
| url | https://doi.org/10.1186/s13007-023-01076-0 |
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